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Literature summary for 3.2.1.24 extracted from
- Purification of alpha-mannosidase from hog kidney and its action on glycopeptides (1970), J. Biochem., 68, 561-571.
General Stability
| General Stability | Organism |
|---|---|
| addition of Zn2+ stabilizes activity | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-nitrophenyl-alpha-D-mannopyranoside | substrate inhibition greater than 2 mM | Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | Sus scrofa | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
| glycopeptide + H2O | Sus scrofa | stem bromelain | alpha-D-mannopyranose + ? | - |
? | |
| TAKA-amylase A + H2O | Sus scrofa | - |
alpha-D-mannopyranose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.9 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | from ovalbumin | Sus scrofa | alpha-D-mannopyranose + ? | - |
? | |
| glycopeptide + H2O | stem bromelain | Sus scrofa | alpha-D-mannopyranose + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Sus scrofa | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
| TAKA-amylase A + H2O | - |
Sus scrofa | alpha-D-mannopyranose + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable within 15 min | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Sus scrofa |
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