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Literature summary for 3.2.1.24 extracted from

  • Beccari, T.; Appolloni, M.G.; Costanzi, E.; Stinchi, S.; Stirling, J.L.; Della Fazia, M.A.; Servillo, G.; Viola, M.P.; Orlacchio, A.
    Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA (1997), Biochem. J., 327, 45-49.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 isoform B, gel filtration Mus musculus
290000
-
 isoform A, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-mannopyranoside + H2O Mus musculus
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O09159 isoforms A and B
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoforms A and B different in sialic acid content Mus musculus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
epididymis only isoform B present Mus musculus
-
kidney
-
 Mus musculus
-
liver only isoform B present Mus musculus
-
spleen
-
 Mus musculus
-
testis
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Mus musculus 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
alpha-D-mannopyranoside + H2O
-
 Mus musculus alpha-D-mannopyranose + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to 1 h Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 isoforms A and B Mus musculus