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Literature summary for 3.2.1.24 extracted from

  • Zacharova, I.Y.; Tamm, V.Y.; Pavlova, I.N.
    Alpha-mannosidase from Rhodococcus erythropolis (1988), Methods Enzymol., 160, 620-626.
No PubMed abstract available

General Stability

General Stability Organism
increasing thermostability in the presence of 0.1-1 mM CaCl2 Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ moderate inhibition at 1 mM Rhodococcus erythropolis
Cu2+ strong inhibition at 1 mM Rhodococcus erythropolis
EDTA strong inhibition at 1 mM Rhodococcus erythropolis
Fe2+ nearly complete inhibition at 1 mM Rhodococcus erythropolis
Hg2+ complete inhibition at 1 mM Rhodococcus erythropolis
Mn2+ slight inhibition at 1 mM Rhodococcus erythropolis
Ni2+ moderate inhibition at 1 mM Rhodococcus erythropolis
p-chloromercuribenzenesulfonic acid complete inhibition at 1 mM Rhodococcus erythropolis
Pb2+ moderate inhibition at 1 mM Rhodococcus erythropolis
Zn2+ moderate inhibition at 1 mM Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
Mannan value in mg/ml Rhodococcus erythropolis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rhodococcus erythropolis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ moderate activation at 1 mM Rhodococcus erythropolis
Mg2+ moderate activation at 1 mM Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000 32000 gel filtration, sedimentation equilibrium centrifugation Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mannan + H2O Rhodococcus erythropolis yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues alpha-D-mannose + ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 15% carbohydrate content, mannose, glucose, galactose major components Rhodococcus erythropolis

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, isoelectric focusing Rhodococcus erythropolis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Rhodococcus erythropolis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13890
-
-
Rhodococcus erythropolis

Storage Stability

Storage Stability Organism
-20°C, 0.2 mg protein/ml, 1 year Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mannan + H2O yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues Rhodococcus erythropolis alpha-D-mannose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000-32000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
mannanase
-
Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rhodococcus erythropolis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
fully inactivated in 5 min Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
in acetate buffer Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
4.3 9
-
Rhodococcus erythropolis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8 8 stable for 5 h at 20°C Rhodococcus erythropolis