Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.52: beta-N-acetylhexosaminidase

This is an abbreviated version!
For detailed information about beta-N-acetylhexosaminidase, go to the full flat file.

Word Map on EC 3.2.1.52

Reaction

(N-acetyl-D-glucosamine)4
+ 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase, 3-beta-N-acetylglucosaminidase, 65 kDa epididymal boar protein, Am2301, Am2446, At1g65600/F5I14_13, beta-acetylaminodeoxyhexosidase, beta-acetylhexosaminidase, beta-acetylhexosaminidinase, beta-D-hexosaminidase, beta-D-N-acetylhexosaminidase, Beta-GlcNAcase, beta-glucosidase, beta-HEX, beta-HexNAcase, beta-hexosaminidase, beta-hexosaminidase A, beta-hexosaminidase B, beta-hexosaminidase I, beta-hexosaminidase II, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-hexosaminidase, beta-N-acetyl-hexosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, beta-N-acetylhexosaminidase A, beta-N-acetylhexosaminidase B, beta-Nacetylhexosaminidase S, beta-NAGase, beta-NAHA, Beta-NAHASE, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, BmCHI-h, BmGlcNAcase 1, BmGlcNAcase 2, BmNPV CHIA, CbsA, Chi-I, Chi-II, chitobiase, chitobiase 2, CpGV V-CHIA, dispersin B, DmHEXA, DmHEXB, DspB, EC 3.2.1.29, EC 3.2.1.30, endo-beta-N-acetylglucosaminidase, EXC1Y, EXC2Y, exo-acting chitinase, exo-beta-D-N-acetylglucosaminidase, exo-beta-hexosaminidase, exo-type beta-N-acetylhexosaminidase, exochitinase, F3F20.4 protein, FDL, GH84C, GlcNAcase, Hex, Hex A, Hex B, Hex-1, Hex1, Hex2, Hex20, Hex4, HexB, HexD, HEXDC, hEXO1, HEXO2, HEXO3, hexosaminidase, hexosaminidase A, hexosaminidase B, hexosaminidase D, hHexA, lacto-N-biosidase, lnba, lysosomal beta-hexosaminidase, More, N-acetyl-beta-D-hexosaminidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-hexosaminidase, N-acetylglucosaminidase, N-acetylhexosaminidase, NAG-68, NAG-B, NAG1, NAG2, Nag3, NagA, NAGase, NagJ, NagZ, NAHA, NAHase, neutral hexosaminidase C, O-GlcNAcase, Pcb-NAHA1, Sfhex, SSO3039, StrH, zymocin alpha-subunit

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.52 beta-N-acetylhexosaminidase

Purification

Purification on EC 3.2.1.52 - beta-N-acetylhexosaminidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1094fold to near homogeneity
-
113fold
-
13.2fold to homogeneity
-
135fold to homogeneity
-
1396fold to homogeneity
-
1468fold to homogeneity with an activity yield of 20% by four column chromatography steps (anion exchange chromatography and gel filtration)
1500fold
-
1560fold, fairly homogenous
-
160fold to homogeneity
-
195fold to homogeneity
-
2 isozymes, 153fold to homogeneity
-
200fold to homogeneity
-
226fold to homogeneity
-
241fold, partial
-
2500fold, 5 major isoenzymes
-
256fold to homogeneity
-
30fold
310fold
-
330fold
Helicella ericetorum
-
3350fold to homogeneity
-
387fold to homogeneity
-
4 isozymes, beta-NAHA-II was purified 3200fold to apparent homogeneity, beta-NAHA-IA 770fold, beta-NAHA-IB 1750fold, beta-NAHA-IC 500fold
-
40fold to homogeneity
46.7 fold to homogeneity
Sclerotinia fructigena
-
64fold to homogeneity
-
7000fold to homogeneity
-
A form, still heterogenous: 63fold, B form, close to homogeneity: 92fold
-
A form: 310fold, B form: 260fold, to homogeneity
-
affinity chromatography
-
ammonium sulfate fractionation
beta-hexosaminidase II: 202fold , beta-hexosaminidase III: 426fold, still very heterogenous
-
by ion exchange and gel filtration, to homogeneity, 3.9fold
-
colonic mucosa: form A 4800fold, form B 5110fold, form I 4100fold, colonic tumors: form A 5090fold, form B 5620fold
-
Ex 1: 3000fold, Ex 2: 3200fold
-
form A: 1300-1500fold to homogeneity
-
form I: 185fold, form II: 670fold, form III: 20880fold, form IV: 4740fold
-
form I: 2262fold, form II: 2102fold, form III: 20380fold, form IV: 5061fold
-
forms PI and PII, each 200fold
-
gel filtration
gene lnbA, recombinant His6-tagged enzyme about 2fold from Escherichia coli strain BL21(DE3) by ultrafiltration, nickel affinity chromatography, dialysis and ultrafiltration
Hex A: 161fold, Hex B: 189fold
-
hexosaminidase A, B and I2 purified 2699, 10320 and 13077fold
-
His Trap column chromatography
His-bind resin column chromatography
-
His-Trap column chromatography
HisTrap nickel column chromatography and S200 gel filtration
-
isoenzyme II
-
isoform I and II
-
liver enzyme type S 13500fold, brain enzyme type S 5240fold, liver enzymes types A and B
-
low molecular weight form EII, 3500fold to homogeneity
-
lysosomal enzyme 550fold, microsomal enzyme 756fold, egg white-enzyme 2206fold
-
MagneHis protein purification system
N-acetylglucosaminidase and -galactosaminidase
-
N-acetylhexosaminidase: 63fold
-
N-acetylhexosaminidase: 63fold, N-acetylglucosaminidase and -galactosaminidase
-
native enzyme
-
native enzyme 380.5fold from liver by ammonium sulfate fractionation, by gel filtration, and by chitin affinity, anion exchange, and hydroxy apatite chromatography
-
native Hex1 from SE cells partly by ammonium sulfate fractionation, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
A7XUU4, Q309C3
native Hex2 from SE cells partly by ammonium sulfate fractionation, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
A7XUU4, Q309C3
neutral N-acetylgalactosaminidase, 2000-2500fold to homogeneity
-
neutral N-acetylglucosaminidase, 300-330fold, 85-90% pure
-
Ni-NTA column chromatography
-
Ni2+-NTA agarose column chromatography
Ni2+-NTA Sefinose resin column chromatography
partial
partial purification, 30fold, more extensive purification requires bovine serum albumin for retention of activity
-
partial, 132.9-176.6fold
-
partial, 5-10fold
-
phenyl-Sepharose column chromatography, SP-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
Q-Sepharose column chromatography
recombinant enzyme
-
recombinant enzyme. HexA is purified with immobilized metal affinity column for the His tag attached to the beta-subunit
recombinant His-tagged enzyme fragments
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration
recombinant His-tagged wild-type isozymes Hex1 and Hex2, and of Hex1 from Escherichia coli strain BL21(DE3) by affinity chromatography, followed by ion exchange and hydrophobic chromatography
-
SDS-PAGE and affinity chromatography
SpHex-MBP fusion protein
-
stage-specific enzyme: 2200fold, vegetative enzyme: 1400fold
-
Super Q Toyopearl column chromatography
-
to homogeneity
to homogeneity, 1200fold
-
to homogeneity, 1564fold
-
to homogeneity, normal enzyme 64863fold, I-cell enzyme 20140fold
-
types I-IV 150fold, type I to homogeneity
-