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Literature summary for 3.2.1.24 extracted from
- Characterization of a-mannosidase from Dolichos lablab seeds: Partial amino acid sequencing and N-glycan analysis (2013), Protein Expr. Purif., 89, 7-15.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Lablab purpureus |  |
| swainsonine | - |
Lablab purpureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.48 | - |
4-nitrophenyl alpha-D-mannopyranoside | pH 5.0, 37°C | Lablab purpureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
2 * 116000, SDS-PAGE | Lablab purpureus |
| 230000 | - |
gel filtration | Lablab purpureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lablab purpureus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | partial site-specific glycosylation analysis, paucimannosidic type N-glycan | Lablab purpureus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, anion exchange, galactose Sepharose, phenyl Sepharose, gel permeation and Con A Sepharose chromatography | Lablab purpureus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Lablab purpureus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannopyranoside + H2O | - |
Lablab purpureus | 4-nitrophenol + D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 116000, SDS-PAGE | Lablab purpureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acidic alpha-mannosidase | belongs to glycosyl hydrolase 38 | Lablab purpureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
up to, pH 5.0 | Lablab purpureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
acetate buffer | Lablab purpureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Lablab purpureus |
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