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3.2.1.52: beta-N-acetylhexosaminidase

This is an abbreviated version!
For detailed information about beta-N-acetylhexosaminidase, go to the full flat file.

Word Map on EC 3.2.1.52

Reaction

(N-acetyl-D-glucosamine)4
+ 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase, 3-beta-N-acetylglucosaminidase, 65 kDa epididymal boar protein, Am2301, Am2446, At1g65600/F5I14_13, beta-acetylaminodeoxyhexosidase, beta-acetylhexosaminidase, beta-acetylhexosaminidinase, beta-D-hexosaminidase, beta-D-N-acetylhexosaminidase, Beta-GlcNAcase, beta-glucosidase, beta-HEX, beta-HexNAcase, beta-hexosaminidase, beta-hexosaminidase A, beta-hexosaminidase B, beta-hexosaminidase I, beta-hexosaminidase II, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-hexosaminidase, beta-N-acetyl-hexosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, beta-N-acetylhexosaminidase A, beta-N-acetylhexosaminidase B, beta-Nacetylhexosaminidase S, beta-NAGase, beta-NAHA, Beta-NAHASE, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, BmCHI-h, BmGlcNAcase 1, BmGlcNAcase 2, BmNPV CHIA, CbsA, Chi-I, Chi-II, chitobiase, chitobiase 2, CpGV V-CHIA, dispersin B, DmHEXA, DmHEXB, DspB, EC 3.2.1.29, EC 3.2.1.30, endo-beta-N-acetylglucosaminidase, EXC1Y, EXC2Y, exo-acting chitinase, exo-beta-D-N-acetylglucosaminidase, exo-beta-hexosaminidase, exo-type beta-N-acetylhexosaminidase, exochitinase, F3F20.4 protein, FDL, GH84C, GlcNAcase, Hex, Hex A, Hex B, Hex-1, Hex1, Hex2, Hex20, Hex4, HexB, HexD, HEXDC, hEXO1, HEXO2, HEXO3, hexosaminidase, hexosaminidase A, hexosaminidase B, hexosaminidase D, hHexA, lacto-N-biosidase, lnba, lysosomal beta-hexosaminidase, More, N-acetyl-beta-D-hexosaminidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-hexosaminidase, N-acetylglucosaminidase, N-acetylhexosaminidase, NAG-68, NAG-B, NAG1, NAG2, Nag3, NagA, NAGase, NagJ, NagZ, NAHA, NAHase, neutral hexosaminidase C, O-GlcNAcase, Pcb-NAHA1, Sfhex, SSO3039, StrH, zymocin alpha-subunit

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.52 beta-N-acetylhexosaminidase

Storage Stability

Storage Stability on EC 3.2.1.52 - beta-N-acetylhexosaminidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, crude culture supernatant
-
-20°C, after 27 days 87.1% of activity remains
-
-20°C, after 6 weeks no significant loss of activity
-
-20°C, buffer comprising 50 mM sodium phosphate, pH 5.8, 50 mm NaCl and 50% glycerol, stable for several months
-
-20°C, crude enzyme, stable
-
-20°C, crude extract
-
-20°C, enzyme preparation concentrated by ultrafiltration
-
-20°C, glycerol (20% final concentration) and phosphate buffer at pH 7.3, several months, loss of catalytic activity in diluted buffers within a day at 4 °C
-
-20°C, in 10 mM sodium acetate buffer pH 4.0, very unstable
-
-20°C, in 5-50 mM sodium citrate buffer pH 4.0, very stable, even after repeated freeze-thawing
-
-20°C, in presence of 20% glycerol stable for at least 2 months, even more stable in 50% glycerol
-
-20°C, low molecular weight form EII, concentrated by ultrafiltration
-
-20°C, lyophilized
-
-20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days
-20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C
-20°C, purified enzyme, stable
-
-20°C, stable for at least 1 year
-
-20°C, stable for several weeks
-
-70°C, hexosaminidase A, B and I2 separated from each other
-
-70°C, in presence of 20% glycerol stable for at least 3 months, even more stable in 50% glycerol
-
-70°C, purified enzyme, stable for at least 6 months
-80°C, crude cell-free extract, 0.25-2.0 mg protein/ml
0-4°C, stable for several days without appreciable loss of activity
-
0°C, half-life of the purified enzyme is 6 days
Sclerotinia fructigena
-
0°C, in 10% glycerol, pH 5.0-6.0, no loss of activity in 4 months
-
2°C, in (NH4)2SO4, stable
-
4°C, after 2 months of storage in phosphate buffer pH 5.0 only form I shows decrease of activity by 50-60%, after 4 months a new form of enzyme with a molecular weight of 62000 Da and a Km of 0.056 mM appears
-
4°C, crude supernatant fraction stable for at least a year
-
4°C, elution buffer (20 mM sodium phosphate, 80 mM imidazole pH 7.5 and 300 mM NaCl), several months
-
4°C, enzyme purified in presence of bovine serum albumin, storage in presence of 0.04% w/v sodium azide, enzyme retains 76% of activity after 5 months
-
4°C, enzyme solution unstable
-
4°C, liver enzyme type S, concentrated by ultrafiltration to 0.18 mg/ml, in 0.04 citrate buffer and in presence of 0.2 mg/ml bovine serum albumin stable for at least 3 months
-
4°C, not stable, rapid decrease of activity after the first 12 days
-
4°C, purified and concentrated enzyme in McIlvaine buffer pH 5.0 conserved with 0.03% (w/v) NaN3, ca 6 months, less than 20% loss of activity
4°C, purified enzyme after sterilization stable for at least 6 months
-
4°C, purified enzyme stable for few weeks
-
4°C, purified enzyme, stable for several months in presence of 30% (NH4)2SO4 in 50 mM phosphate buffer, pH 7.0
-
4°C, stable for 6 weeks
-
4°C, stable for several months
-
4°C, wild type and mutant enzymes, stable for at least 2 weeks
-
enzyme can be stored at -20°C
lyophilization
-
refrigerated temperatures, stable for several days
-