Literature summary for 3.2.1.52 extracted from

Nieder, V.; Kutzer, M.; Kren, V.; Gallego, R.G.; Kamerling, J.P.; Elling, L.
Screening and characterization of beta-N-acetylhexosaminidases for the synthesis of nucleotide-activated disaccharides (2004), Enzyme Microb. Technol., 34, 407-414.

No PubMed abstract available

Search for more literature for 3.2.1.52

Inhibitors

Inhibitors	Comment	Organism	Structure
N-acetyl-D-galactosamine	-	Trichoderma harzianum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.21	-	4-nitrophenyl beta-N-acetyl-D-galactosaminide	-	Trichoderma harzianum
3.44	-	4-nitrophenyl beta-N-acetyl-D-galactosaminide	in presence of 200 mM heptakis-(2,6-di-O-methyl)-beta-cyclodextrin	Trichoderma harzianum

Organism

Organism	UniProt	Comment	Textmining
Aspergillus flavipes	-	-	-
Aspergillus flavus	-	-	-
Aspergillus oryzae	-	-	-
Aspergillus parasiticus	-	-	-
Aspergillus tamarii	-	-	-
Penicillium oxalicum	-	-	-
Trichoderma harzianum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate fractionation	Aspergillus flavus
ammonium sulfate fractionation	Aspergillus oryzae
ammonium sulfate fractionation	Aspergillus parasiticus
ammonium sulfate fractionation	Trichoderma harzianum
ammonium sulfate fractionation	Penicillium oxalicum
ammonium sulfate fractionation	Aspergillus flavipes
ammonium sulfate fractionation	Aspergillus tamarii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
21	-	-	Aspergillus parasiticus
30	-	-	Penicillium oxalicum
30	-	-	Aspergillus tamarii
34	-	-	Aspergillus flavus
44	-	-	Trichoderma harzianum
49	-	-	Aspergillus oryzae
55	-	-	Aspergillus flavipes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-N-acetyl-D-galactosaminide + UDP-N-acetyl-D-glucosamine + H2O	-	Trichoderma harzianum	UDP-(4-O-N-acetyl-beta-D-galactosaminyl)-N-acetyl-alpha-D-glucosamine + 4-nitrophenol	-	ir
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Trichoderma harzianum	4-nitrophenol + N-acetyl-D-glucosamine	-	?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Aspergillus flavus	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Aspergillus oryzae	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Aspergillus parasiticus	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Aspergillus flavipes	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O	-	Aspergillus tamarii	4-nitrophenol + N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O	-	Trichoderma harzianum	4-nitrophenol + N-acetyl-beta-D-galactosamine	-	?
p-nitrophenyl-beta-N-acetyl-D-glucosaminide + H2O	-	Penicillium oxalicum	N-acetyl-D-glucosamine + p-nitrophenol	-	?

Synonyms

Synonyms	Comment	Organism
Beta-N-acetylhexosaminidase	-	Aspergillus flavus
Beta-N-acetylhexosaminidase	-	Aspergillus oryzae
Beta-N-acetylhexosaminidase	-	Aspergillus parasiticus
Beta-N-acetylhexosaminidase	-	Trichoderma harzianum
Beta-N-acetylhexosaminidase	-	Penicillium oxalicum
Beta-N-acetylhexosaminidase	-	Aspergillus flavipes
Beta-N-acetylhexosaminidase	-	Aspergillus tamarii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Aspergillus flavus
30	-	-	Aspergillus oryzae
30	-	-	Aspergillus parasiticus
30	-	-	Trichoderma harzianum
30	-	-	Penicillium oxalicum
30	-	-	Aspergillus flavipes
30	-	-	Aspergillus tamarii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Aspergillus flavus
6.5	-	-	Aspergillus oryzae
6.5	-	-	Aspergillus parasiticus
6.5	-	-	Trichoderma harzianum
6.5	-	-	Penicillium oxalicum
6.5	-	-	Aspergillus flavipes
6.5	-	-	Aspergillus tamarii

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.21	-	N-acetyl-D-galactosamine	in presence of 200 mM heptakis-(2,6-di-O-methyl)-beta-cyclodextrin	Trichoderma harzianum
1.19	-	N-acetyl-D-galactosamine	-	Trichoderma harzianum

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Release 2023.1 (January 2023)