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Literature summary for 3.2.1.132 extracted from

  • Dennhart, N.; Fukamizo, T.; Brzezinski, R.; Lacombe-Harvey, M.E.; Letzel, T.
    Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry (2008), J. Biotechnol., 134, 253-260.
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants subcloned into vector pFD666 and expressed in Streptomyces lividans TK-24 Streptomyces sp. N174

Protein Variants

Protein Variants Comment Organism
D201A time-course is almost identical to that obtained by the wild-type Streptomyces sp. N174
D40G reduces activity to 2% Streptomyces sp. N174

Organism

Organism UniProt Comment Textmining
Streptomyces sp. N174
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by a two-step procedure involving ion exchange chromatography followed by gel filtration, to purity Streptomyces sp. N174

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
-
 Streptomyces sp. N174 GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + D-glucosamine
-
 ?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
-
 Streptomyces sp. N174 GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN
-
 ?
GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN + H2O
-
 Streptomyces sp. N174 GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
-
 ?
hexa-N-acetylchitohexaose + H2O
-
 Streptomyces sp. N174 ?
-
 ?
additional information does not hydrolyze GlcNbeta(1-4)GlcN or GlcNbeta(1-4)GlcNbeta(1-4)GlcN Streptomyces sp. N174 ?
-
 ?

Synonyms

Synonyms Comment Organism
endochitosanase
-
 Streptomyces sp. N174