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Information on EC 3.2.1.24 - alpha-mannosidase
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EC Tree
3.2.1.24 alpha-mannosidaseIUBMB Comments
Also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose.
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Word Map
- 3.2.1.24
- oligosaccharide
- mannose
- glycosidases
-
n-linked
- swainsonine
- hydrolases
- mannans
- n-glycans
-
concanavalin
- alpha-fucosidase
- xylanase
- beta-n-acetylglucosaminidase
- beta-d-galactosidase
- beta-hexosaminidase
- cellulase
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endo-beta-n-acetylglucosaminidase
-
high-mannose
- beta-d-glucosidase
- galactomannans
- 1-deoxymannojirimycin
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asparagine-linked
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jack
- beta-mannosidase
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exoglycosidase
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mannosylated
- man9glcnac2
- n-acetyl-beta-glucosaminidase
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locust
- beta-d-glucuronidase
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complex-type
- glucomannans
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konjac
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mannose-type
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hemicellulases
- glc3man9glcnac2
- castanospermine
-
oligomannosides
- alpha-d-galactosidase
- alpha-d-glucosidase
-
mannose-rich
- beta-xylosidase
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lipid-linked
- hexosaminidase
- beta-n-acetylhexosaminidase
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mannose-containing
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hybrid-type
- i-cell
- mucolipidosis
-
oligomannose
- analysis
- cellulosome
- diagnostics
- medicine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
alpha-mannosidase, mannanase, alpha-d-mannosidase, lysosomal alpha-mannosidase, alpha-mannosidase ii, golgi alpha-mannosidase ii, alpha1,2-mannosidase, alpha-1,2-mannosidase, alpha-man, man2c1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,2-alpha-D-mannosidase
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1,2-alpha-mannosidase
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acid alpha-mannosidase
acidic alpha-mannosidase
Alpha mannosidase 6A8B
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alpha-1,2-mannosidase
alpha-D-mannopyranosidase
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alpha-D-mannosidase
Alpha-D-mannoside mannohydrolase
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alpha-mannosidase
alpha-mannosidase I
AMAN
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class II alpha-mannosidase
exo-alpha-mannosidase
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GH38 alpha-mannosidase
GH38 class II alpha-mannosidase
Golgi alpha-mannosidase II
Golgi mannosidase IA
Golgi mannosidase IB
Laman
Lysosomal acid alpha-mannosidase
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Man1p
ManA
MGG_00994.6
neutral alpha-mannosidase
p-nitrophenyl-alpha-mannosidase
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Ssa-man
SSO3006
additional information
alpha-D-mannosidase
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alpha-mannosidase
acidic exoglycosidase, belonging to glycosylhydrolase family 38
Laman
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additional information
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the enzyme belongs to the class 1 alpha1,2-mannosidases of glycosylhydrolase family 47
additional information
the enzyme belongs to the family GH38 of alpha-mannosidases
additional information
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the enzyme belongs to the glycoside hydrolase family 38
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O = 2 alpha-D-mannopyranose
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2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O = 2 alpha-D-mannopyranose
also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose
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2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O = 2 alpha-D-mannopyranose
active site structure and catalytic residues, e.g. Asn515 involved in substrate binding, reaction mechanism and processing steps, overview
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Man9GlcNAc2-[protein] + 4 H2O = Man5GlcNAc2-[protein] + 4 beta-D-mannopyranose
mechanism: ordered equilibrium
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Man9GlcNAc2-[protein] + 4 H2O = Man5GlcNAc2-[protein] + 4 beta-D-mannopyranose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-mannoside mannohydrolase
Also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose.
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CAS REGISTRY NUMBER
COMMENTARY
9025-42-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-naphthyl-alpha-D-mannopyranoside + H2O
1-naphthol + alpha-D-mannopyranose
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?
2 Man9GlcNAc + 3 H2O
Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose
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products from unbound fraction of cobalt-chelating Sepharose
?
2,4-dinitrophenyl alpha-D-mannoside + H2O
2,4-dinitrophenol + alpha-D-mannose
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-
-
-
?
2,4-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannopyranose
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-
-
-
?
2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,5-dinitrophenol + D-mannose
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-
-
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?
2-acetamido-4-O-alpha-D-mannopyranosyl-2-deoxy-D-glucose + H2O
alpha-D-mannopyranose + ?
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-
-
?
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride + H2O
?
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substrate for wild-type enzyme and very slow substrate of mutant enzyme D341N
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-
?
2-O-alpha-D-mannotriose + H2O
alpha-D-mannopyranose + ?
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highly specific for alpha-1,2-mannosidic linkages
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?
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
alpha-D-mannopyranose
the enzyme is only active with the alpha-1,3-linked disaccharide
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?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferol + alpha-D-mannopyranose
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannose
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pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3
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-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannose
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?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
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?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenyl + alpha-D-mannose
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?
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
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specific cleavage of alpha-1,4- or alpha-1,6-linkages
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?
