Information on EC 2.8.2.4 - estrone sulfotransferase and Organism(s) Homo sapiens

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The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.4
-
RECOMMENDED NAME
GeneOntology No.
estrone sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:estrone 3-sulfotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-06-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
estrogen sulfotransferase SULT1E1 regulates inflammatory response and lipid metabolism of human endothelial cells via proliferator-activated receptor gamma
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17beta-estradiol + 3'-phosphoadenylyl sulfate
adenosine 3',5'-bisphosphate + 17beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phopshoadenylyl sulfate + resveratrol
adenosine 3',5'-bisphosphate + resveratrol 3-O-sulfate + resveratrol 4'-O-sulfate + resveratrol 3,4'-O-disulfate
show the reaction diagram
-
i.e. 3,5,4'-trihydroxystilbene, a phytoestrogen with mixed estrogen agonist/antagonist properties developed as a chemopreventive agent, interaction of resveratrol with 17beta-estradiol, overview
three different sulfate products, product identification by NMR, overview
-
?
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + 17beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 2-methyl-17beta-estradiol
adenosine 3',5'-bisphosphate + 2-methyl-17beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-cyano-7-hydroxycoumarin
adenosine 3',5'-bisphosphate + 3-cyano-7-hydroxycoumaryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3alpha-hydroxytibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 3beta-hydroxytibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + apigenin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + chrysin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + epicatechin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estrogen
adenosine 3',5'-bisphosphate + estrogen 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estrone
adenosine 3',5'-bisphosphate + estrone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + ethinyl estradiol
adenosine 3',5'-bisphosphate + ethinyl estradiol 3-sulfate
show the reaction diagram
-
a synthetic oral contraceptive, low activity
-
-
?
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + quercetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + resveratrol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 17alpha ethinyl estradiol
adenosine 3',5'-bisphosphate + 17alpha ethinyl estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 17alpha-ethinylestradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A3
-
-
?
3'-phosphoadenylylsulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-OH-1,3,7,8-tetrachlorinated dibenzo-p-dioxine
adenosine 3',5'-bisphosphate + 2-OH-1,3,7,8-tetrachlorinated dibenzo-p-dioxine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-1,3,7,8-tetrachlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 2-OH-1,3,7,8-tetrachlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-3,7,8-trichlorinated dibenzo-p-dioxine
adenosine 3',5'-bisphosphate + 2-OH-3,7,8-trichlorinated dibenzo-p-dioxine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-4,2',4'-trichlorodiphenyl ether
adenosine 3',5'-bisphosphate + 2-OH-4,2',4'-trichlorodiphenyl ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-4,5,3',2',3',4'-tetrachlorated biphenyl
adenosine 3',5'-bisphosphate + 2-OH-4,5,3',4'-tetrachlorated biphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-6,7,8-trichlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 2-OH-6,7,8-trichlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-7,8-dichlorinated dibenzo-p-dioxine
adenosine 3',5'-bisphosphate + 2-OH-7,8-dichlorinated dibenzo-p-dioxine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-OH-7,8-dichlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 2-OH-7,8-dichlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3,5,3',5'-tetrabromo-4,4'isopropylidendiphenol
