Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chondroitin 4-sulfate | 0.025 mg/ml, 1.19fold activation | Palythoa caribaeorum | |
chondroitin 6-sulfate | 0.025 mg/ml, 1.35fold activation | Palythoa caribaeorum | |
Phenylmethylsulfonylfluoride | 1 mM, 1.46fold activation | Palythoa caribaeorum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | 3 mM, 26% inhibition | Palythoa caribaeorum | |
D-mannose | 3 mM, 19% inhibition | Palythoa caribaeorum | |
fucoidan | 0.025 mg/ml, 19% inhibition | Palythoa caribaeorum | |
HgCl2 | 1 mM, complete inhibition | Palythoa caribaeorum | |
iodoacetamide | 1 mM, 16% inhibition | Palythoa caribaeorum | |
lactose | 3 mM, 20% inhibition | Palythoa caribaeorum | |
maltose | 3 mM, 33% inhibition | Palythoa caribaeorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
Palythoa caribaeorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 1 mM, 1.4fold activation | Palythoa caribaeorum | |
MgCl2 | 1 mM, 1.17fold activation | Palythoa caribaeorum | |
Na2SeO4 | 1 mM, 1.45fold activation | Palythoa caribaeorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Palythoa caribaeorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Palythoa caribaeorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Palythoa caribaeorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
57.9 U/mg. One unit of enzyme activity was defined as the amount of enzyme which released 0.01 absorbance at 405 nm | Palythoa caribaeorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose + H2O | - |
Palythoa caribaeorum | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside + H2O | preferentially hydrolyzed | Palythoa caribaeorum | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
additional information | no activity with chitin azure | Palythoa caribaeorum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Palythoa caribaeorum |
Synonyms | Comment | Organism |
---|---|---|
Pcb-NAHA1 | - |
Palythoa caribaeorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | - |
Palythoa caribaeorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 70 | 37°C: about 60% of maximal activity, 70°C: about 25% of maximal activity | Palythoa caribaeorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside | - |
Palythoa caribaeorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Palythoa caribaeorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | pH 4.0: about 35% of maximal activity, pH 5.5: about 35% of maximal activity, pH 8.5: about 50% of maximal activity | Palythoa caribaeorum |