BRENDA - Enzyme Database
show all sequences of 3.2.1.24

Class II alpha-mannosidase from Aspergillus fischeri: energetics of catalysis and inhibition

Shashidhara, K.S.; Gaikwad, S.M.; Int. J. Biol. Macromol. 44, 112-115 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1-deoxymannojirimycin
binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
swainsonine
binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0073
-
4-methylumbelliferyl-alpha-D-mannopyranoside
pH 6.5, 50°C
Aspergillus fischeri
0.00731
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3
Aspergillus fischeri
0.0826
-
4-nitrophenyl-alpha-D-mannopyranoside
pH 6.5, 50°C
Aspergillus fischeri
0.08265
-
4-nitrophenyl-alpha-D-mannopyranoside
-
Aspergillus fischeri
0.839
-
Manalpha(1-3)Man
pH 6.5, 50°C
Aspergillus fischeri
0.839
-
Man-alpha(1-3)Man
pH 6.5, 50°C, 20 min
Aspergillus fischeri
1.116
-
Manalpha(1-2)Man
pH 6.5, 50°C
Aspergillus fischeri
1.455
-
Man-alpha(1-6)Man
pH 6.5, 50°C; pH 6.5, 50°C, 20 min
Aspergillus fischeri
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus fischeri
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Aspergillus fischeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3
698317
Aspergillus fischeri
4-methylumbelliferone + alpha-D-mannose
-
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-methylumbelliferone + alpha-D-mannopyranose
-
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-nitrophenol + D-mannose
-
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-nitrophenyl + alpha-D-mannose
-
-
-
?
Man-alpha(1-3)Man + H2O
pH 6.5, 50°C, 20 min
698317
Aspergillus fischeri
alpha-D-mannose
-
-
-
?
Man-alpha(1-6)Man + H2O
pH 6.5, 50°C, 20 min
698317
Aspergillus fischeri
alpha-D-mannose
-
-
-
?
Manalpha(1-2)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Manalpha(1-3)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Manalpha(1-6)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
class II alpha-mannosidase
-
Aspergillus fischeri
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Aspergillus fischeri
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
15 min
Aspergillus fischeri
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
fully stable, no inactivation, no denaturation
Aspergillus fischeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Aspergillus fischeri
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.101
-
swainsonine
low binding up to 40°C, increased binding at 50°C; pH 6.5, 50°C
Aspergillus fischeri
0.286
-
1-deoxymannojirimycin
low binding up to 40°C, increased binding at 50°C; pH 6.5, 50°C
Aspergillus fischeri
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.11
-
low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
swainsonine
0.284
-
low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
1-deoxymannojirimycin
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.11
-
low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
swainsonine
0.284
-
low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
1-deoxymannojirimycin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-deoxymannojirimycin
binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
swainsonine
binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C
Aspergillus fischeri
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.101
-
swainsonine
low binding up to 40°C, increased binding at 50°C; pH 6.5, 50°C
Aspergillus fischeri
0.286
-
1-deoxymannojirimycin
low binding up to 40°C, increased binding at 50°C; pH 6.5, 50°C
Aspergillus fischeri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0073
-
4-methylumbelliferyl-alpha-D-mannopyranoside
pH 6.5, 50°C
Aspergillus fischeri
0.00731
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3
Aspergillus fischeri
0.0826
-
4-nitrophenyl-alpha-D-mannopyranoside
pH 6.5, 50°C
Aspergillus fischeri
0.08265
-
4-nitrophenyl-alpha-D-mannopyranoside
-
Aspergillus fischeri
0.839
-
Manalpha(1-3)Man
pH 6.5, 50°C
Aspergillus fischeri
0.839
-
Man-alpha(1-3)Man
pH 6.5, 50°C, 20 min
Aspergillus fischeri
1.116
-
Manalpha(1-2)Man
pH 6.5, 50°C
Aspergillus fischeri
1.455
-
Man-alpha(1-6)Man
pH 6.5, 50°C; pH 6.5, 50°C, 20 min
Aspergillus fischeri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus fischeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3
698317
Aspergillus fischeri
4-methylumbelliferone + alpha-D-mannose
-
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-methylumbelliferone + alpha-D-mannopyranose
-
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-nitrophenol + D-mannose
-
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
698317
Aspergillus fischeri
4-nitrophenyl + alpha-D-mannose
-
-
-
?