adenosine 3',5'-bisphosphate + 3,5,3',5'-tetrabromo-4,4'isopropylidendiphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3,5,3',5'-tetrachloro-4,4'isopropylidendiphenol
adenosine 3',5'-bisphosphate + 3,5,3',5'- tetrachloro-4,4'isopropylidendiphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-OH-2,4,7,8-tetrachlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 3-OH-2,4,7,8-tetrachlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-OH-2,6,7,8,9-pentachlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 3-OH-2,6,7,8,9-pentachlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-OH-2,6,7,8-tetrachlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 3-OH-2,6,7,8- etrachlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-OH-4,5,3',2',3',4'- tetrachlorated biphenyl
adenosine 3',5'-bisphosphate + 3-OH-4,5,3',4'-tetrachlorated biphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4,4'-(OH)2-3,5,3',5'-tetrachlorated biphenyl
adenosine 3',5'-bisphosphate + 4,4'-(OH)2-3,5,3',5'-tetrachlorated biphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hydroxy-2,3,5,3',4'-pentachlorobiphenyl
adenosine 3',5'-bisphosphate + 4-hydroxy-2,3,5,3',4'-pentachlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hydroxy-3,2',3',4',5'-pentachlorobiphenyl
adenosine 3',5'-bisphosphate + 4-hydroxy-3,2',3',4',5'-pentachlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-OH-1,3,6,7-tetrachlorinated dibenzofuran
adenosine 3',5'-bisphosphate + 4-OH-1,3,6,7-tetrachlorinated dibenzofuranyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-OH-2',4',6'-tribromodiphenyl ether
adenosine 3',5'-bisphosphate + 4-OH-2',4',6'-tribromodiphenyl ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-OH-3',2',4',6'-tetrabromodiphenyl ether
adenosine 3',5'-bisphosphate + 4-OH-3',2',4',6'-tetrabromodiphenyl ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-OH-3',5,2',4',6'-pentabromodiphenyl ether
adenosine 3',5'-bisphosphate + 4-OH-3',5,2',4',6'- pentabromodiphenyl ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-OH-3,3',4'-trichlorated biphenyl
adenosine 3',5'-bisphosphate + 4-OH-3,3',4'-trichlorated biphenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + butylated hydroxyanisole
adenosine 3',5'-bisphosphate + butylated hydroxyanisole sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + catechin hydrate
adenosine 3',5'-bisphosphate + catechin hydrate sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + daidzein
adenosine 3',5'-bisphosphate + daidzein sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + dopamine sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + epicatechin
adenosine 3',5'-bisphosphate + epicatechin sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + epigallocatechin gallate
adenosine 3',5'-bisphosphate + epigallocatechin gallate sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrogen
adenosine 3',5'-bisphosphate + estrogen sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + genistein
adenosine 3',5'-bisphosphate + genistein 7-sulfate + genistein 4'-sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + kaempferol
adenosine 3',5'-bisphosphate + kaempferol sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + myricetin
adenosine 3',5'-bisphosphate + myricetin sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + n-propyl gallate
adenosine 3',5'-bisphosphate + n-propyl gallate sulfate
show the reaction diagram
-
-
r
3'-phosphoadenylylsulfate + p-nitrophenol
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + quercetin
adenosine 3',5'-bisphosphate + quercetin sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + resveratrol
adenosine 3',5'-bisphosphate + resveratrol sulfate
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + 17beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 2-methyl-17beta-estradiol
adenosine 3',5'-bisphosphate + 2-methyl-17beta-estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3alpha-hydroxytibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis
