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(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
?
-
-
-
-
?
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
?
-
-
-
-
?
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
pyruvate + (2R,3S)-2,3-dihydroxy-4-oxo-N,N-dipropylbutanamide
-
-
-
-
r
2-deoxy-2-[[(alpha-D-galactopyranosyloxy)acetyl]amino]-D-mannopyranose + pyruvate
3,5-dideoxy-5-[[(alpha-D-galactopyranosyloxy)acetyl]amino]-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
36% yield
-
?
2-deoxy-2-[[(beta-D-galactopyranosyloxy)acetyl]amino]-D-mannopyranose + pyruvate
3,5-dideoxy-5-[[(beta-D-galactopyranosyloxy)acetyl]amino]-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
34% yield
-
?
2-deoxy-D-glucose + pyruvate
?
3-bromo-3-deoxy-D-mannose + pyruvate
?
-
wild-type enzyme: 5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 10% of the activity with N-acetyl-D-mannosamine
-
-
?
3-deoxy-manno-oct-ulosonic acid
arabinose + pyruvate
-
-
-
?
4-O-alpha-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
5% yield
-
?
4-O-beta-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-beta-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
38% yield
-
?
4-O-beta-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-beta-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
35% yield
-
?
5-N-acetyl-4-O-acetylneuraminic acid
?
-
22% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
5-O-alpha-D-galactopyranosyl-alpha-D-mannofuranose + pyruvate
3-deoxy-8-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
62% yield
-
?
5-O-beta-D-galactopyranosyl-alpha-D-mannofuranose + pyruvate
3-deoxy-8-O-beta-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
85% yield
-
?
5-O-methyl-N-acetyl-D-mannosamine + pyruvate
8-O-methyl-N-acetylneuraminate
-
-
-
?
6-O-alpha-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
81% yield
-
?
6-O-alpha-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
65% yield
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-mannopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
78% yield
-
?
6-O-beta-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-beta-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
83% yield
-
?
D-3-deoxy-manno-2-octulosonic acid
D-arabinose + pyruvate
-
-
-
-
r
D-arabinose + pyruvate
3-deoxy-D-manno-2-octulosonic acid
D-arabinose + pyruvate
D-3-deoxy-manno-2-octulosonic acid
-
wild-type enzyme: 20% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 65% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 85% of the activity with N-acetyl-D-mannosamine
-
-
r
D-glucose + pyruvate
?
-
wild-type enzyme: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 20% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 25% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 20% of the activity with N-acetyl-D-mannosamine
-
-
?
D-gulose + pyruvate
?
-
wild-type enzyme: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 110% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 90% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 70% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannosamine + pyruvate
?
-
wild-type enzyme: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 90% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 165% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 190% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 170% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannose + pyruvic acid
2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid
-
-
-
-
?
D-talose + pyruvate
?
-
wild-type enzyme: 60% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 50% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 105% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 70% of the activity with N-acetyl-D-mannosamine
-
-
?
L-3-deoxy-manno-2-octulosonic acid
L-arabinose + pyruvate
-
-
-
-
r
L-arabinose + ?
3-deoxy-L-manno-oct-2-ulosonic acid
-
-
?
L-arabinose + pyruvate
L-3-deoxy-manno-2-octulosonic acid
-
wild-type enzyme: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 35% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 80% of the activity with N-acetyl-D-mannosamine
-
-
r
L-mannose + pyruvate
?
-
wild-type enzyme: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 110% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 95% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 95% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 95% of the activity with N-acetyl-D-mannosamine
-
-
?
Methoxyneuraminic acid
?
-
-
-
-
?
N,O-Diacetylneuramininic acid
?
-
-
-
-
?
N-Acetyl-4-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-7-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-8-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-9-O-acetylneuraminic acid
?
-
-
-
-
?
N-acetyl-D-mannosamine + pyruvate
N-acetyl-D-neuraminic acid
-
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
N-acetyl-D-mannosamine + pyruvic acid
N-acetyl-D-neuraminic acid
-
-
-
-
?
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
N-acetyl-D-neuraminic acid
pyruvate + N-acetyl-D-mannosamine
N-acetyl-L-mannosamine + pyruvate
N-acetyl-L-neuraminic acid
-
wild-type enzyme: 0.2% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 0.5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 1% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 2% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 2% of the activity with N-acetyl-D-mannosamine
-
-
r
N-acetyl-L-neuraminic acid
N-acetyl-L-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
N-Acetylmannosamine + pyruvate
?
N-acetylmannosamine + pyruvate
N-acetylneuraminic acid
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
N-acetylneuraminic acid
N-acetylmannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminic acid
pyruvate + N-acetyl-D-mannosamine
N-Formylneuraminic acid
?
N-Glycolylneuraminic acid
?
N-Monochloracetylneuraminic acid
?
N-Monofluoracetylneuraminic acid
?
-
-
-
-
?
N-Succinylneuraminic acid
?
-
-
-
-
?
pyruvate + alpha-D-Galp-(1,6)-D-Manp
alpha-D-Galp-(1,9)-D-deoxyneuraminic acid
-
-
-
-
?
additional information
?
-
2-deoxy-D-glucose + pyruvate

