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Literature summary for 3.2.1.52 extracted from
- Purification and properties of a beta-N-acteylaminoglucohydrolase from malted barley (1976), Phytochemistry, 15, 1869-1871.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable after lyophilization | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Hordeum vulgare |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
SDS-PAGE | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Hordeum vulgare | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley, malted | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 241fold, partial | Hordeum vulgare |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme solution unstable | Hordeum vulgare |
| frozen | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | - |
Hordeum vulgare | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Hordeum vulgare | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5 | - |
Hordeum vulgare |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | - |
Hordeum vulgare |
| 6 | - |
half-maximal activity | Hordeum vulgare |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
half-maximal activity | Hordeum vulgare |
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Release 2023.1 (January 2023)
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