Man-alpha(1-3)Man + H2O
pH 6.5, 50°C, 20 min
698317
Aspergillus fischeri
alpha-D-mannose
-
-
-
?
Man-alpha(1-6)Man + H2O
pH 6.5, 50°C, 20 min
698317
Aspergillus fischeri
alpha-D-mannose
-
-
-
?
Manalpha(1-2)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Manalpha(1-3)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Manalpha(1-6)Man + H2O
-
698317
Aspergillus fischeri
D-mannose
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Aspergillus fischeri
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
15 min
Aspergillus fischeri
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
fully stable, no inactivation, no denaturation
Aspergillus fischeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Aspergillus fischeri
Other publictions for EC 3.2.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751259
Lund
Comprehensive long-term effic ...
Homo sapiens
J. Inherit. Metab. Dis.
41
1225-1233
2018
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-
751574
Harmatz
Enzyme replacement therapy wi ...
Homo sapiens
Mol. Genet. Metab.
124
152-160
2018
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1
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1
1
-
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-
750617
Paciotti
Lysosomal alpha-mannosidase a ...
Homo sapiens
Front. Biosci.
22
157-167
2017
-
-
-
-
2
-
2
-
3
1
2
-
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3
-
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1
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4
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1
2
3
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1
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1
2
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1
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4
-
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1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
729186
Hansen
Mutational analysis of divalen ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
24
2032-2039
2015
-
-
-
-
7
-
1
12
-
4
-
-
-
5
-
-
1
-
-
-
-
-
2
-
3
1
1
-
14
1
1
1
-
-
-
-
-
-
-
-
-
7
-
-
1
-
12
-
4
-
-
-
-
-
1
-
-
-
-
2
-
1
1
-
14
1
1
1
-
-
-
-
-
20
20
750016
Shah
3D-QSAR and molecular modelin ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Bioorg. Chem.
59
91-96
2015
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
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728977
Kashyap
Deoxysugars as antituberculars ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Antimicrob. Agents Chemother.
58
3530-3532
2014
-
-
-
-
-
-
4
-
-
-
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-
172
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4
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4
4
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-
-
-
-
-
-
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-
-
-
-
723704
Gnanesh Kumar
Characterization of a-mannosid ...
Lablab purpureus
Protein Expr. Purif.
89
7-15
2013
-
-
-
-
-
-
2
1
-
-
2
-
-
1
-
1
1
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1
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1
1
1
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1
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1
1
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2
-
1
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-
2
-
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1
1
-
1
-
-
1
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
730144
Borgwardt
Enzyme replacement therapy for ...
Homo sapiens
J. Inherit. Metab. Dis.
36
1015-1024
2013
-
-
-
-
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2
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1
1
-
-
-
724357
Nielsen
Metal-ion dependent catalytic ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochemistry
51
8039-8046
2012
-
-
1
-
-
-
3
11
-
4
-
-
-
5
-
-
1
-
-
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-
10
-
2
1
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-
11
1
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1
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3
-
11
-
4
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1
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-
-
10
-
1
-
-
11
1
-
-
-
-
-
-
-
18
18
724934
Koerdt
Complementation of Sulfolobus ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
16
115-125
2012
-
-
1
-
-
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5
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1
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1
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1
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1
1
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-
729814
Riise Stensland
Identification of 83 novel alp ...
Homo sapiens
Hum. Mutat.
33
511-520
2012
-
-
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-
34
-
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1
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3
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1
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1
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34
-
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1
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1
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-
730990
Retamal
Alpha-mannosidase activity in ...
Equus caballus
Theriogenology
78
252-262
2012
-
-
-
-
-
-
1
1
1
3
1
-
-
1
-
-
-
-
-
2
-
1
1
-
4
-
-
1
-
1
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1
-
1
1
3
1
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2
-
1
1
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-
1
-
1
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-
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-
1
1
-
-
-
715596
Gregg
Analysis of a new family of wi ...
Clostridium perfringens, Streptococcus pneumoniae
J. Biol. Chem.
286
15586-15596
2011
-
-
2
1
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2
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2
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4
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2
4
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2
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2
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1
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2
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4
-
4
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2
-
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2
2
715769
Ghosh
The N-glycan processing enzyme ...
