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Literature summary for 3.2.1.24 extracted from
- Purification and biochemical characterization of two soluble alpha-mannosidases I and II from Candida albicans (1999), Glycobiology, 9, 533-537.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | hydrolysis of p-nitrophenyl-alpha-D-mannopyranoside, slight stimulation for enzyme I at 0.025 mM, enzyme II not affected | Candida albicans |  |
| swainsonine | hydrolysis of p-nitrophenyl-alpha-D-mannopyranoside, slight stimulation for enzyme I at 0.025 mM, enzyme II not affected | Candida albicans | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimicin | hydrolysis of 4-methylumbelliferyl-alpha-D-mannnopyranoside, strong inhibition for enzymes I and II at 0.025 mM | Candida albicans | |
| Ca2+ | inhibitory for enzyme I above 5 mM, enzyme II not affected | Candida albicans | |
| Mn2+ | inhibitory for enzyme I above 5 mM, slightly inhibitory for enzyme II | Candida albicans | |
| swainsonine | hydrolysis of 4-umbelliferyl-alpha-D-mannopyranoside, strong inhibition for enzymes I and II at 0.025 mM | Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.86 | - |
p-nitrophenyl-alpha-D-mannopyranoside | enzyme II | Candida albicans | |
| 2.04 | - |
p-nitrophenyl-alpha-D-mannopyranoside | enzyme I | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Candida albicans | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation for enzyme II between 0.5 and 2.5 mM | Candida albicans | |
| Mg2+ | slight stimulation for enzyme II between 0.5 and 2.5 mM | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54300 | - |
enzyme I, SDS-PAGE | Candida albicans |
| 93300 | - |
enzyme II, SDS-PAGE | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man6 + H2O | Candida albicans | derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI | alpha-D-mannose + ? | - |
? | |
| mannan + H2O | Candida albicans | derived from wild type or mutant strains of Saccharomyces cerevisiae, different specific activities for isoforms EI and EII, depending on the type of alpha-D-linkages of mutant substrates, preference for cleavage of alpha-1,6- and alpha-1,3-linkages | alpha-D-mannose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
two isoforms, enzymes I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps, isoelectric focusing | Candida albicans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Candida albicans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
enzyme I with 4-methylumbelliferyl-mannopyranoside as substrate | Candida albicans |
| 0.09 | - |
enzyme II with 4-methylumbelliferyl-mannopyranoside as substrate | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Candida albicans | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| Man6 + H2O | derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI | Candida albicans | alpha-D-mannose + ? | - |
? | |
| mannan + H2O | derived from wild type or mutant strains of Saccharomyces cerevisiae, different specific activities for isoforms EI and EII, depending on the type of alpha-D-linkages of mutant substrates, preference for cleavage of alpha-1,6- and alpha-1,3-linkages | Candida albicans | alpha-D-mannose + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Candida albicans | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54300, SDS-PAGE, enzyme I, 1 * 93300, SDS-PAGE, enzyme II | Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
enzymes I and II | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
enzymes I and II | Candida albicans |
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