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Literature summary for 3.2.1.52 extracted from

  • Lunetta, J.M.; Johnson, S.M.; Pappagianis, D.
    Molecular cloning, characterization and expression analysis of two beta-N-acetylhexosaminidase homologs of Coccidioides posadasii (2010), Med. Mycol., 48, 744-756.
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
gene CpHEX1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 Coccidioides posadasii
gene CpHEX2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 Coccidioides posadasii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
 x * 68500, about, SDS-PAGE and amino acid sequence calculation Coccidioides posadasii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coccidioides posadasii beta-N-acetylhexosaminidase catalyzes the release of terminal, non-reducing N-acetylglucosamine or N-acetylgalactosamine residues from oligosaccharides ?
-
 ?
additional information Coccidioides posadasii Silveira beta-N-acetylhexosaminidase catalyzes the release of terminal, non-reducing N-acetylglucosamine or N-acetylgalactosamine residues from oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Coccidioides posadasii A7XUU4 CpHEX2; gene CpHEX2
-
Coccidioides posadasii Q309C3 CpHEX1; gene CpHEX1
-
Coccidioides posadasii Silveira A7XUU4 CpHEX2; gene CpHEX2
-
Coccidioides posadasii Silveira Q309C3 CpHEX1; gene CpHEX1
-

Purification (Commentary)

Purification (Comment) Organism
native Hex1 from SE cells partly by ammonium sulfate fractionation, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Coccidioides posadasii
native Hex2 from SE cells partly by ammonium sulfate fractionation, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Coccidioides posadasii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cells in SE phase Coccidioides posadasii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside + 3 H2O
-
 Coccidioides posadasii 4-methylumbelliferone + 3 N-acetylglucosamine
-
 ?
4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside + 3 H2O
-
 Coccidioides posadasii Silveira 4-methylumbelliferone + 3 N-acetylglucosamine
-
 ?
4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside + 2 H2O
-
 Coccidioides posadasii 4-methylumbelliferone + 2 N-acetylgalactosamine
-
 ?
4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside + 2 H2O
-
 Coccidioides posadasii Silveira 4-methylumbelliferone + 2 N-acetylgalactosamine
-
 ?
4-methylumbelliferyl N-acetyl-beta-D-galactosamine + H2O
-
 Coccidioides posadasii 4-methylumbelliferone + N-acetylgalactosamine
-
 ?
4-methylumbelliferyl N-acetyl-beta-D-galactosamine + H2O
-
 Coccidioides posadasii Silveira 4-methylumbelliferone + N-acetylgalactosamine
-
 ?
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + H2O
-
 Coccidioides posadasii 4-methylumbelliferone + N-acetylglucosamine
-
 ?
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + H2O
-
 Coccidioides posadasii Silveira 4-methylumbelliferone + N-acetylglucosamine
-
 ?
additional information beta-N-acetylhexosaminidase catalyzes the release of terminal, non-reducing N-acetylglucosamine or N-acetylgalactosamine residues from oligosaccharides Coccidioides posadasii ?
-
 ?
additional information beta-N-acetylhexosaminidase catalyzes the release of terminal, non-reducing N-acetylglucosamine or N-acetylgalactosamine residues from oligosaccharides Coccidioides posadasii Silveira ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68500, about, SDS-PAGE and amino acid sequence calculation Coccidioides posadasii

Synonyms

Synonyms Comment Organism
chitobiase
-
 Coccidioides posadasii
chitobiase 2
-
 Coccidioides posadasii
Hex1
-
 Coccidioides posadasii
Hex2
-
 Coccidioides posadasii