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Literature summary for 3.2.1.52 extracted from
- Purification of beta-N-acetyl-D-glucosaminidase of the horseshoe crab by affinity chromatography (1977), J. Chromatogr., 136, 141-146.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-aminophenyl 1-thio-beta-L-fucopyranoside | - |
Limulus polyphemus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | passing through enzyme | Limulus polyphemus | |
| 0.45 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | adherent enzyme | Limulus polyphemus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Limulus polyphemus | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limulus polyphemus | - |
horseshoe crab | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 100fold | Limulus polyphemus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Limulus polyphemus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.067 | 0.117 | substrate: p-nitrophenyl-beta-N-acetyl-D-glucosaminide | Limulus polyphemus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several months | Limulus polyphemus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Limulus polyphemus | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Limulus polyphemus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Limulus polyphemus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
precipitous drop at values below | Limulus polyphemus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | activity is reduced by 23% | Limulus polyphemus |
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