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Literature summary for 2.8.2.4 extracted from
- Sulfuryl transfer: the catalytic mechanism of human estrogen sulfotransferase (1998), J. Biol. Chem., 273, 10888-10892.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| EST cloned and expressed in Escherichia coli K1 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17beta-estradiol | partial substrate inhibitor | Homo sapiens |  |
| 3'-phosphoadenylylsulfate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000005 | - |
17beta-estradiol | pH 6.3, 37°C | Homo sapiens | |
| 0.000038 | - |
adenosine 3',5'-bisphosphate | pH 6.3, 37°C | Homo sapiens | |
| 0.000059 | - |
3'-phosphoadenylylsulfate | pH 6.3, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
| 35123 | - |
2 * 35123, cDNA calculation | Homo sapiens |
| 62000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for several days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 17beta-estradiol | - |
Homo sapiens | adenosine 3',5'-bisphosphate + estradiol 3-sulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Homo sapiens |
| dimer | 2 * 35123, cDNA calculation | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EST | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
half-life 2 h, glycerol prevents detectable deterioration of activity over 3 h | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00267 | - |
17beta-estradiol | pH 6.3, 37°C, reverse reaction | Homo sapiens | |
| 0.0217 | - |
17beta-estradiol | pH 6.3, 37°C forward reaction | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000013 | - |
17beta-estradiol | pH 6.3, 37°C | Homo sapiens | |
| 0.00008 | - |
17beta-estradiol | pH 6.3, 37°C | Homo sapiens | |
| 0.000155 | - |
3'-phosphoadenylylsulfate | pH 6.3, 37°C | Homo sapiens | |
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