Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.0075 mM: activity reaches 110.0% | Bombyx mori |
General Stability | Organism |
---|---|
lyophilization reduces activity to 34.5% | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | with 0.03 mM 42.9% of activity remains | Bombyx mori | |
Hg2+ | with 0.03 mM 7.1% of activity remains | Bombyx mori | |
Mg2+ | with 0.03 mM 71.4% of activity remains | Bombyx mori | |
additional information | high substrate concentrations, i.e. concentrations above 0.2 mM | Bombyx mori | |
Zn2+ | with 0.03 mM 88.6% of activity remains | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
(N-acetyl-beta-D-glucosamine)6 | - |
Bombyx mori | |
0.09 | - |
(N-acetyl-beta-D-glucosamine)5 | - |
Bombyx mori | |
0.118 | - |
(N-acetyl-beta-D-glucosamine)4 | - |
Bombyx mori | |
0.122 | - |
(N-acetyl-beta-D-glucosamine)3 | - |
Bombyx mori | |
0.397 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Bombyx mori | |
0.433 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Bombyx mori | |
0.787 | - |
(N-acetyl-beta-D-glucosamine)2 | - |
Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE | Bombyx mori |
67500 | - |
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE | Bombyx mori |
67500 | - |
Ex 1, homodimer: 2 * 67500 | Bombyx mori |
119000 | - |
gel filtration | Bombyx mori |
125000 | - |
SDS-PAGE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Bombyx mori | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
5-day old pupae | - |
Purification (Comment) | Organism |
---|---|
64fold to homogeneity | Bombyx mori |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alimentary canal | - |
Bombyx mori | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Bombyx mori |
Storage Stability | Organism |
---|---|
-20°C, after 27 days 87.1% of activity remains | Bombyx mori |
4°C, not stable, rapid decrease of activity after the first 12 days | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(N-acetyl-beta-D-glucosamine)2 + H2O | - |
Bombyx mori | 2 N-acetyl-beta-D-glucosamine | - |
? | |
(N-acetyl-beta-D-glucosamine)3 + H2O | - |
Bombyx mori | (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine | - |
? | |
(N-acetyl-beta-D-glucosamine)4 + H2O | - |
Bombyx mori | (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine | - |
? | |
(N-acetyl-beta-D-glucosamine)5 + H2O | - |
Bombyx mori | (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine | - |
? | |
(N-acetyl-beta-D-glucosamine)6 + H2O | - |
Bombyx mori | (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine | - |
? | |
chitin + H2O | colloidal chitin | Bombyx mori | N-acetylglucosamine | - |
? | |
chitin oligosaccharides + H2O | (N-acetyl-D-glucosamine)2-6 | Bombyx mori | N-acetyl-D-glucosamine | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | - |
Bombyx mori | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Bombyx mori | ? | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Bombyx mori | 2 N,N-diacetylchitobiose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Bombyx mori | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bombyx mori | deacetylated chitooligosaccharides + acetate | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Bombyx mori | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Bombyx mori | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Bombyx mori | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE | Bombyx mori |
dimer | Ex 1, homodimer: 2 * 67500 | Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
alimentary canal enzyme | Bombyx mori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable below, for 10 min | Bombyx mori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.9 | - |
(N-acetyl-beta-D-glucosamine)6 | - |
Bombyx mori | |
75.6 | - |
(N-acetyl-beta-D-glucosamine)5 | - |
Bombyx mori | |
129 | - |
(N-acetyl-beta-D-glucosamine)4 | - |
Bombyx mori | |
209 | - |
(N-acetyl-beta-D-glucosamine)3 | - |
Bombyx mori | |
820 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Bombyx mori | |
1010 | - |
(N-acetyl-beta-D-glucosamine)2 | - |
Bombyx mori | |
1150 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Bombyx mori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | stable | Bombyx mori |