?
-
33% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
-
33% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
1.2% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
1.2% activity compared to N-acetylneuraminate
-
-
r
5-N-acetyl-9-O-acetylneuraminic acid

?
-
54% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
-
32.45% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
-
45% the activity with N-acetylneuraminic acid
-
?
D-arabinose + pyruvate

3-deoxy-D-manno-2-octulosonic acid
-
1.0% activity compared to N-acetylneuraminate
-
-
r
D-arabinose + pyruvate
3-deoxy-D-manno-2-octulosonic acid
1.5% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate

?
-
wild-type enzyme: 30% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 75% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannose + pyruvate
?
-
95% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
-
95% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
10% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
10% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate

?
-
wild-type enzyme: 45% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 55% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 25% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 25% of the activity with N-acetyl-D-mannosamine
-
-
?
D-xylose + pyruvate
?
-
2.4% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
-
2.4% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
0.6% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
0.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate

?
-
wild-type enzyme: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 35% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 15% of the activity with N-acetyl-D-mannosamine
-
-
?
L-gulose + pyruvate
?
-
3.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
-
3.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
0.7% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
0.7% activity compared to N-acetylneuraminate
-
-
r
N-acetyl-D-mannosamine + pyruvate

N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine
-
-
r
N-Acetyl-D-mannosamine + pyruvate

N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-acetyl-D-neuraminic acid

N-acetyl-D-mannosamine + pyruvate
-
in presence of excess pyruvate the equilibrium may be driven in the direction of aldol product formation
-
-
r
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetyl-D-neuraminic acid

pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-acetyl-D-neuraminic acid
pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-acetyl-neuraminic acid

N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylmannosamine + pyruvate

?
-
-
-
-
?
N-Acetylmannosamine + pyruvate
?
-
N-acetylneuraminic acid is intermediate for colomic acid biosynthesis
-
-
?
N-acetylneuraminate

N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
the enzyme catalyzes the reversible condensation of pyruvate with N-acetyl-D-mannosamine to yield the sialic acid N-acetylneuraminic acid
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
reversible aldol cleavage of N-acetylneuraminic acid
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylneuraminic acid

?
Corynebacterium belfanti
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
N-acetylneuraminate lyase plays important role in regulation of N-acetylneuraminic acid metabolism
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid

N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
Corynebacterium belfanti
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
syn: Neu5Ac
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
formation of a Schiff base
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
regulation of sialic acid metabolism
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminic acid

pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-acetylneuraminic acid
pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-Formylneuraminic acid

?
-
-
-
-
?
N-Formylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid

?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
60% the rate of N-acetylneuraminic acid
-
?
N-Glycolylneuraminic acid
?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid
?
-
47% the activity with N-acetylneuraminic acid
-
?
N-Monochloracetylneuraminic acid

