EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.8.2.4 | 0.00011 |
- |
3-cyano-7-hydroxycoumarin |
mutant enzyme K85E/A135T/F138Y/G182S/L251V, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.0009 |
- |
4-nitrophenol |
mutant enzyme K85E/A135T/F138Y/G182S/L251V, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.01 |
- |
4-nitrophenol |
mutant enzyme E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.059 |
- |
4-nitrophenol |
mutant enzyme D65N/K85E/E86K/F138Y, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.065 |
- |
3-cyano-7-hydroxycoumarin |
mutant enzyme E12G/N29D/D65N/C83R/N87D/M98V/F138Y/G182S/L195M/H224K, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.151 |
- |
4-nitrophenol |
wild type enzyme, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.179 |
- |
3-cyano-7-hydroxycoumarin |
mutant enzyme D65N/K85E/E86K/F138Y, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |
2.8.2.4 | 0.694 |
- |
3-cyano-7-hydroxycoumarin |
wild type enzyme, 7 mM MgCl2, 1.5 mM dithiothreitol, and 20 mM HEPES (pH 7.5), temperature not specified in the publication |
725716 |