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Literature summary for 3.2.1.52 extracted from

  • Ferrara, M.C.; Cobucci-Ponzano, B.; Carpentieri, A.; Henrissat, B.; Rossi, M.; Amoresano, A.; Moracci, M.
    The identification and molecular characterization of the first archaeal bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase demonstrate that family GH116 is made of three functionally distinct subfamilies (2014), Biochim. Biophys. Acta, 1840, 367-377.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol
-
Saccharolobus solfataricus
conduritol beta-epoxide
-
Saccharolobus solfataricus
N-butyldeoxynojirimycin
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
0.9
-
4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
1.1
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
4.15
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
4.15
-
4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93400
-
3 * 93400, SDS-PAGE Saccharolobus solfataricus
93418
-
3 * 93418, calculated from sequence Saccharolobus solfataricus
313000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UH8
-
-
Saccharolobus solfataricus P2 Q97UH8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
pH 4.0, 60°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Saccharolobus solfataricus beta-D-galactose + 2-nitrophenol
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Saccharolobus solfataricus beta-D-glucose + 2-nitrophenol
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
Saccharolobus solfataricus beta-D-glucose + 4-methylumbelliferone
-
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Saccharolobus solfataricus 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Saccharolobus solfataricus N-acetyl-beta-D-glucosamine + 4-methylumbelliferone
-
?
4-nitrophenyl beta-D-galactose + H2O
-
Saccharolobus solfataricus beta-D-galactose + 4-nitrophenol
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Saccharolobus solfataricus beta-D-glucose + 4-nitrophenol
-
?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
Saccharolobus solfataricus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Saccharolobus solfataricus N-acetyl-beta-D-glucosamine + 4-nitrophenol
-
?
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O
-
Saccharolobus solfataricus GlcNAc + ?
-
?
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + H2O
-
Saccharolobus solfataricus GlcNAc + ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 93400, SDS-PAGE Saccharolobus solfataricus
trimer 3 * 93418, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase
-
Saccharolobus solfataricus
SSO3039
-
Saccharolobus solfataricus
SSO3039 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus
70
-
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
stable for 24 h, about 20% loss of activity after 48 h Saccharolobus solfataricus
85
-
about 45% loss of activity after 8 h, about 60% loss of activity after 24 h Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
8.8
-
4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
9.15
-
4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
19.2
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
19.2
-
4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3.0: about 70% of maximal activity, pH 5.0: about 70% of maximal activity Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00515
-
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol pH 4.0, 60°C Saccharolobus solfataricus
9
-
conduritol beta-epoxide pH 4.0, 60°C Saccharolobus solfataricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00515
-
pH 7.0, 45°C Saccharolobus solfataricus N-butyldeoxynojirimycin
9
-
pH 7.0, 45°C Saccharolobus solfataricus conduritol beta-epoxide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
1.6
-
4-nitrophenyl beta-N-acetyl-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
4.9
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide pH 4.0, 60°C Saccharolobus solfataricus
9.9
-
4-methylumbelliferyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus
11.4
-
4-nitrophenyl beta-D-glucopyranoside pH 4.0, 60°C Saccharolobus solfataricus