-
-
?
3'-phosphoadenylyl sulfate + 3beta-hydroxytibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estrogen
adenosine 3',5'-bisphosphate + estrogen 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estrone
adenosine 3',5'-bisphosphate + estrone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrogen
adenosine 3',5'-bisphosphate + estrogen sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-6beta-hydroxy-15,16-epoxylabda-8,13(16),14-trien-7-one
-
-
(+)-hispanone
-
-
(-)-6,9:15,16-diepoxy-9alpha-hydroxy-8,9-seco-13(16),14-labdadiene-7-one
-
-
16alpha-hydroxyestrone
-
maximum inhibition at 100 nM
17beta-estradiol
2,4,6-tribromophenol
-
-
2,4,6-Trichlorophenol
-
-
2,4,6-triiodophenol
-
-
2,6-Dichloro-4-nitrophenol
2-hydroxy-1,3,7,8-tetrachlorodibenzo-p-dioxin
-
-
2-hydroxy-3,4,2',3',4'-pentachlorobiphenyl
-
-
2-hydroxy-3,4,3',4'-tetrachlorobiphenyl
-
-
2-hydroxy-4,5,3',4'-tetrachlorobiphenyl
-
-
2-hydroxy-7,8-dichlorodibenzo-p-dioxin
-
-
2-hydroxy-7,8-dichlorodibenzofuran
-
-
2-OH-3,2'-dibromobiphenyl
-
-
-
3',4',7-trihydroxyisoflavone
-
-
3'-phosphoadenylylsulfate
-
-
3,3',5,5'-tetrachlorobisphenol A
-
-
3,3'-(hydroxy)2-4,4'-dichlorobiphenyl
-
-
-
3,5-dibromo-4-hydroxy-benzoic acid (6-chloro-4-oxo-4H-chromen-3-ylmethylene)-hydrazide
-
DBHM
3,5-dibromo-4-hydroxybenzoic acid (6,8-dichloro-4-oxo-4H-chromen-3-ylmethylene) hydrazide
-
DBHD
3-hydroxy-2,'4',5',2',3',4',5'-heptachlorobiphenyl
-
-
-
3-hydroxy-2,4,5,3',4'-pentachlorobiphenyl
-
-
-
3-hydroxy-2,4,7,8,9-pentachlorodibenzofuran
-
-
-
3-hydroxy-2,4,7,8-tetrachlorodibenzofuran
-
-
-
3-hydroxy-4,5,2',3',4'-pentachlorobiphenyl
-
-
-
3-hydroxy-4,5,3',4',5'-pentachlorobiphenyl
-
-
-
3-hydroxy-4,5,3',4'-tetrachlorobiphenyl
-
-
-
3-OH-2,2'-dibromobiphenyl
-
-
-
3-OH-4,4'-dibromobiphenyl
-
-
-
3alpha-tibolone
-
activates SULT1E1 in MCF-7 cells by 50% and 37% at low concentrations of 50 nM and 500 nM, respectively, inhibits at 0.05 mM by 63%
3beta-tibolone
-
activates SULT1E1 in MCF-7 cells by 101% and 74% at low concentrations of 50 nM and 500 nM, respectively, inhibits at 0.05 mM by 54%
4,4'-(hydroxy)2-3,5,'3',5'-tetrachlorobiphenyl
-
-
4,4'-isopropyl idenediphenol-3,3',5,5'-tetrachlorobisphenol
-
-
4,4'-isopropylidenediphenol
-
-
4-ene tibolone
-
inhibits at high concentrations of 0.05 mM by 59%
4-hydroxy-2',3',4',5'-tetrachlorobiphenyl
-
-
4-hydroxy-2',4',6'-trichlorobiphenyl
-
-
4-hydroxy-2,2',3',4',5'-pentachlorobiphenyl
-
-
4-hydroxy-2,2',3',4',6'-pentachlorobiphenyl
-
-
4-hydroxy-2,2',3',5',6'-pentachlorobiphenyl
-
-
4-hydroxy-2,2',4',6'-tetrachlorobiphenyl
-
-
4-hydroxy-2,3,4,5,6-pentabromodiphenylether
-
-
4-hydroxy-2,3,5,2',3',4',5'-heptachlorobiphenyl
-
-
4-hydroxy-2,3,5,2',3',4'-hexachlorobiphenyl
-
-
4-hydroxy-2,3,5,2',4',5'-hexachlorobiphenyl
-
-
4-hydroxy-2,3,5,3',4'-pentachlorobiphenyl
-
-
4-hydroxy-2,3,5,6,2',4',5'-heptachlorobiphenyl
-
-
4-hydroxy-3,2',3',4',5'-pentachlorobiphenyl
-
-
4-hydroxy-3,2',3',4',6'-pentachlorobiphenyl
-
-
4-hydroxy-3,2',3',5',6'-pentachlorobiphenyl
-
-
4-hydroxy-3,2',4',6'-tetrachlorobiphenyl
-
-
4-hydroxy-3,3',4'-trichlorobiphenyl
-
-
4-hydroxy-3,5,2',3',4',5'-hexachlorobiphenyl
-
-
4-hydroxy-3,5,2',3',4'-pentachlorobiphenyl
-
-
4-hydroxy-3,5,3',4',5'-pentachlorobiphenyl
-
-
4-hydroxy-3,5,3',4'-tetrachlorobiphenyl
-
-
4-hydroxy-3,5,3',5'-tetrachlorobiphenyl
-
-
4-hydroxybenzoate esters
-
i.e.parabens, structure-activity relationship, overview
4-hydroxybenzoic acid
-
-
4-OH-2,2'-dibromobiphenyl
-
-
-
4-OH-3,4'-dibromobiphenyl
-
-
-
4-OH-4'-monobromobiphenyl
-
-
-
5,7-dichlorokynurenic acid
-
-
6-OH-2,2'-dibromobiphenyl
-
-
-
Acetylsalicylate
-
binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex
adenosine 5'-(beta,gamma-imido) triphosphate
-
i.e. AMP-PNP, a non-hydrolysable ATP analogue
buthionine sulfoxamine
-
a GSH depletor, decreases SULT1E1 activity, but fails to affect the enzyme amount in Hep-G2 cells
butylparaben
-
50% inhibition at 0.021 mM, complete inhibition at 1 mM; strong inhibition, which is threefold higher in vivo than in vitro
