Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxy-mannojirimycin | complete inhibition | Solanum lycopersicum | |
Co2+ | - |
Solanum lycopersicum | |
Cu2+ | - |
Solanum lycopersicum | |
swainsonine | complete inhibition | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
4-nitrophenyl-alpha-D-mannopyranoside | pH 5.0 | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE | Solanum lycopersicum |
70000 | - |
2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE | Solanum lycopersicum |
230000 | - |
gel filtration | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from amture red fruit | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Solanum lycopersicum | 4-nitrophenol + alpha-D-mannopyranose | - |
? | |
additional information | enzyme hydrolyzes alpha-mannosidase linkages from both high-mannose type and plant complex type N-glycan, but prefers a truncated plant complex type structure to high-mannose type N-glycans bearing alpha-(1,2)-mannosyl residues | Solanum lycopersicum | ? | - |
? | |
additional information | enzyme is active toward both high-mannose type and truncated plant complex type N-glycans. It prefers truncated plant complex type N-glycans | Solanum lycopersicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 70000, L-subunit, + 2 * 47000, S-subunit, SDS-PAGE | Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
substrate 4-nitrophenyl-alpha-D-mannopyranoside | Solanum lycopersicum |
6 | - |
substrate Man8GlcNAc2-pyridylaminoside | Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
23% of maximum activity | Solanum lycopersicum |