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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of N-acetyl-beta-D-hexosaminidase in different anatomical regions of the adult rat epididymis (1992), Biochem. Int., 28, 613-620.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-beta-D-glucosaminolactone | both isozymes | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caput, Hex A | Rattus norvegicus | |
| 0.61 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caput, Hex A | Rattus norvegicus | |
| 0.61 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caput, Hex B | Rattus norvegicus | |
| 0.63 | - |
4-nitrophenyl N-acetyl-beta-D-galactosaminide | caput, Hex B | Rattus norvegicus | |
| 0.66 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caudal, Hex A | Rattus norvegicus | |
| 0.68 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caudal, Hex A and Hex B | Rattus norvegicus | |
| 0.68 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caudal, Hex B | Rattus norvegicus | |
| 1.53 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | corpus, Hex A and Hex B | Rattus norvegicus | |
| 1.55 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | corpus, Hex A and Hex B | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE, Hex B | Rattus norvegicus |
| 63000 | - |
SDS-PAGE, Hex A | Rattus norvegicus |
| 223000 | - |
gel filtration, Hex B | Rattus norvegicus |
| 250000 | - |
gel filtration, Hex A | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Rattus norvegicus | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hex A: 161fold, Hex B: 189fold | Rattus norvegicus |
| N-acetylhexosaminidase: 63fold | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | same active site for N-glucosaminidase and N-galactosaminidase activities | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epididymis | from caput, corpus and caudal region of epididymis | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
Hex B | Rattus norvegicus |
| 2.8 | - |
Hex A | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Rattus norvegicus | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Rattus norvegicus | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Rattus norvegicus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Rattus norvegicus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.163 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caput, Hex B | Rattus norvegicus | |
| 0.208 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caput, Hex A | Rattus norvegicus | |
| 0.408 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caput, Hex B | Rattus norvegicus | |
| 0.45 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caput, Hex A | Rattus norvegicus | |
| 1.12 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caudal, Hex B | Rattus norvegicus | |
| 1.24 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | caudal, Hex A | Rattus norvegicus | |
| 1.38 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | corpus, Hex B | Rattus norvegicus | |
| 1.58 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | corpus, Hex A | Rattus norvegicus | |
| 1.63 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caudal, Hex B | Rattus norvegicus | |
| 1.93 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | caudal, Hex A | Rattus norvegicus | |
| 2.08 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | corpus, Hex B | Rattus norvegicus | |
| 2.66 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | corpus, Hex A | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
both isozymes | Rattus norvegicus |
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