Capsicum annuum
J. Exp. Bot.
62
571-582
2011
-
-
1
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1
1
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3
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1
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1
1
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1
1
1
2
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1
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1
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1
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1
1
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3
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1
1
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1
1
2
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1
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1
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1
1
1
1
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-
710392
Suits
Structure and kinetic investig ...
Streptococcus pyogenes M1 GAS
PLoS ONE
5
e9006
2010
-
-
1
1
-
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1
2
1
1
-
2
-
4
-
-
1
-
-
-
-
-
7
1
6
1
-
-
2
1
-
-
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1
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The molecular characterization ...
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1
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1
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6
2
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1
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13
1
2
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1
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Molecular characterization of ...
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696847
Hossain
alpha-mannosidase involved in ...
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1
1
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2
1
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698317
Shashidhara
Class II alpha-mannosidase fro ...
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1
1
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Cerebrospinal fluid beta-gluco ...
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700483
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Inhibition of alpha-mannosidas ...
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Racanicchi
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Kuntz
Structural analysis of Golgi a ...
Drosophila melanogaster
Biochemistry
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Fiaux
Functionalized pyrrolidine inh ...
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678423
Kestwal
Characterization of alpha-mann ...
Erythrina variegata
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678607
Sivapriya
Conformationally locked thiosu ...
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1
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679284
Pittis
Funtional characterization of ...
Homo sapiens
Clin. Chim. Acta
375
136-139
2007
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679943
Jacobson
Glycolytic and chitinolytic ac ...
Phlebotomus papatasi
Folia Parasitol.
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301-309
2007
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663538
Nakajima
Crystallization and preliminar ...
Thermotoga maritima
Acta Crystallogr. Sect. F
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104-105
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2
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1
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1
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1
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665065
Faid
Site-specific glycosylation an ...
Bos taurus
Glycobiology
16
440-461
2006
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678045
Suzuki
Man2C1, an alpha-mannosidase, ...
Homo sapiens
Biochem. J.
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33-41
2006
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Angelov
Molecular and biochemical char ...
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664603
Siriwardena
Potent and selective inhibitio ...
Homo sapiens
ChemBioChem
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Fiaux
Functionalized pyrrolidines in ...
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655180
Nishio
alpha-Mannosidase-catalyzed sy ...
Canavalia ensiformis
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655689
Mora-Montes
Hydrolysis of Man9GlcNAc2 and ...
Candida albicans
Glycobiology
14
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3
1
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Tempel
Structure of mouse Golgi alpha ...
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Numao
Insights into the mechanism of ...
Drosophila melanogaster
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278
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Nakajima
Unique metal dependency of cyt ...
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Heikinheimo
The structure of bovine lysoso ...
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Desmet
Novel tools for the study of c ...
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Yamagishi
Co(II)-regulated substrate spe ...
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Structure of Golgi alpha-manno ...
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alpha-D-Mannosidase properties ...
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Purification and characterizat ...
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Vazquez-Reyna
Purification and biochemical c ...
Candida albicans
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2
2
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1
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Jin
Purification and properties of ...
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1
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Characterization of a soluble ...
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135959
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Purification, crystallization ...
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Vandersall-Nairn
Cloning, expression, purificat ...
Trypanosoma cruzi
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646610
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Separation, characterization, ...
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Beccari
Lysosomal alpha-mannosidases o ...
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135962
Merkle
Cloning, expression, purificat ...
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Yamashiro
Purification and characterizat ...
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Cloning, expression, purificat ...
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Gaikwald
Purification and characterizat ...
Aspergillus sp.
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Schneikert
Characterization of a novel mo ...
Mus musculus
Glycobiology
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1994
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Soluble forms of alpha-D-manno ...
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Tulsiani
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Purification and characterizat ...
Rattus sp.
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Vazquez-Reyna
Biosynthesis of glycoproteins ...
Candida albicans
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1
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135970
Swanson
Identification and characteriz ...
Trypanosoma cruzi
Glycobiology
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1992
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1
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1
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1
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2
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1
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1
1
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2
1
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1
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135971
Moremen
Novel purification of the cata ...
Rattus sp.
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1
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Bonay
Purification and characterizat ...
Rattus sp.
