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Literature summary for 3.2.1.52 extracted from

  • Berkeley, R.C.W.; Brewer, S.J.; Ortiz, J.M.; Gillespie, J.B.
    An exo-beta-N-acetylglucosaminidase from Bacillus subtilis B. Characterization (1973), Biochim. Biophys. Acta, 309, 157-168.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 and 20 mM causes 10% stimulation Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-2-acetamido-D-glucono(1-5)lactone
-
Bacillus sp. (in: Bacteria)
Ag+ 0.01 mM cause 30% inhibition, 2 mM cause 100% inhibition Bacillus sp. (in: Bacteria)
Ca2+
-
Bacillus sp. (in: Bacteria)
Cd2+ 2 mM cause 41% inhibition, 10 mM cause 59% inhibition Bacillus sp. (in: Bacteria)
Co2+ 10 mM cause 37% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 0.01 mM, 19% inhibition, 2 mM cause 97% inhibition Bacillus sp. (in: Bacteria)
Mg2+
-
Bacillus sp. (in: Bacteria)
N-acetyl-D-galactosamine
-
Bacillus sp. (in: Bacteria)
N-acetyl-D-glucosamine
-
Bacillus sp. (in: Bacteria)
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose substrate inhibition above 0.2 mM Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate 0.1 mM cause 16% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 2 mM cause 57% inhibition, 10 mM cause 78% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose
-
Bacillus sp. (in: Bacteria)
0.11
-
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
Bacillus sp. (in: Bacteria)
0.15
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Bacillus sp. (in: Bacteria)
0.31
-
N,N-diacetylchitobiose
-
Bacillus sp. (in: Bacteria)
0.35
-
N,N,N,N,N-pentaacetylchitopentaose
-
Bacillus sp. (in: Bacteria)
0.38
-
N,N',N'',N'''-tetraacetylchitotetraose
-
Bacillus sp. (in: Bacteria)
0.39
-
N,N',N''-triacetylchitotriose
-
Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Bacillus sp. (in: Bacteria)
75000
-
sucrose density gradient centrifugation, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bacillus sp. (in: Bacteria)
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
B. subtilis
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Bacillus sp. (in: Bacteria) 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
?
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
Bacillus sp. (in: Bacteria) 4 N-acetyl-D-glucosamine
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
Bacillus sp. (in: Bacteria) N-acetyl-D-glucosamine + N,N',N''-triacetylchitotriose
-
?
N,N',N''-triacetylchitotriose + H2O
-
Bacillus sp. (in: Bacteria) N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose
-
?
N,N'-diacetylchitobiose + H2O
-
Bacillus sp. (in: Bacteria) N-acetyl-D-glucosamine
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Bacillus sp. (in: Bacteria) N-acetyl-D-hexosamines
-
?
O-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-3-O-(D-carboxyethyl)-2-deoxy-D-glucose + H2O bacterial cell wall disaccharide from Micrococcus lysodeikticus Bacillus sp. (in: Bacteria) ?
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Bacillus sp. (in: Bacteria) 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
penta-N-acetylchitopentaose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
tri-N-acetylchitotriose + H2O
-
Bacillus sp. (in: Bacteria) N-acetyl-D-glucosamine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
-
Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
most stable at this pH, stability rapidly decreasing above or below Bacillus sp. (in: Bacteria)