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Literature summary for 3.2.1.24 extracted from

  • Amano, J.; Kobata, A.
    Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi (1986), J. Biochem., 99, 1645-1654.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13
-
Man3GlcNAc2
-
Aspergillus phoenicis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strong activation Aspergillus phoenicis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man3GlcNAc2 + H2O Aspergillus phoenicis Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed Man2GlcNAc2 + alpha-D-mannopyranose
-
?
Man5GlcNAc2 + H2O Aspergillus phoenicis cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages Man4GlcNAc2 + alpha-D-mannopyranose mixture of three isomeric oligosaccharides ?

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aspergillus phoenicis
-

Storage Stability

Storage Stability Organism
4°C or -20°C, 4 months Aspergillus phoenicis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man3GlcNAc2 + H2O Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed Aspergillus phoenicis Man2GlcNAc2 + alpha-D-mannopyranose
-
?
Man5GlcNAc2 + H2O cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages Aspergillus phoenicis Man4GlcNAc2 + alpha-D-mannopyranose mixture of three isomeric oligosaccharides ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus phoenicis