Chloroform
-
excess chloroform inactivates
daidzein
-
-
daidzein 4,7-bisulfate
-
IC50: 0.01 mM
-
diclofenac
-
-
equol
-
potent mixed type inhibitor
ethylparaben
-
; 50% inhibition at 0.080 mM
formononetin
-
-
gavestinel
-
i.e. 4,6-dichloro-3-[(1E)-3-oxo-3-(phenylamino)-1-propenyl]-1H-indole-2-carboxylic acid sodium salt
genistein
-
potent mixed type inhibitor
GSSG
-
oxidized glutathione, inactivation and kinetics, adenosine 3'-phosphate 5'-phosphosulfate protects
hydroxylated polychlorated biphenyls
-
-
-
Ibuprofen
-
-
ICI 182,720
-
produces a marked decrease (over 50%) of EST activity with respect to untreated control cell cultures at 24 h
indomethacin
-
-
Kynurenic acid
-
-
L689,560
-
i.e. trans-2-carboxy-5,7-dichloro-4-phenylaminocarboxylamino-1,2,3,4-tetrahydroquinoline
L701,324
-
i.e. 7-chloro-4-hydroxy-3(3-phenoxy)phenyl-2(H)-quinolinone
leosibiric acid A
-
most potent inhibition
leosibiric acid B
-
-
meclofenamate
-
binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex
Meclofenamic acid
-
-
medrogestone
-
biphasic effect, inhibits at high concentrations, but stimulates at low concentrations, overview
Mefenamic acid
-
-
methylparaben
-
; 50% inhibition at 0.330 mM
naproxen
-
-
nimesulide
-
binds near enough to the substrate site to prevent catalysis but not affect dissociation of the substrate-enzyme complex
OrgOD14
-
active substance in Livial (r), above 0.05 mM decreases significantly biosynthesis of estrone by 50-60%, no effect in MDA-MB-231 cells
Pentachlorophenol
-
-
piroxicam
-
-
Promegestone
-
activates SULT1E1 by 26% at low concentrations of 500 nM, inhibits at 0.05 mM by 41%, leads to increased and decreased expression levels, respetively; biphasic effect, inhibits at high concentrations, but stimulates at low concentrations, overview
propylparaben
-
; 50% inhibition at 0.030 mM
pyridoxal 5'-phosphate
-
a competitive inhibitor for sulfotransferases
quercetin
resveratrol
salicylate
-
-
salicylic acid
-
-
sulindac
-
-
tamoxifen
-
weak inhibition
tibolone
-
activates SULT1E1 in MCF-7 cells by 63% and 44% at low concentrations of 50 nM and 500 nM, respectively, inhibits at 0.05 mM by 64%
trans-resveratrol
-
0.1 mM resveratrol significantly decreases the SULT1E1-mediated beta-estradiol sulfation
triclosan
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17-hydroxy-19-nor-7-methyl-17-pregn-5(10)-en-20-yn-3-one
3alpha-hydroxy-tibolone
-
biphasic effect, inhibits at high concentrations, but stimulates at low concentrations, in MCF-7 and T4-7D cells, overview
3alpha-tibolone
-
activates SULT1E1 in MCF-7 cells by 50% and 37% at low concentrations of 50 nM and 500 nM, respectively, inhibits at 0.05 mM by 63%
3beta-hydroxy-tibolone
-
biphasic effect, inhibits at high concentrations, but stimulates at low concentrations, in MCF-7 and T4-7D cells, overview
3beta-hydroxytibolone
-
102% stimulation of activity at 0.0005 mM
3beta-tibolone
-
activates SULT1E1 in MCF-7 cells by 101% and 74% at low concentrations of 50 nM and 500 nM, respectively, inhibits at 0.05 mM by 54%
4-ene-tibolone
-
-
celecoxib
-
-
medrogestone
Melatonin
-
the level of EST mRNA steady-state of cells treated with melatonin is three times higher than that in control cells
N-acetylcysteine
-
a GSH inducer, increases SULT1E1 activity, but fails to affect the enzyme amount in Hep-G2 cells
NaCl
-
activity of liver EST transiently expressed in COS-1 cells increases 86% in presence of 150 mM
nomegestrol acetate
progesterone
-
specific induction by 0.01 mM, 7fold increase in sulfation of beta-estradiol, Ishikawa endometrial adenocarcinoma cells
Promegestone
quercetin
-
-
resveratrol
-
-
tibolone
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000005 - 0.0082
17beta-estradiol
0.000005
3'-phosphoadenylyl sulfate
-
pH and temperature not specified in the publication
0.000059 - 0.01318
3'-phosphoadenylylsulfate
0.00018
3alpha-tibolone
-
pH 6.5, 37C, recombinant SULT1E1
0.00048
3beta-tibolone
-
pH 6.5, 37C, recombinant SULT1E1
