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Literature summary for 3.2.1.52 extracted from

  • Koga, D.; Hoshika, H.; Matsushita, M.; Tanaka, A.; Ide, A.; Kono, M.
    Purification and characterization of beta-N-acetylhexosaminidase from the liver of a prawn, Penaeus japonicus (1996), Biosci. Biotechnol. Biochem., 60, 194-199.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ concentration of 10 mM causes 39% inhibition Penaeus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 (N-acetyl-beta-D-glucosamine)4
-
 Penaeus japonicus
0.045
-
 (N-acetyl-beta-D-glucosamine)5
-
 Penaeus japonicus
0.047
-
 (N-acetyl-beta-D-glucosamine)6
-
 Penaeus japonicus
0.055
-
 (N-acetyl-beta-D-glucosamine)3
-
 Penaeus japonicus
0.117
-
 (N-acetyl-beta-D-glucosamine)2
-
 Penaeus japonicus
0.137
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Penaeus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 SDS-PAGE Penaeus japonicus
110000
-
 gel filtration Penaeus japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Penaeus japonicus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Penaeus japonicus
-
 prawn
-

Purification (Commentary)

Purification (Comment) Organism
699fold Penaeus japonicus

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Penaeus japonicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Penaeus japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
123000
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Penaeus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(N-acetyl-beta-D-glucosamine)2 + H2O
-
 Penaeus japonicus 2 N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)3 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)4 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)5 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)6 + H2O
-
 Penaeus japonicus (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
 ?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Penaeus japonicus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Penaeus japonicus N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Penaeus japonicus ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Penaeus japonicus ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Penaeus japonicus 2 N,N-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Penaeus japonicus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Penaeus japonicus deacetylated chitooligosaccharides + acetate
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Penaeus japonicus N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Penaeus japonicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Penaeus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penaeus japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable below Penaeus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.3
-
 (N-acetyl-beta-D-glucosamine)6
-
 Penaeus japonicus
14.7
-
 (N-acetyl-beta-D-glucosamine)5
-
 Penaeus japonicus
30.1
-
 (N-acetyl-beta-D-glucosamine)4
-
 Penaeus japonicus
96.4
-
 (N-acetyl-beta-D-glucosamine)3
-
 Penaeus japonicus
298
-
 (N-acetyl-beta-D-glucosamine)2
-
 Penaeus japonicus
598
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Penaeus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Penaeus japonicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 stable Penaeus japonicus