Literature summary for 3.2.1.24 extracted from
Zacharova, I.Y.; Tamm, V.Y.; Pavlova, I.N.
Alpha-mannosidase from Rhodococcus erythropolis (1988), Methods Enzymol., 160, 620-626.
No PubMed abstract available
General Stability
General Stability |
Organism |
increasing thermostability in the presence of 0.1-1 mM CaCl2 |
Rhodococcus erythropolis |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Ag+ |
moderate inhibition at 1 mM |
Rhodococcus erythropolis |
|
Cu2+ |
strong inhibition at 1 mM |
Rhodococcus erythropolis |
|
EDTA |
strong inhibition at 1 mM |
Rhodococcus erythropolis |
|
Fe2+ |
nearly complete inhibition at 1 mM |
Rhodococcus erythropolis |
|
Hg2+ |
complete inhibition at 1 mM |
Rhodococcus erythropolis |
|
Mn2+ |
slight inhibition at 1 mM |
Rhodococcus erythropolis |
|
Ni2+ |
moderate inhibition at 1 mM |
Rhodococcus erythropolis |
|
p-chloromercuribenzenesulfonic acid |
complete inhibition at 1 mM |
Rhodococcus erythropolis |
|
Pb2+ |
moderate inhibition at 1 mM |
Rhodococcus erythropolis |
|
Zn2+ |
moderate inhibition at 1 mM |
Rhodococcus erythropolis |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
Mannan |
value in mg/ml |
Rhodococcus erythropolis |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
extracellular |
- |
Rhodococcus erythropolis |
- |
- |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ca2+ |
moderate activation at 1 mM |
Rhodococcus erythropolis |
|
Mg2+ |
moderate activation at 1 mM |
Rhodococcus erythropolis |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
30000 |
32000 |
gel filtration, sedimentation equilibrium centrifugation |
Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
mannan + H2O |
Rhodococcus erythropolis |
yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues |
alpha-D-mannose + ? |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Rhodococcus erythropolis |
- |
- |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
15% carbohydrate content, mannose, glucose, galactose major components |
Rhodococcus erythropolis |
Purification (Commentary)
Purification (Comment) |
Organism |
to homogeneity, chromatography steps, isoelectric focusing |
Rhodococcus erythropolis |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
cell culture |
- |
Rhodococcus erythropolis |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
13890 |
- |
- |
Rhodococcus erythropolis |
Storage Stability
Storage Stability |
Organism |
-20°C, 0.2 mg protein/ml, 1 year |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
mannan + H2O |
yeast alpha-mannan, main product: alpha-D-mannose, hydrolysis of linear and branched mannans containing terminal alpha-1,2- and alpha-1,3-linked mannopyranosyl residues |
Rhodococcus erythropolis |
alpha-D-mannose + ? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 30000-32000, SDS-PAGE |
Rhodococcus erythropolis |
Synonyms
Synonyms |
Comment |
Organism |
mannanase |
- |
Rhodococcus erythropolis |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
45 |
- |
- |
Rhodococcus erythropolis |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
60 |
- |
fully inactivated in 5 min |
Rhodococcus erythropolis |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5.8 |
- |
in acetate buffer |
Rhodococcus erythropolis |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
4.3 |
9 |
- |
Rhodococcus erythropolis |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
4.8 |
8 |
stable for 5 h at 20°C |
Rhodococcus erythropolis |