Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-acetyl-glucosamine | 0.05% (w/v) | Cellulomonas fimi | |
N-acetylglucosamine | 0.05% (w/v) | Cellulomonas fimi |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cellulomonas fimi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
4-nitrophenyl beta-N-acetyl-D-glucosaminide | 50 mM sodium phosphate buffer (pH 7.08) at 25 °C | Cellulomonas fimi | |
0.053 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 4-nitrophenyl-beta-N-acetyl-D-glucosaminide | Cellulomonas fimi | |
0.066 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 4-nitrophenyl-beta-N-acetyl-D-galactosaminide | Cellulomonas fimi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54190 | - |
electrospray mass spectroscopy | Cellulomonas fimi |
60970 | - |
electrospray mass spectroscopy | Cellulomonas fimi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas fimi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-bind resin column chromatography | Cellulomonas fimi |
Storage Stability | Organism |
---|---|
-20°C, glycerol (20% final concentration) and phosphate buffer at pH 7.3, several months, loss of catalytic activity in diluted buffers within a day at 4 °C | Cellulomonas fimi |
4°C, elution buffer (20 mM sodium phosphate, 80 mM imidazole pH 7.5 and 300 mM NaCl), several months | Cellulomonas fimi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(25R)-3beta-hydroxycholest-5-en-27-oate + H2O | - |
Cellulomonas fimi | ? | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Cellulomonas fimi | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O | - |
Cellulomonas fimi | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Cellulomonas fimi | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-beta-N-acetylglucosaminidase | - |
Cellulomonas fimi |
beta-glucosidase | - |
Cellulomonas fimi |
beta-N-acetylglucosaminidase | - |
Cellulomonas fimi |
Beta-N-acetylhexosaminidase | - |
Cellulomonas fimi |
Hex20 | - |
Cellulomonas fimi |
Nag3 | - |
Cellulomonas fimi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
4-nitrophenyl beta-N-acetyl-D-glucosaminide | 50 mM sodium phosphate buffer (pH 7.08) at 25 °C | Cellulomonas fimi | |
129 | - |
4-nitrophenyl beta-N-acetyl-D-galactosaminide | 50 mM sodium phosphate buffer (pH 7.08) at 25 °C | Cellulomonas fimi | |
482 | - |
4-nitrophenyl-beta-N-acetyl-D-glucosaminide | 50 mM sodium phosphate buffer (pH 7.08) at 25 °C | Cellulomonas fimi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Cellulomonas fimi |
8 | - |
- |
Cellulomonas fimi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | inactivation above pH 9.5 | Cellulomonas fimi |
6.8 | 8.4 | - |
Cellulomonas fimi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | inactivation above pH 9.5 | Cellulomonas fimi |
6.8 | 8.4 | - |
Cellulomonas fimi |