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Literature summary for 3.2.1.24 extracted from

  • Suzuki, T.; Hara, I.; Nakano, M.; Shigeta, M.; Nakagawa, T.; Kondo, A.; Funakoshi, Y.; Taniguchi, N.
    Man2C1, an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol (2006), Biochem. J., 400, 33-41.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
deoxymannojirimycin less effective compared with swainsonine, concentration of 100 microM Homo sapiens
kifunensine little inhibitory effect, concentration of 10 microM Homo sapiens
additional information down-regulation of Man2C1 activity by a small interfering RNA drastically change amount and structure of oligosaccharides accumulating in the cytosol, demonstrating that Man2C1 is involved in free oligosaccharide processing in the cytosol Homo sapiens
small interfering RNA siRNA designed to specifically inhibit Man2C1 gene expression, alpha-mannosidase activity is compromised in siRNA-treated cells. Determination of alteration of siRNA treatment, glycans recovered from membrane glycoproteins are fluorescence-labelled and analysed by HPLC Homo sapiens
swainsonine concentration of 100 microM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol examination of subcellular localization of Man2C1, using FLAG-tagging at the N-terminus for detection Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhances acivity, alpha-mannosidase in absence of Co2+ only converts mannosyl(9)-N-acetyl-glucosamine into mannosyl(8)-N-acetyl-glucosamine, in presence of Co2+, alpha-mannosidase yields mannosyl(5)-N-acetyl-glucosamine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NTJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
FLAG–tagged Man2C1 from cytosolic fraction of HEK-293 cells, analysed by silver staining Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-mannoside + H2O pNP-alpha-Man, used for enzyme activity test Homo sapiens 4-nitrophenol + alpha-D-mannose
-
 ?
mannosyl(5)-N-acetyl-glucosamine-pyridylamino + H2O
-
 Homo sapiens mannosyl(4)-N-acetyl-glucosamine-pyridylamino + mannosyl(3)-N-acetyl-glucosamine-pyridylamino occurrence of mannosyl(4)-N-acetyl-glucosamine-pyridylamino (32%) and mannosyl(3)-N-acetyl-glucosamine-pyridylamino (8.1%) are observed only in the presence of Co2+ ?
mannosyl(9)-N-acetyl-glucosamine + 4 H2O in presence of Co2+ Homo sapiens mannosyl(5)-N-acetyl-glucosamine + 4 alpha-D-mannose
-
 ?
mannosyl(9)-N-acetyl-glucosamine + H2O absence of Co2+ Homo sapiens mannosyl(8)-N-acetyl-glucosamine + alpha-D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
alpha-mannosidase
-
 Homo sapiens
ER-mannosidase II
-
 Homo sapiens
Man2C1
-
 Homo sapiens
Man2C1 alpha-mannosidase
-
 Homo sapiens
neutral/cytosol mannosidase
-
 Homo sapiens