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Literature summary for 3.2.1.52 extracted from

  • Dewji, N.N.; De-Keyser, D.R.; Stirling, J.L.
    Purification and characterization of beta-N-acetylhexosaminidase I2 from human liver (1986), Biochem. J., 234, 157-162.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase B Homo sapiens
0.8
-
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase A Homo sapiens
1
-
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside hexosaminidase I2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
hexosaminidase B Homo sapiens
56000
-
hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE Homo sapiens
100000
-
hexosaminidase A Homo sapiens
100000 110000 hexosaminidase I2, gradient gel electrophoresis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Homo sapiens
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hexosaminidase A, B and I2 purified 2699, 10320 and 13077fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
130
-
hexosaminidase I2, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens
150
-
hexosaminidase A, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens
320
-
hexosaminidase B, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, hexosaminidase A, B and I2 separated from each other Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Homo sapiens 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Homo sapiens N-acetyl-D-hexosamines
-
?

Subunits

Subunits Comment Organism
dimer hexosaminidase I2, major components: 1* 56000 + 1 + 29000, minor components: 65000 - 57000, SDS-PAGE Homo sapiens
dimer hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE Homo sapiens