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3.2.1.52: beta-N-acetylhexosaminidase

This is an abbreviated version!
For detailed information about beta-N-acetylhexosaminidase, go to the full flat file.

Word Map on EC 3.2.1.52

Reaction

(N-acetyl-D-glucosamine)4
+ 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase, 3-beta-N-acetylglucosaminidase, 65 kDa epididymal boar protein, Am2301, Am2446, At1g65600/F5I14_13, beta-acetylaminodeoxyhexosidase, beta-acetylhexosaminidase, beta-acetylhexosaminidinase, beta-D-hexosaminidase, beta-D-N-acetylhexosaminidase, Beta-GlcNAcase, beta-glucosidase, beta-HEX, beta-HexNAcase, beta-hexosaminidase, beta-hexosaminidase A, beta-hexosaminidase B, beta-hexosaminidase I, beta-hexosaminidase II, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-hexosaminidase, beta-N-acetyl-hexosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, beta-N-acetylhexosaminidase A, beta-N-acetylhexosaminidase B, beta-Nacetylhexosaminidase S, beta-NAGase, beta-NAHA, Beta-NAHASE, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, BmCHI-h, BmGlcNAcase 1, BmGlcNAcase 2, BmNPV CHIA, CbsA, Chi-I, Chi-II, chitobiase, chitobiase 2, CpGV V-CHIA, dispersin B, DmHEXA, DmHEXB, DspB, EC 3.2.1.29, EC 3.2.1.30, endo-beta-N-acetylglucosaminidase, EXC1Y, EXC2Y, exo-acting chitinase, exo-beta-D-N-acetylglucosaminidase, exo-beta-hexosaminidase, exo-type beta-N-acetylhexosaminidase, exochitinase, F3F20.4 protein, FDL, GH84C, GlcNAcase, Hex, Hex A, Hex B, Hex-1, Hex1, Hex2, Hex20, Hex4, HexB, HexD, HEXDC, hEXO1, HEXO2, HEXO3, hexosaminidase, hexosaminidase A, hexosaminidase B, hexosaminidase D, hHexA, lacto-N-biosidase, lnba, lysosomal beta-hexosaminidase, More, N-acetyl-beta-D-hexosaminidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-hexosaminidase, N-acetylglucosaminidase, N-acetylhexosaminidase, NAG-68, NAG-B, NAG1, NAG2, Nag3, NagA, NAGase, NagJ, NagZ, NAHA, NAHase, neutral hexosaminidase C, O-GlcNAcase, Pcb-NAHA1, Sfhex, SSO3039, StrH, zymocin alpha-subunit

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.52 beta-N-acetylhexosaminidase

pH Stability

pH Stability on EC 3.2.1.52 - beta-N-acetylhexosaminidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5
-
activity is lost below
393666
10
-
rapidly inactivated above, at room temperature
393687
2
Sclerotinia fructigena
-
complete inactivation after 30 min at 25°C
393724
2 - 4
-
relatively stable, retains 85% of activity after 45 hours at 5°C
393666
2.5 - 10
-
not less than 80% of activity remains, at 5°C for 1 h
393683
3
Sclerotinia fructigena
-
40% inactivation after 30 min at 25°C
393724
3 - 10
3 - 9
3.3
Helicella ericetorum
-
50% of activity are lost
393671
3.5
-
half-maximal activity
393728
3.5 - 5.5
-
stable
393695
3.8 - 4.6
Helicella ericetorum
-
at 37°C stable for 2 hours
393671
4 - 10
-
stable, at 37°C
393692
4 - 11
4 - 4.5
-
37°C, 1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glalactosaminide is stable at
655935
4 - 7.5
4 - 8
-
stable at 30°C for 30 min
393703
4.5
-
at 37°C, stable for 15 min
393725
4.5 - 10
the enzyme keeps over 80% activity after pre-incubation at 4°C for 12 h from pH 4.5-10.0
750077
4.5 - 6.5
-
stable, exochitinase
680953
4.5 - 7
-
at 4°C for 24 hours and at 37°C for 60 min
393682
4.5 - 8.5
the enzyme retains stable at pH 4.5-8.5, with more than 80 % of activity after 1 h-incubation
751148
5 - 5.5
-
37°C, 1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide is stable at
655935
5 - 6
-
stable
393665
5 - 8.5
-
alimentary canal enzyme, stable
393704
5 - 9
-
Ex 2, stable
393704
5.5 - 8
purified enzyme, 37°C, 30 min: over 80% activity remaining, 24 h: 40% activity remaining at pH 5.5, over 80% at pH 6.0-7.0, 30% at pH 8.0, unstable below pH 5.5, residual activity after 30 min incubation at pH 4.0-5.0 is below 20%
731962
5.5 - 8.5
-
stable
393705
6 - 7
-
activity is reduced by 23%
393716
6 - 8
6 - 9.5
-
inactivation above pH 9.5
664914
6.2
-
80% of activity
393706
6.3
Helicella ericetorum
-
50% of activity are lost
393671
6.5
-
stable
393696
6.5 - 8
-
fairly stable
135988
6.8 - 8.4
-
-
664914
7 - 9
-
stable within this range
393660
7.5
no activity, substrate: (N-acetyl-D-glucosamine)2
393685
8 - 8.3
50-60% of activity with (N-acetyl-D-glucosamine)3-6 as substrate, no activity with (N-acetyl-D-glucosamine)2 as substrate
393685
8.5
-
most stable at this pH, stability rapidly decreasing above or below
393722
8.5 - 9
-
inactivation at high pH like 8.5-9.0
26851
9
50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
393685
additional information