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Literature summary for 3.2.1.24 extracted from
- Purification and properties of a glycoprotein processing alpha-mannosidase from mung bean seedlings (1986), Plant Physiol., 81, 383-389.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | increasing activity up to 0.1% | Vigna radiata |  |
General Stability
| General Stability | Organism |
|---|---|
| quite stable in buffer containing 10% glycerol, v/v | Vigna radiata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimicin | 50% inhibition at 0.05 mM | Vigna radiata | |
| D-mannosylamine | - |
Vigna radiata | |
| EDTA | 60-75% inhibition at 4 mM, activity restored by addition of Ca2+ | Vigna radiata | |
| Mg2+ | - |
Vigna radiata | |
| Mn2+ | - |
Vigna radiata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Vigna radiata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20-30% activation at 5 mM | Vigna radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man9GlcNAc + 4 H2O | Vigna radiata | hydrolyses alpha-1,2- and alpha-1,3-linkages | Man5GlcNAc + 4 alpha-D-mannopyranose | after 24 h incubation, specifically removes alpha-1,2-linked mannoses | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, affinity chromatography | Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Vigna radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man9GlcNAc + 4 H2O | hydrolyses alpha-1,2- and alpha-1,3-linkages | Vigna radiata | Man5GlcNAc + 4 alpha-D-mannopyranose | after 24 h incubation, specifically removes alpha-1,2-linked mannoses | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Vigna radiata |
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