Literature summary for 3.2.1.52 extracted from

Ficko-Blean, E.; Gregg, K.J.; Adams, J.J.; Hehemann, J.H.; Czjzek, M.; Smith, S.P.; Boraston, A.B.
Portrait of an enzyme, a complete structural analysis of a multimodular beta-N-acetylglucosaminidase from Clostridium perfringens (2009), J. Biol. Chem., 284, 9876-9884.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged enzyme fragments	Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme fragments: GH84C catalytic module fragment, and GH84C fragment comprising the catalytic module and the adjacent CBM32, as well as a fragment with the FN3 module, hanging drop vapor diffusion method, 10-25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with precipitant solution containing 0.1 M Tris-HCl, pH 7.5, 0.1 M MgCl2, 15% w/v PEG 4000, or 0.1 M sodium acetate, pH 3.5, 4% w/v PEG 8000, at 18°C, structure determination and analysis by combination of small angle X-ray scattering and X-ray crystallography at 1.8-3.3 A resolution	Clostridium perfringens

Crystallization (Comment)

Organism

purified recombinant enzyme fragments: GH84C catalytic module fragment, and GH84C fragment comprising the catalytic module and the adjacent CBM32, as well as a fragment with the FN3 module, hanging drop vapor diffusion method, 10-25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with precipitant solution containing 0.1 M Tris-HCl, pH 7.5, 0.1 M MgCl2, 15% w/v PEG 4000, or 0.1 M sodium acetate, pH 3.5, 4% w/v PEG 8000, at 18°C, structure determination and analysis by combination of small angle X-ray scattering and X-ray crystallography at 1.8-3.3 A resolution

Clostridium perfringens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of truncated enzyme mutants, GH84C fragments comprising the catalytic module only, or additionally the adjacent CBM32 or the FN3 modules	Clostridium perfringens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Clostridium perfringens	-	-

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	Q8XL08	gene nagJ	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme fragments	Clostridium perfringens

Subunits

Subunits	Comment	Organism
More	GH84C domain structure with the family 84 catalytic module, GH84C, a family 32 carbohydrate binding module,CBM, a cohesin module, Coh, and at the C-terminus of the enzyme a fibronectin type III module, FN3. Three-dimensional structure, domain orientations, analysis and modelling, overview	Clostridium perfringens

Synonyms

Synonyms	Comment	Organism
exo-beta-D-N-acetylglucosaminidase	-	Clostridium perfringens
GH84C	-	Clostridium perfringens
NagJ	-	Clostridium perfringens

General Information

General Information	Comment	Organism
physiological function	the enzyme is a large, extracellular carbohydrate-active virulence factor involved in host-pathogen interactions, overview. It is a 1001-amino acid multimodular homologue of the Clostridium perfringens mu-toxin	Clostridium perfringens