5-fluoro-beta-L-gulosyl fluoride + H2O
?
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acts as a slow substrate for the D341 mutant enzyme, with deglycosylation as the rate-limiting step. Inactivation is only observed when assayed at low temperatures such that deglycosylation is slow relative to the assay time
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?
6 pyridylamino-Man9GlcNAc2 + H2O
pyridylamino-Man8GlcNAc2 + pyridylamino-Man7GlcNAc2 + pyridylamino-Man6GlcNAc2 + pyridylamino-Man5GlcNAc2 + pyridylamino-Man4GlcNAc2 + pyridylamino-Man3GlcNAc2 + 21 alpha-D-mannopyranose
6-bromo-2-naphthyl-alpha-D-mannopyranoside + H2O
6-bromo-2-naphthol + alpha-D-mannopyranose
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?
alpha-(1-2)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
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-
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?
alpha-(1-3)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
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highest activity and affinity
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?
alpha-(1-6)-mannobiose
alpha-D-mannopyranose + D-mannopyranose
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low activity
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?
alpha-conglutin + H2O
alpha-D-mannopyranose + ?
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lupin seed storage glycoprotein, not active to beta- or gamma-conglutin
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?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O
alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
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cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2
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?
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
alpha-D-mannose + ?
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
alpha-D-mannose + ?
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?
alpha-D-mannosidosis fibroblasts + H2O
Manbeta(1-4)GlcNAc + ?
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?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
?
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?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha(1-3)]-Man-beta-(1-4)-GlcNAc + alpha-mannose
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end product in presence of Co2+. Before treatment with Co(II) the enzyme is able to cleave a single Manalpha1-2 residue from the substrate to give Man-alpha(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]Man-beta-(1-4)-GlcNAc as the end product
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?
Man3GlcNAc2 + H2O
Man2GlcNAc2 + alpha-D-mannopyranose
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Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
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?
Man5-9GlcNAc + H2O
alpha-D-mannopyranose + ?
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low activity for Man5GlcNAc
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?
Man5GlcNAc2 + H2O
Man4GlcNAc2 + alpha-D-mannopyranose
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cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
mixture of three isomeric oligosaccharides
?
Man6 + H2O
alpha-D-mannose + ?
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derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI
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?
Man6GlcNAc2 + H2O
Man5GlcNAc2 + mannose
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slow reaction of the Golgi alpha-mannosidase in vitro
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?
Man9-5GlcNAc + 4 H2O
Man5-3GlcNAc + 4 alpha-D-mannopyranose
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different product composition for alpha-mannosidases IA, IB and II
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?
Man9GlcNAc + H2O
Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose
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Co2+ activated alpha-mannosidase
end products
?
Man9GlcNAc2 + 3 H2O
Man6GlcNAc2 + 3 mannose
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fast reaction of the Golgi alpha-mannosidase in vitro
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?
Man9GlcNAc2 + H2O
Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
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?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
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-
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?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
alpha-D-mannopyranose + ?
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?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + H2O
Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-4)anhydromannitol + anhydromannitol + alpha-D-mannose
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equal amounts, but only traces of Manalpha(1-4)-anhydromannitol
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
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?
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
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-
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?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
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highly active on alpha(1-2) linked mannooligosaccharides
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?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
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?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + N-acetyl-D-glucosamine
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?
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
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-
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?
Manalpha(1-4)Manbeta(1-4)Man + H2O
alpha-D-mannopyranose + Manbeta(1-4)Man
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-
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?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
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i.e. GPIV
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?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
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-
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?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + N-acetyl-D-glucosamine
-
-
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
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4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
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?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
-
-
-
?
mannosyl rhamnose + H2O
alpha-D-mannopyranose + rhamnose
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part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium
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?
mannosyl(5)-N-acetyl-glucosamine-pyridylamino + H2O
mannosyl(4)-N-acetyl-glucosamine-pyridylamino + mannosyl(3)-N-acetyl-glucosamine-pyridylamino
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occurrence of mannosyl(4)-N-acetyl-glucosamine-pyridylamino (32%) and mannosyl(3)-N-acetyl-glucosamine-pyridylamino (8.1%) are observed only in the presence of Co2+
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?
mannosyl(9)-N-acetyl-glucosamine + 4 H2O
mannosyl(5)-N-acetyl-glucosamine + 4 alpha-D-mannose
in presence of Co2+
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-
?
mannosyl(9)-N-acetyl-glucosamine + H2O
mannosyl(8)-N-acetyl-glucosamine + alpha-D-mannose
absence of Co2+
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-
?
methyl-2,4,6-tri-O-methyl-D-mannopyranoside + H2O
2,4,6-tri-O-methyl-D-mannopyranose + methanol
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-
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?
methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
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-
-
-
?
methyl-2-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
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very low activity
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?
methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
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-
-
?
methyl-alpha-D-mannnopyranose + H2O
alpha-D-mannopyranose + methanol
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-
-