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Literature summary for 3.2.1.24 extracted from

  • Okumura, T.; Yamashina, I.
    Further purification and characterization of alpha-mannosidase from hog kidney (1973), J. Biochem., 73, 131-138.
    View publication on PubMed
Search for more literature for 3.2.1.24

General Stability

General Stability Organism
very stable if solution is kept frozen Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
p-nitrophenyl-alpha-D-mannopyranoside substrate inhibition greater than 2 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE, non-denaturating electrophoresis Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-3)GlcNAc + H2O Sus scrofa
-
 alpha-D-mannopyranose + N-acetyl-D-glucosamine
-
 ?
Manalpha(1-4)Manbeta(1-4)Man + H2O Sus scrofa
-
 alpha-D-mannopyranose + Manbeta(1-4)Man
-
 ?
Manalpha(1-6)GlcNAc + H2O Sus scrofa
-
 alpha-mannopyranose + N-acetyl-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 8% mannose, 3.3% glucosamine Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.1
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-3)GlcNAc + H2O
-
 Sus scrofa alpha-D-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
-
 ?
Manalpha(1-3)GlcNAc + H2O
-
 Sus scrofa alpha-D-mannopyranose + N-acetyl-D-glucosamine
-
 ?
Manalpha(1-4)Manbeta(1-4)Man + H2O
-
 Sus scrofa alpha-D-mannopyranose + Manbeta(1-4)Man
-
 ?
Manalpha(1-6)GlcNAc + H2O
-
 Sus scrofa alpha-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
-
 ?
Manalpha(1-6)GlcNAc + H2O
-
 Sus scrofa alpha-mannopyranose + N-acetyl-D-glucosamine
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Sus scrofa p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Sus scrofa