0.15
4-nitrophenol
-
pH 6.1, 25C
0.000038
adenosine 3',5'-bisphosphate
-
pH 6.3, 37C
0.0053
apigenin
-
pH 6.5, 37C, recombinant SULT1E1; pH 6.5, 37C, SULT1E1
0.00033
beta-estradiol
-
isoform SULT1E1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.0045
chrysin
-
pH 6.5, 37C, recombinant SULT1E1; pH 6.5, 37C, SULT1E1
0.00005 - 0.0607
dehydroepiandrosterone
0.2407 - 79.35
dopamine
0.962
epicatechin
-
pH 6.5, 37C, recombinant SULT1E1; pH 6.5, 37C, SULT1E1
0.000004 - 0.0000105
estradiol
0.0001 - 0.0166
estrone
0.000007
ethinyl estradiol
-
pH 7.4, SULT1E1
0.0766 - 0.9372
p-nitrophenol
0.00032 - 0.002
quercetin
0.00058 - 0.0069
resveratrol
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00267 - 0.0217
17beta-estradiol
0.52
4-nitrophenol
-
pH 6.1, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00011 - 0.694
3-cyano-7-hydroxycoumarin
0.0009 - 0.151
4-nitrophenol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000013 - 0.00008
17beta-estradiol
0.00006
2-hydroxy-1,3,7,8-tetrachlorodibenzo-p-dioxin
-
pH 7.2, 37C
0.000002
2-hydroxy-7,8-dichlorodibenzo-p-dioxin
-
pH 7.2, 37C
0.00027
2-hydroxy-7,8-dichlorodibenzofuran
-
pH 7.2, 37C
0.002
3',4',7-trihydroxyisoflavone
-
-
0.000155
3'-phosphoadenylylsulfate
-
pH 6.3, 37C
0.000035
3,3',5,5'-tetrachlorobisphenol A
-
pH 7.2, 37C
0.00000615
3-hydroxy-2,4,7,8,9-pentachlorodibenzofuran
-
pH 7.2, 37C
-
0.014
4,4'-isopropylidenediphenol
-
pH 7.2, 37C
0.00015
4-hydroxy-2,3,4,5,6-pentabromodiphenylether
-
pH 7.2, 37C
0.002
daidzein
-
-
0.0004
equol
-
-
0.006
formononetin
-
-
0.0005
genistein
-
-
0.00058
quercetin
-
pH 7.4, 37C
0.00036 - 0.0023
resveratrol
additional information
additional information
-
inactivation kinetics with oxidized glutathione
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0313
(+)-6beta-hydroxy-15,16-epoxylabda-8,13(16),14-trien-7-one
Homo sapiens
-
at pH 6.0 and 37C
0.0198
(+)-hispanone
Homo sapiens
-
at pH 6.0 and 37C
0.0405
(-)-6,9:15,16-diepoxy-9alpha-hydroxy-8,9-seco-13(16),14-labdadiene-7-one
Homo sapiens
-
at pH 6.0 and 37C
1.5
Acetylsalicylate
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.012 - 0.062
butylparaben
0.01
daidzein 4,7-bisulfate
Homo sapiens
-
IC50: 0.01 mM
-
0.14
Ibuprofen
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.0079
leosibiric acid A
Homo sapiens
-
at pH 6.0 and 37C
0.0232
leosibiric acid B
Homo sapiens
-
at pH 6.0 and 37C
0.0065
meclofenamate
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.0054
Meclofenamic acid
Homo sapiens
-
at pH 6.0 and 37C
0.19
naproxen
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.23
nimesulide
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.27 - 9.4
piroxicam
0.91
salicylic acid
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
0.038
sulindac
Homo sapiens
-
pH 6.5, 37C, versus beta-estradiol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000000005
-
NHEK cell enzyme, cytosol, substrate beta-estradiol
0.0000000001
-
skin enzyme, cytosol, substrate estrone
0.0000000008
-
skin enzyme, cytosol, substrate dehydroepiandrosterone
0.0000000011
-
skin enzyme, cytosol, substrate beta-estradiol
0.0000000049
-
recombinant SULT1E1
0.0000000185
-
liver enzyme, cytosol, substrate beta-estradiol
0.000158
-
catechin hydrate as substrate
0.000171
-
daidzein as substrate
0.000193
-
genistein as substrate
0.000343
-
epigallocatechin gallate as substrate
0.000351
-
epicatechin as substrate
0.000393
-
butylated hydroxyanisole as substrate
0.000452
-
kaempferol as substrate
0.000505
-
17alpha-ethynylestradiol as substrate
0.000526
-
estrone as substrate
0.000594
-
myricetin as substrate
0.000597
-
n-propyl gallate as substrate
0.00061
-
quercetin as substrate
0.012
-
purified recombinant SULT1E1 with substrate estrone
0.0455
-
purified recombinant SULT1E1 with substrate 17beta-estradiol
additional information
-
activity in healthy and hyperplasic endometrium with or without polycystic ovary syndrome, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
assay at
7
-
assay at
