KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | hexosaminidase B | Homo sapiens | |
0.8 | - |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | hexosaminidase A | Homo sapiens | |
1 | - |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | hexosaminidase I2 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
hexosaminidase B | Homo sapiens |
56000 | - |
hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE | Homo sapiens |
100000 | - |
hexosaminidase A | Homo sapiens |
100000 | 110000 | hexosaminidase I2, gradient gel electrophoresis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Homo sapiens | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hexosaminidase A, B and I2 purified 2699, 10320 and 13077fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
hexosaminidase I2, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | Homo sapiens |
150 | - |
hexosaminidase A, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | Homo sapiens |
320 | - |
hexosaminidase B, substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside | Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, hexosaminidase A, B and I2 separated from each other | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Homo sapiens | N-acetyl-D-hexosamines | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | hexosaminidase I2, major components: 1* 56000 + 1 + 29000, minor components: 65000 - 57000, SDS-PAGE | Homo sapiens |
dimer | hexosaminidase A, major components: 1 * 56000 + 1 * 29000, minor components: 26000 - 22000, SDS-PAGE | Homo sapiens |