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7
2
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1
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1
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1
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1
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Bischoff
Isolation, characterization, a ...
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Schutzbach
Calcium ion activation of rabb ...
Oryctolagus cuniculus
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1990
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2
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1
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1
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1
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6
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1
2
1
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2
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1
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135975
Lopez-Valbuena
-
Purification and partial chara ...
Helianthus annuus
Plant Sci.
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8
2
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1
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8
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1
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1
3
1
1
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1
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1
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135976
Gunnarsson
-
Studies of the reversed alpha- ...
Canavalia ensiformis
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1
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3
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135977
Tanimoto
-
Induction and purification of ...
Aspergillus oryzae
Agric. Biol. Chem.
53
1083-1088
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1
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135978
Einhoff
The alpha-mannosidase from Can ...
Canavalia ensiformis
Biol. Chem. Hoppe-Seyler
369
165-169
1988
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1
-
-
5
-
-
1
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
2
-
-
-
1
1
-
5
-
-
1
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
135979
Yoshihisa
Solubilization and purificatio ...
Saccharomyces cerevisiae
J. Biol. Chem.
263
5158-5163
1988
-
-
-
-
-
-
1
2
2
-
1
-
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
-
1
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135980
Niyogi
-
An immunological distinct form ...
Canavalia ensiformis
Phytochemistry
27
2737-2741
1988
-
-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
1
-
-
5
3
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
1
-
5
3
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135982
Vega
-
Partial characterization of al ...
Yarrowia lipolytica
J. Basic Microbiol.
28
371-379
1988
-
-
-
-
-
-
1
1
1
1
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
135983
Mathur
Co2+-mediated time- and temper ...
Macaca radiata
Biochem. J.
253
677-685
1988
-
-
-
-
-
1
2
-
-
1
1
-
-
2
-
-
1
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
1
-
-
1
1
-
-
-
-
1
-
2
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135984
Tulsiani
Rat epididymal alpha-D-mannosi ...
Rattus sp.
Arch. Biochem. Biophys.
267
60-68
1988
-
-
-
-
-
-
-
1
-
-
-
2
-
3
-
1
1
-
-
2
1
1
3
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
1
-
2
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135985
Zacharova
-
Alpha-mannosidase from Rhodoco ...
Rhodococcus erythropolis
Methods Enzymol.
160
620-626
1988
-
-
-
-
-
1
10
1
1
2
1
1
-
1
-
1
1
-
-
1
1
1
1
1
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
10
-
1
1
2
1
1
-
-
1
1
-
1
1
1
1
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
135988
Nakagawa
-
Purification and characterizat ...
Citrullus lanatus
Agric. Biol. Chem.
52
2223-2230
1988
-
-
-
-
-
1
1
1
-
-
1
1
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
135986
Conti
-
Alpha-D-mannosidase from the a ...
Triticum aestivum
Phytochemistry
26
2909-2912
1987
-
-
-
-
-
-
3
-
-
1
1
-
-
1
-
1
1
-
-
1
1
-
1
-
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
1
1
-
1
1
-
1
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
135987
Waln
-
Partial purification and chara ...
Prunus serotina
Plant Sci.
53
1-10
1987
-
-
-
-
-
-
4
2
-
1
1
-
-
1
-
1
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
1
1
-
-
-
1
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135989
Cheng
Purification and comparison of ...
Homo sapiens
Biochem. J.
233
65-72
1986
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
1
1
-
-
1
2
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
1
-
1
2
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135991
Shigematsu
-
Molecular and enzymic properti ...
Aspergillus phoenicis
Curr. Microbiol.
13
43-46
1986
-
-
-
-
-
-
8
1
-
-
-
2
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135992
Szumilo
Purification and properties of ...
Vigna radiata
Plant Physiol.
81
383-389
1986
1
-
-
-
-
1
5
-
1
1
-
1
-
3
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
5
-
-
1
1
-
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
586942
Amano
Purification and characterizat ...
Aspergillus phoenicis
J. Biochem.
99
1645-1654
1986
-
-
-
-
-
-
-
1
-
1
-
2
-
3
-
-
1
-
-
1
-
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
646577
Tanimoto
Improved purification of Asper ...
Aspergillus oryzae
J. Biochem.
99
601-604
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135993
Tulsiani
Characterization of a novel al ...
Rattus sp.
