Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | with 30 mM 1.1% of activity remains | Brassica oleracea | |
N-bromosuccinimide | with 0.025 mM 4.4% of activity remains | Brassica oleracea | |
N-ethyl-5-phenyl-isoxazolium-3'-sulfonate | with 30 mM 23.3% of activity remains | Brassica oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE | Brassica oleracea |
57000 | - |
1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE | Brassica oleracea |
64000 | - |
1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE | Brassica oleracea |
150000 | - |
gel filtration | Brassica oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Brassica oleracea | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
256fold to homogeneity | Brassica oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.737 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O | - |
Brassica oleracea | 4 N-acetyl-D-glucosamine | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Brassica oleracea | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Brassica oleracea | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Brassica oleracea | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Brassica oleracea | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
tri-N-acetylchitotriose + H2O | - |
Brassica oleracea | N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 64000 + 1 * 57000 + 1 * 51000, SDS-PAGE | Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Brassica oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable below for 15 min at pH 7.0 | Brassica oleracea |
35 | 60 | for 15 min at pH 7.0, gradual inactivation | Brassica oleracea |
65 | - |
for 15 min at pH 7.0, rapid inactivation | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Brassica oleracea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | stable for 4 hr at room temperature | Brassica oleracea |
10 | - |
rapidly inactivated above, at room temperature | Brassica oleracea |