Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.52: beta-N-acetylhexosaminidase

This is an abbreviated version!
For detailed information about beta-N-acetylhexosaminidase, go to the full flat file.

Word Map on EC 3.2.1.52

Reaction

(N-acetyl-D-glucosamine)4
+ 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose

Synonyms

2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase, 3-beta-N-acetylglucosaminidase, 65 kDa epididymal boar protein, Am2301, Am2446, At1g65600/F5I14_13, beta-acetylaminodeoxyhexosidase, beta-acetylhexosaminidase, beta-acetylhexosaminidinase, beta-D-hexosaminidase, beta-D-N-acetylhexosaminidase, Beta-GlcNAcase, beta-glucosidase, beta-HEX, beta-HexNAcase, beta-hexosaminidase, beta-hexosaminidase A, beta-hexosaminidase B, beta-hexosaminidase I, beta-hexosaminidase II, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-hexosaminidase, beta-N-acetyl-hexosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, beta-N-acetylhexosaminidase A, beta-N-acetylhexosaminidase B, beta-Nacetylhexosaminidase S, beta-NAGase, beta-NAHA, Beta-NAHASE, bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase, BmCHI-h, BmGlcNAcase 1, BmGlcNAcase 2, BmNPV CHIA, CbsA, Chi-I, Chi-II, chitobiase, chitobiase 2, CpGV V-CHIA, dispersin B, DmHEXA, DmHEXB, DspB, EC 3.2.1.29, EC 3.2.1.30, endo-beta-N-acetylglucosaminidase, EXC1Y, EXC2Y, exo-acting chitinase, exo-beta-D-N-acetylglucosaminidase, exo-beta-hexosaminidase, exo-type beta-N-acetylhexosaminidase, exochitinase, F3F20.4 protein, FDL, GH84C, GlcNAcase, Hex, Hex A, Hex B, Hex-1, Hex1, Hex2, Hex20, Hex4, HexB, HexD, HEXDC, hEXO1, HEXO2, HEXO3, hexosaminidase, hexosaminidase A, hexosaminidase B, hexosaminidase D, hHexA, lacto-N-biosidase, lnba, lysosomal beta-hexosaminidase, More, N-acetyl-beta-D-hexosaminidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-hexosaminidase, N-acetylglucosaminidase, N-acetylhexosaminidase, NAG-68, NAG-B, NAG1, NAG2, Nag3, NagA, NAGase, NagJ, NagZ, NAHA, NAHase, neutral hexosaminidase C, O-GlcNAcase, Pcb-NAHA1, Sfhex, SSO3039, StrH, zymocin alpha-subunit

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.52 beta-N-acetylhexosaminidase

Temperature Stability

Temperature Stability on EC 3.2.1.52 - beta-N-acetylhexosaminidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
pH from 3.0-8.0, stable for 24 h
0 - 37
-
stable up to 24 h
20 - 40
-
1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide and p-nitrophenyl-N-acetyl-beta-D-galactosaminide is stable at
25
-
pH from 3.0-8.0, stable for 24 h
30 - 45
-
stable for 40 min
35 - 60
-
for 15 min at pH 7.0, gradual inactivation
37 - 42
-
isoform Am2301 retains more than 90% activity after 96 h of incubation at 37°C, and for 12 h incubation at 42°C. The activity of isoform Am2446 decreases already 90% within 2 h of incubation at 37°C
37 - 60
the enzyme is stable for at least 24 h at 37°C and 50°C. Preincubation of the enzyme at 60°C leads to its complete inactivation within 1 h
4 - 42
stable for 15 min, at pH 7.5
42
1 h, stable
45 - 50
the enzyme is thermo-stable up to 45°C, retaining 85.3% activity even at 50°C
48
-
Ex 1, stable below
5
-
stable at this temperature between pH 2.0-4.0
50 - 52
-
Hex A is labile, Hex B is stable, at lower sample concentrations Hex B is less stable at 52°C
55 - 60
the enzyme keeps over 80% activity after pre-incubation at 55°C for 1 h and 60% residual activity after 10 min pre-incubation at 60°C
56
-
at pH 4.5, loss of 30% of activity after 6 min
57.5
-
low molecular weight form EII, 50% of activity remains after 10 min
58
-
alimentary canal enzyme, 50% of activity remains
62
-
Ex 1, 50% of activity remains
85
about 45% loss of activity after 8 h, about 60% loss of activity after 24 h
90
-
complete inactivation after 10 min
additional information