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
placental enzyme, isoelectrofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Ishikawa endometrial adenocarcinoma
Manually annotated by BRENDA team
-
fetal, isoform SULT1E1
Manually annotated by BRENDA team
-
female aortic smooth muscle cell line CRL-1999 cell
Manually annotated by BRENDA team
-
benign, and primary and secondary malignant bone tumors, from patients undergoing routine surgery for skeletal tumors
Manually annotated by BRENDA team
-
stromal cells
Manually annotated by BRENDA team
-
high activity in choroid plexus, isoform SULT1E1
Manually annotated by BRENDA team
-
specific expression of SULT1E1
Manually annotated by BRENDA team
-
recombinant SULT1E1 expressed in insect Sf9 cells
Manually annotated by BRENDA team
-
from from 14 patients operated for symptomatic cholelithiais
Manually annotated by BRENDA team
-
an osteosarcoma cell line, increased expression compared to healthy cells
Manually annotated by BRENDA team
-
epidermal
Manually annotated by BRENDA team
-
fetal, isoform SULT1E1
Manually annotated by BRENDA team
-
fetal and adult, isoform SULT1E1
Manually annotated by BRENDA team
-
estrogen sulfotransferase is significantly more active in the normal breast cell than in the cancer cell
Manually annotated by BRENDA team
-
an osteosarcoma cell line, high SULT1E1 expression level
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
intratumoral stromal cell
Manually annotated by BRENDA team
-
hormone-dependent breast cancer cell line
Manually annotated by BRENDA team
-
a Ewings sarcoma cell line, expression level of SULT1E1 is slightly reduced comapred to heathy cells
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34200
-
calculated from cDNA
35120
-
calculated from amino acid sequence
35123
-
2 * 35123, cDNA calculation
36000
-
SDS-PAGE
62000
-
gel filtration
68000
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
detailed structural analysis and comparison of SULT family enzymes, comparison of substrate binding structures, overview, chemical library preparation and ligand binding screens
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
half-life 2 h, glycerol prevents detectable deterioration of activity over 3 h
30 - 45
-
thermal inactivation at 45C
42
-
the wild type enzyme is inactivated at 42C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage
-
-70C, stable for several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
-
Ni-NTA resin column chromatography, and gel filtration
-
recombinant enzyme
-
recombinant SULT1E1
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EST cDNA cloned and expressed in COS-1 cells
-
EST cDNA subcloned into pKK233-2 and expressed in Escherichia coli XL1-Blue
-
EST cloned and expressed in Escherichia coli K1
-
EST expression analysis in healthy and endometriosis uterine endometrium showing no significant differences
-
EST expression analysis in presence and absence of melatonin
-
EST expression and regulation in invasive breast cancer and ductal carcinoma in situ, overview; expression analysis of the enzyme in healthy and preinvasive and invasive tumor breast tissue
-
expressed in Escherichia coli BL21(DE3) cells
-
expression analysis of EST by real-time RT-PCR in prostate cancer cell lines, overview
-
expression analysis of SULT1E1 in gallbladder tissue
-
expression of GFP-tagged SULT1E1 in HEK-293 cell cytoplasm, subcellular localization study, RT-PCR expression analysis, overview; expression of GFP-tagged SULT1E1 in HEK-293 cells, localization in the cytoplasm
-
expression of SULT1E1 in prostate cancer CAHPV-10 cells leading to changes in the transcriptional expression of selected signal transduction-related genes, real-time RT-PCR expression analysis, overview
-
functional overexpression of SULT1E1 in Escherichia coli strain XL-1 blue cytosol
-
hEST cloned and expressed in Salmonella typhimurium
-
human placental cDNA library constructed in lambda gt11, expression vector encoding estrogen sulfotransferase transfected in human adrenal adenocarcinoma SW-13 cells
