EC Number |
Storage Stability |
---|
2.8.2.4 | -20°C, 25 mM monothiolglycerol, 20% loss of activity in 1 week |
2.8.2.4 | -20°C, freshly prepared adrenal cytosol loses no estrogen sulfotransferase activity during storage for periods of up to 5 months |
2.8.2.4 | -20°C, partially purified enzyme exhibits large losses of activity within 1 week |
2.8.2.4 | -20°C, partially purified enzyme may be stored for 2-3 weeks without loss of activity |
2.8.2.4 | -70°C, liver cytosol, 50 mM Tris-HCl, pH 7.8, 0.25 M sucrose, 0.5 mM EDTA, 0.1 mM DTT, and 0.02 mM BHT, no loss of activity during storage |
2.8.2.4 | -70°C, stable for several days |
2.8.2.4 | 0°C, fresh cytosol retains 85% of activity after 20 days, 50% after 50 days |
2.8.2.4 | 0°C, partially purified A form of enzyme loses only a small percentage of its activity on standing in ice for a period of 2 weeks, B form loses 50% of its initial activity in this time |