J. Biol. Chem.
260
13081-13087
1985
-
-
-
-
-
-
-
-
1
-
-
3
-
2
-
-
1
-
-
2
-
-
4
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
2
-
-
4
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
136024
Mathur R.; Balasubramanian
Cobalt-ion chelate affinity ch ...
Macaca radiata
Biochem. J.
222
261-264
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135995
Shigeta
Purification and characterizat ...
Styela plicata
J. Biochem.
94
1827-1832
1983
-
-
-
-
-
-
5
-
-
1
2
2
-
3
-
-
1
-
-
1
1
1
6
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
2
2
-
-
-
1
-
1
1
1
6
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135996
Van der Wilden
Characterization of the isoenz ...
Phaseolus vulgaris
Plant Physiol.
71
82-87
1983
-
-
-
-
-
-
-
-
7
1
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
1
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
135997
Bischoff
Evidence for an alpha-mannosid ...
Rattus sp.
J. Biol. Chem.
258
7907-7910
1983
-
-
-
-
-
1
-
1
3
-
-
2
-
2
-
-
1
-
-
3
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
3
-
-
2
-
-
-
1
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135998
Tulsiani
alpha-D-Mannosidases of rat li ...
Rattus sp.
J. Biol. Chem.
257
3660-3668
1982
-
-
-
-
-
-
7
-
2
2
-
1
-
2
-
-
1
-
-
1
-
-
2
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
2
2
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
135999
Plant
-
alpha-D-Mannosidase and alpha- ...
Lupinus angustifolius
Phytochemistry
21
985-989
1982
-
-
-
-
-
-
6
2
1
1
1
1
-
1
-
-
1
-
-
1
2
-
3
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
1
1
1
1
-
-
-
1
-
1
2
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
136000
Yamamoto
Purification and characterizat ...
Aspergillus oryzae
J. Biochem.
91
1971-1979
1982
2
-
-
-
-
-
16
2
-
2
1
5
-
4
-
-
1
-
-
1
-
1
12
-
-
1
-
1
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
16
-
2
-
2
1
5
-
-
-
1
-
1
-
1
12
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-
136001
Ichishima
Purification of an acidic alph ...
Aspergillus phoenicis
Biochim. Biophys. Acta
658
45-53
1981
-
-
-
-
-
-
-
1
1
-
1
2
-
3
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
2
-
-
-
1
-
2
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136002
Forsee
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
256
6577-6582
1981
3
-
-
-
-
-
3
-
1
-
-
4
-
2
-
-
1
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
3
3
-
1
-
-
4
-
-
-
1
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136003
Curdel
Purification and properties of ...
Medicago sativa
Biochem. J.
185
455-462
1980
-
-
-
-
-
-
8
1
-
-
5
-
-
3
-
-
1
-
-
2
1
-
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
-
5
-
-
-
-
1
-
2
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
136004
Poenaru
alpha-Mannosidase in human red ...
Homo sapiens
Biochim. Biophys. Acta
566
67-71
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136005
Hirani
The multiple forms of alpha-D- ...
Homo sapiens
Biochem. J.
179
583-592
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136006
Opheim
Lysosomal alpha-D-mannosidase ...
Rattus sp.
J. Biol. Chem.
253
1017-1023
1978
-
-
-
-
-
-
2
1
3
-
1
8
-
2
-
-
1
-
-
2
-
1
11
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
3
-
1
8
-
-
-
1
-
2
-
1
11
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136007
Opheim
alpha-D-Mannosidase from rat l ...
Rattus sp.
Methods Enzymol.
50
494-500
1978
-
-
-
-
-
1
3
1
2
-
1
5
-
2
1
1
1
-
-
2
-
1
8
-
1
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
3
1
1
2
-
1
5
-
1
1
1
-
2
-
1
8
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
136008
Opheim
alpha-D-Mannosidase of Sacchar ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
524
121-130
1978
-
-
-
-
-
-
2
1
-
-
-
-
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136009
Augustin
-
Properties of extracellular al ...
Aspergillus flavus
Collect. Czech. Chem. Commun.
43
2812-2816
1978
1
-
-
-
-
-
5
1
1
-
-
-
-
1
-
-
1
-
-
1
1
1
1
-
-
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
1
1
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
136023
Ram
alpha-D-Mannosidase from golgi ...