-
stable expression of SULT1E1 in MCF-7 cells, expression of His-tagged SULT1E1 using a baculoviral-Spodoptera frugiperda Sf9 cell system
-
SULT1E1 cloned and expressed in Escherichia coli BL21 (DE3)
-
SULT1E1 expression analysis
-
SULT1E1 expression in a prokaryotic expression system
-
SULT1E1, DNA and mino acid sequence determination and analysis, genotyping
-
SULT1E1, quantitative expression analysis in benign and in primary and secondary malignant bone tumors
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is significantly higher in the endometrial carcinoma than in normal endometrial tissues
-
inhibition of cystic fibrosis transmembrane receptor expression in MMNK-1 cells results in the induction SULT1E1 message and activity in Hep-G2 cells
-
insulin-like growth factor-1 increases SULT1E1 mRNA and SULT1E1 protein levels
-
reduced SULT1E1 expression is observed in the eutopic endometrium relative to the superficial lesions as well as during the follicular phase of the menstrual cycle
-
SULT expression in breast cancer cells can be stimulated by promegestone, SULT1E1 expression can be controlled by progesterone during the menstrual cycle and during early pregnancy
-
the enzyme expression is downregulated in ovarian cancer; there are significantly higher mRNA levels in ectopic endometrium of deep infiltrating endometriosis, when compared to the normal endometrium. The enzyme expression is upregulated in endometrial cancer
-
the enzyme expression is low in preadipocytes but increases upon differentiation
-
the increase in SULT1E1 activity results in the alteration of estradiol-regulated protein expression in cystic fibrosis liver, SULT1E1 expression in HepG2 cells is inducible by sterol mediated liver-X-receptor activation although not by progestins that induce SULT1E1 in the endometrium
-
WY14643 decreases SULT1E1 mRNA and SULT1E1 protein levels
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D65N/K85E/E86K/F138Y
-
the mutant exhibits increased thermostability (heat inactivation at 43.4C) compared to the wild type enzyme (42C)
E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K
-
the mutant exhibits increased thermostability (heat inactivation at 43.1C) compared to the wild type enzyme (42C)
E12G/N29D/F138Y/G182S/L251V
-
the mutant exhibits increased thermostability (heat inactivation at 51.8C) compared to the wild type enzyme (42C)
E12G/N29D/V73A/N87D/F138Y/G182S/L195M/E211G/E285G
-
the mutant exhibits increased thermostability (heat inactivation at 52.1C) compared to the wild type enzyme (42C)
F138Y/G182S/H224K/E285G
-
the mutant exhibits increased thermostability (heat inactivation at 54.6C) compared to the wild type enzyme (42C)
F138Y/G182S/L195M/E211G
-
the mutant exhibits increased thermostability (heat inactivation at 55.8C) compared to the wild type enzyme (42C)
H107A
-
site-directed mutagenesis, humSULT1E1, presence of a histidine residue at position 107 is essential for the production of a functional protein, substitution of this amino acid to alanine results in complete loss of activity towards all substrates tested
H224Q
-
naturally ocuring mutation in SULT1E1, genotyping in Jewish women of the Ashkenazi ethnic origin
K85A
-
site-directed mutagenesis, humSULT1E1
K85E/A135T/F138Y/G182S/L251V
-
the mutant exhibits increased thermostability (heat inactivation at 46.3C) compared to the wild type enzyme (42C)
N29D/L195M/H224K/L251V
-
the mutant exhibits increased thermostability (heat inactivation at 48.3C) compared to the wild type enzyme (42C)
V145E
-
site-directed mutagenesis, humSULT1E1
V73A/N87D/G182S/H224K/L251V/E285G
-
the mutant exhibits increased thermostability (heat inactivation at 52.8C) compared to the wild type enzyme (42C)
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
pharmacology
-
reactivation of endogenous SULT1E1 gene expression may represent a novel therapeutic strategy to inhibit estrogen-dependent growth of breast cancer cells