Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol | - |
Saccharolobus solfataricus | |
conduritol beta-epoxide | - |
Saccharolobus solfataricus | |
N-butyldeoxynojirimycin | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus | |
0.9 | - |
4-methylumbelliferyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus | |
1.1 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
4.15 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
4.15 | - |
4-nitrophenyl beta-N-acetyl-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93400 | - |
3 * 93400, SDS-PAGE | Saccharolobus solfataricus |
93418 | - |
3 * 93418, calculated from sequence | Saccharolobus solfataricus |
313000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97UH8 | - |
- |
Saccharolobus solfataricus P2 | Q97UH8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.5 | - |
pH 4.0, 60°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | beta-D-galactose + 2-nitrophenol | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | beta-D-glucose + 2-nitrophenol | - |
? | |
4-methylumbelliferyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | beta-D-glucose + 4-methylumbelliferone | - |
? | |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Saccharolobus solfataricus | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Saccharolobus solfataricus | N-acetyl-beta-D-glucosamine + 4-methylumbelliferone | - |
? | |
4-nitrophenyl beta-D-galactose + H2O | - |
Saccharolobus solfataricus | beta-D-galactose + 4-nitrophenol | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | beta-D-glucose + 4-nitrophenol | - |
? | |
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Saccharolobus solfataricus | N-acetyl-beta-D-glucosamine + 4-nitrophenol | - |
? | |
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O | - |
Saccharolobus solfataricus | GlcNAc + ? | - |
? | |
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O | - |
Saccharolobus solfataricus | GlcNAc + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 93400, SDS-PAGE | Saccharolobus solfataricus |
trimer | 3 * 93418, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase | - |
Saccharolobus solfataricus |
SSO3039 | - |
Saccharolobus solfataricus |
SSO3039 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
70 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for 24 h, about 20% loss of activity after 48 h | Saccharolobus solfataricus |
85 | - |
about 45% loss of activity after 8 h, about 60% loss of activity after 24 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
8.8 | - |
4-methylumbelliferyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus | |
9.15 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus | |
19.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
19.2 | - |
4-nitrophenyl beta-N-acetyl-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | pH 3.0: about 70% of maximal activity, pH 5.0: about 70% of maximal activity | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00515 | - |
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol | pH 4.0, 60°C | Saccharolobus solfataricus | |
9 | - |
conduritol beta-epoxide | pH 4.0, 60°C | Saccharolobus solfataricus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00515 | - |
pH 7.0, 45°C | Saccharolobus solfataricus | N-butyldeoxynojirimycin | |
9 | - |
pH 7.0, 45°C | Saccharolobus solfataricus | conduritol beta-epoxide |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
1.6 | - |
4-nitrophenyl beta-N-acetyl-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
4.9 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | pH 4.0, 60°C | Saccharolobus solfataricus | |
9.9 | - |
4-methylumbelliferyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus | |
11.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.0, 60°C | Saccharolobus solfataricus |