Cloned (Comment) | Organism |
---|---|
TM1851, expression in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
purified recombinant TM1851, sitting drop vapour diffusion method, optimal conditions: 0.001 ml of protein solution containing 5.3 mg/ml protein in 5 mM sodium phosphate and 150 mM NaCl, pH 6.8, mixed with an equal volume of reservoir solution containing 4% w/v PEG 6000, 50 mM sodium phosphate, pH 6.0, and 0.5 M NaCl, 1 day at 25°C, X-ray diffraction structure determination and preliminary analysis at 2.9 A resolution | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Thermotoga maritima | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | preferred divalent cation | Thermotoga maritima | |
Co2+ | preferred divalent cation | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermotoga maritima | the exo-type cytosolic class II enzyme cleaves off alpha-1,2-, alpha-1,3-, and alpha-1,6-mannose residues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant TM1851 from Escherichia coli by ion exchange and hydroxyapatite chromatography, and gel filtration | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
purified recombinant TM1851 | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the exo-type cytosolic class II enzyme cleaves off alpha-1,2-, alpha-1,3-, and alpha-1,6-mannose residues | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class II alpha-mannosidase | - |
Thermotoga maritima |
More | the enzyme belongs to the glycoside hydrolase family 38 | Thermotoga maritima |
TM1851 | - |
Thermotoga maritima |