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Literature summary for 3.2.1.24 extracted from

  • Moremen, K.W.; Touster, O.; Robbins, P.W.
    Novel purification of the catalytic domain of golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme (1991), J. Biol. Chem., 266, 16876-16885.
    View publication on PubMed
Search for more literature for 3.2.1.24

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane from Golgi-enriched membrane fraction Rattus sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 2 * 100000, reducing SDS-PAGE, catalytic subunit Rattus sp.
124000
-
 2 * 124000, SDS-PAGE Rattus sp.
185000 200000 chymotrypsin-cleaved catalytic subunit, non-reducing SDS-PAGE, gel filtration Rattus sp.
285000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcNAcMan5GlcNAc + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
catalytic subunit, to homogeneity Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver isolation of the catalytic domain by chymotrypsin digestion Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAcMan5GlcNAc + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 124000, SDS-PAGE Rattus sp.
dimer 2 * 100000, reducing SDS-PAGE, catalytic subunit Rattus sp.

Synonyms

Synonyms Comment Organism
Golgi alpha-mannosidase II
-
 Rattus sp.