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Literature summary for 3.2.1.24 extracted from
- Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase (1996), J. Biol. Chem., 271, 28348-28358.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| swainsonine | IC50: 0.00011 mM | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-mannosidosis fibroblasts + H2O | Homo sapiens | - |
Man7-2GlcNAc + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.29 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6 pyridylamino-Man9GlcNAc2 + H2O | - |
Homo sapiens | pyridylamino-Man8GlcNAc2 + pyridylamino-Man7GlcNAc2 + pyridylamino-Man6GlcNAc2 + pyridylamino-Man5GlcNAc2 + pyridylamino-Man4GlcNAc2 + pyridylamino-Man3GlcNAc2 + 21 alpha-D-mannopyranose | - |
? | |
| alpha-D-mannosidosis fibroblasts + H2O | - |
Homo sapiens | Man7-2GlcNAc + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Homo sapiens | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
| pyridylamino-Man5GlcNAc + 3 H2O | - |
Homo sapiens | pyridylamino-Man2GlcNA + alpha-D-mannopyranose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 6 | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
IC50: 0.00011 mM | Homo sapiens | swainsonine | |
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