Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.52: beta-N-acetylhexosaminidase

This is an abbreviated version!
For detailed information about beta-N-acetylhexosaminidase, go to the full flat file.

Word Map on EC 3.2.1.52

Reaction

(N-acetyl-D-glucosamine)4
+ 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase, 3-beta-N-acetylglucosaminidase, 65 kDa epididymal boar protein, Am2301, Am2446, At1g65600/F5I14_13, beta-acetylaminodeoxyhexosidase, beta-acetylhexosaminidase, beta-acetylhexosaminidinase, beta-D-hexosaminidase, beta-D-N-acetylhexosaminidase, Beta-GlcNAcase, beta-glucosidase, beta-HEX, beta-HexNAcase, beta-hexosaminidase, beta-hexosaminidase A, beta-hexosaminidase B, beta-hexosaminidase I, beta-hexosaminidase II, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-hexosaminidase, beta-N-acetyl-hexosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, beta-N-acetylhexosaminidase A, beta-N-acetylhexosaminidase B, beta-Nacetylhexosaminidase S, beta-NAGase, beta-NAHA, Beta-NAHASE, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, BmCHI-h, BmGlcNAcase 1, BmGlcNAcase 2, BmNPV CHIA, CbsA, Chi-I, Chi-II, chitobiase, chitobiase 2, CpGV V-CHIA, dispersin B, DmHEXA, DmHEXB, DspB, EC 3.2.1.29, EC 3.2.1.30, endo-beta-N-acetylglucosaminidase, EXC1Y, EXC2Y, exo-acting chitinase, exo-beta-D-N-acetylglucosaminidase, exo-beta-hexosaminidase, exo-type beta-N-acetylhexosaminidase, exochitinase, F3F20.4 protein, FDL, GH84C, GlcNAcase, Hex, Hex A, Hex B, Hex-1, Hex1, Hex2, Hex20, Hex4, HexB, HexD, HEXDC, hEXO1, HEXO2, HEXO3, hexosaminidase, hexosaminidase A, hexosaminidase B, hexosaminidase D, hHexA, lacto-N-biosidase, lnba, lysosomal beta-hexosaminidase, More, N-acetyl-beta-D-hexosaminidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-hexosaminidase, N-acetylglucosaminidase, N-acetylhexosaminidase, NAG-68, NAG-B, NAG1, NAG2, Nag3, NagA, NAGase, NagJ, NagZ, NAHA, NAHase, neutral hexosaminidase C, O-GlcNAcase, Pcb-NAHA1, Sfhex, SSO3039, StrH, zymocin alpha-subunit

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.52 beta-N-acetylhexosaminidase

Cloned

Cloned on EC 3.2.1.52 - beta-N-acetylhexosaminidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
characterization of the v-chiA of Cydia pomonella GV (CpGV) by constructing a recombinant Bombyx mori NPV (BmNPV) in which BmNPV v-chiA is replaced by CpGV v-chiA
-
expressed in baculovirus-infected sf9 insect cells
expressed in Escherichia coli
expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells. Plasmid encoding an N-terminally Myc-tagged form of the full-length murine hexosaminidase transfected into murine NIH 3T3 fibroblast cells. Myc-tagged protein transfected into COS cells
expressed in Escherichia coli T7 Express lysY cells
expressed in Pichia pastoris
expressed in Pichia pastoris GS115 cells
-
expressed in Pichia pastoris strain KM71H
expressed in Pichia pastoris strain X33
expressed in Pichia pastoris X-33 cells
expressed in Sf21 cells. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana
expression in Escherichia coli
expression in Escherichia coli, Chi-I
-
expression in Escherichia coli, Chi-II
-
expression in Pichia pastoris
expression of His-tagged enzyme fragments
expression of His-tagged wild-type isozymes Hex1 and Hex2, and of Hex1 mutants in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
full-length cDNA cloned into the pCRs4-TOPOs vector
gene and isozyme BmGlcNAcase 1, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method
gene and isozyme BmGlcNAcase 2, DNA and amino acid sequence determnination and analysis, genetic structure, phylogenetic analysis, and sequence comparisons, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method
gene cbsA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene CpHEX1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21
A7XUU4, Q309C3
gene CpHEX2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21
A7XUU4, Q309C3
gene lnbA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene nagA, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
HEXO1 lacking its 32 N-terminal amino acids is cloned into baculovirus transfer vector pVTBac-His-1 and is expressed in Sf21. HEXO fluorescent protein fusions transiently expressed in Nicotiana benthamiana
into Escherichia coli
ligated with vector pQE-30 and expressed in Escherichia coli BL21(DE3)
-
recombinant His-tagged HexA is produced in the methylotrophic yeast Ogataea minuta (alpha-1,6-mannosyltransferase-deficient). Genes encoding the alpha- and beta-subunits of HexA are integrated into the yeast cell, and the heterodimer is expressed together with its isozymes HexS (alphaalpha) and HexB (betabeta). A total of 57 mg of beta-hexosaminidase isozymes, of which 13 mg was HexA (alphabeta), is produced per liter of medium. The C terminus of the beta-subunit appears to be truncated in Ogataea minuta recombinant HexA