Rattus sp.
Methods Enzymol.
50
500-505
1978
6
-
-
-
-
-
4
1
2
2
1
2
-
2
-
1
1
-
-
2
1
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
-
-
-
-
-
4
-
1
2
2
1
2
-
-
1
1
-
2
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136010
Katohda
-
Purification and some properti ...
Acinetobacter sp.
Agric. Biol. Chem.
41
331-337
1977
-
-
-
-
-
-
-
-
1
-
-
4
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136011
Kaya
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
82
1443-1449
1977
1
-
-
-
-
-
3
4
-
3
1
3
-
2
-
-
1
-
-
1
1
1
6
-
-
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
3
-
4
-
3
1
3
-
-
-
1
-
1
1
1
6
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
136012
Paus
Alpha-mannosidase from Phaseol ...
Phaseolus vulgaris
Eur. J. Biochem.
73
155-161
1977
-
-
-
-
-
-
1
2
-
1
1
-
-
3
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
1
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136013
Tulsiani
Purification and characterizat ...
Rattus sp.
J. Biol. Chem.
252
3227-3233
1977
6
-
-
-
-
-
4
1
2
2
2
-
-
2
-
1
1
-
-
2
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
-
-
-
-
-
4
-
1
2
2
2
-
-
-
1
1
-
2
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136014
Wallace
alpha-D-Mannosidases in rat sk ...
Rattus sp.
Biochem. Soc. Trans.
5
231-233
1977
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
136015
Shepherd
alpha-D-Mannosidase. Preparati ...
Canavalia ensiformis
Biochim. Biophys. Acta
429
884-894
1976
-
-
-
-
-
1
1
-
-
-
3
1
-
1
-
1
1
-
1
1
1
-
2
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
3
1
-
-
1
1
1
1
1
-
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
136016
Hultberg
Neutral alpha-mannosidase acti ...
Homo sapiens
Biochim. Biophys. Acta
445
398-405
1976
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
-
-
-
-
2
-
-
2
-
3
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
136017
Shoup
Purification and characterizat ...
Rattus sp.
J. Biol. Chem.
251
3845-3852
1976
-
-
-
-
-
1
1
1
4
-
2
-
-
2
1
1
1
-
-
2
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
4
-
2
-
-
1
1
1
-
2
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
136018
Snaith
Characterization of jack-bean ...
Canavalia ensiformis
Biochem. J.
147
83-90
1975
-
-
-
-
-
-
2
-
-
1
1
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135994
Okumura
Further purification and chara ...
Sus scrofa
J. Biochem.
73
131-138
1973
-
-
-
-
-
1
1
1
-
-
1
3
-
1
-
1
1
-
-
1
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
3
-
-
1
1
-
1
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
136019
Kaya
Isolation and characterization ...
Saccharomyces cerevisiae
J. Biochem.
73
181-182
1973
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
-
3
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
136020
Matta
Glycosidases of Aspergillus ni ...
Aspergillus niger
J. Biol. Chem.
247
1780-1787
1972
-
-
-
-
-
-
12
2
-
-
-
6
-
1
-
-
1
-
-
1
-
-
11
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
12
-
2
-
-
-
6
-
-
-
1
-
1
-
-
11
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
136021
Swaminathan
Glycosidases of Aspergillus ni ...
Aspergillus niger
J. Biol. Chem.
247
1775-1779
1972
-
-
-
-
-
-
1
1
-
-
-
4
-
1
-
-
1
-
-
1
1
-
5
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
4
-
-
-
1
-
1
1
-
5
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
136022
Okumura
Purification of alpha-mannosid ...
Sus scrofa
J. Biochem.
68
561-571
1970
-
-
-
-
-
1
1
1
-
-
-
3
-
1
-
-
1
-
-
1
1
-
4
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
3
-
-
-
1
-
1
1
-
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
136025
Muramatsu
alpha-Mannosidase and beta-man ...
Turbo cornutus
J. Biochem.
62
700-709
1967
1
-
-
-
-
-
1
1
-
1
-
3
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
3
-
-
-
1
-
1
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
136026
Li
Presence of alpha-D-mannosidic ...
Canavalia ensiformis
J. Biol. Chem.
241
1010-1012
1966
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
1
-
-
1
1
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-