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Literature summary for 3.2.1.24 extracted from
- Purification and characterization of an alpha-mannosidase of sea-squirt (1983), J. Biochem., 94, 1827-1832.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Styela plicata |  |
| diisopropylfluorophosphate | 42% inhibition at 10 mM | Styela plicata | |
| EDTA | addition of Zn2+ restores inhibitory effect | Styela plicata | |
| Fe2+ | - |
Styela plicata | |
| Hg2+ | - |
Styela plicata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | enhanced activity at 0.3-1 mM | Styela plicata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
4 * 70000, SDS-PAGE | Styela plicata |
| 275000 | - |
gel filtration | Styela plicata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | Styela plicata | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
| mannan + H2O | Styela plicata | from baker's and sake yeast | alpha-D-mannose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Styela plicata | - |
sea squirt | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Styela plicata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Styela plicata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Styela plicata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 6.0, 50% glycerol, 3 months | Styela plicata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl-alpha-D-mannopyranoside + H2O | - |
Styela plicata | 1-naphthol + alpha-D-mannopyranose | - |
? | |
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Styela plicata | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| 6-bromo-2-naphthyl-alpha-D-mannopyranoside + H2O | - |
Styela plicata | 6-bromo-2-naphthol + alpha-D-mannopyranose | - |
? | |
| glycopeptide + H2O | from ovalbumin | Styela plicata | alpha-D-mannopyranose + ? | - |
? | |
| mannan + H2O | from baker's and sake yeast | Styela plicata | alpha-D-mannose + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Styela plicata | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 70000, SDS-PAGE | Styela plicata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.4 | - |
- |
Styela plicata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Styela plicata |
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