?
-
-
-
-
?
N-Monochloracetylneuraminic acid
?
-
-
-
?
additional information

?
-
-
structural characteristics of substrates
-
-
?
additional information
?
-
-
configuration of substrate and products
-
-
?
additional information
?
-
-
condensation: N-acetylglucosamine, N-acetylgalactosamine, glucosamine and galactosamine cannot replace N-acetylmannosamine. Phosphoenolpyruvate, lactate, beta-hydroxypyruvate and other pyruvate derivatives cannot replace pyruvate
-
-
?
additional information
?
-
-
no substrates are 3-azido-3-deoxy, 3-amino-3-deoxy and 3-N-tert-butylcarbonyl-3-deoxy derivatives of 2-acetamido-2-deoxy-alpha,beta-D-mannose
-
?
additional information
?
-
One point mutation (L142R) is sufficient to create an enzyme that can complement a bacterial auxotroph lacking the gene for dihydrodipicolinate synthase, EC 4.2.1.52. The mutant enzyme catalyzes the reaction: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O
-
?
additional information
?
-
-
One point mutation (L142R) is sufficient to create an enzyme that can complement a bacterial auxotroph lacking the gene for dihydrodipicolinate synthase, EC 4.2.1.52. The mutant enzyme catalyzes the reaction: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O
-
?
additional information
?
-
-
not: N-acetyl-4-O-acetylneuraminic acid and 2-deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
?
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0.39 - 11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.35 - 10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.2 - 11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
3.5 - 14.5
D-3-deoxy-manno-2-octulosonic acid
2.4 - 14.1
L-3-deoxy-manno-2-octulosonic acid
12.5
N-acetyl-4-O-acetylneuraminic acid
-
-
4.5
N-Acetyl-7-O-acetylneuraminic acid
-
-
2
N-Acetyl-8-O-acetylneuraminic acid
-
-
6.9 - 3200
N-acetyl-D-mannosamine
2.1 - 38
N-acetyl-D-neuraminic acid
3 - 369.4
N-acetyl-L-neuraminic acid
2.8
N-acetyl-neuraminic acid
-
in 0.1 M phosphate buffer, pH 7
0.013 - 87.7
N-acetylneuraminate
0.4 - 1100
N-acetylneuraminic acid
1.75
N-Formylneuraminic acid
-
-
2.2 - 4.6
N-glycolylneuraminic acid
2.7
N-Monochloracetylneuraminic acid
-
-
10
N-Monofluoracetylneuraminic acid
-
-
1.93
N-Succinylneuraminic acid
-
-
additional information
additional information
-
with substrate N-acetylneuraminic acid increasing Km with increasing pH
-
0.39
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide

-
mutant enzyme E192N
11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
wild-type enzyme
0.35
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid

-
pH 7.5, 25ưC, mutant enzyme E192V
0.39
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme E192N
0.5
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme E192S
2.54
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme S208G
5.7
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme D191P
10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, wild-type enzyme
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid

-
E192M mutant protein, pH 7.5, 25ưC
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25ưC
0.3
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25ưC
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25ưC
0.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25ưC
0.6
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25ưC
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25ưC
1.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192D mutant protein, pH 7.5, 25ưC
11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25ưC
3.5
D-3-deoxy-manno-2-octulosonic acid

-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
6.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
11.4
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
12.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L
13.8
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, wild-type enzyme
14.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
2.4
L-3-deoxy-manno-2-octulosonic acid

-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
2.7
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
2.8
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
7.5
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, wild-type enzyme
9.2
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
14.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L
6.9
N-acetyl-D-mannosamine

at pH 7.0 and 37ưC
7.7
N-acetyl-D-mannosamine
-
-
53.3
N-acetyl-D-mannosamine
at pH 7.5 and 37ưC
92.1
N-acetyl-D-mannosamine
at pH 8.5 and 37ưC
131.7
N-acetyl-D-mannosamine
-
recombinant enzyme, pH and temperature not specified in the publication
149
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37ưC
160
N-acetyl-D-mannosamine
-
pH 7.0, 37ưC
160
N-acetyl-D-mannosamine
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
180
N-acetyl-D-mannosamine
-
220
N-acetyl-D-mannosamine
-
245
N-acetyl-D-mannosamine
-
cross-linked enzyme aggregates, pH 7.5, 37ưC
272
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37ưC
333
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37ưC
915
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37ưC
3200
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
2.1
N-acetyl-D-neuraminic acid

-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L
2.6
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, wild-type enzyme
3.9
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
4.4
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, wild-type enzyme
10.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme S208G
12.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
15.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
17
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192S
34
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192V
38
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192N
3 - 3.5
N-acetyl-L-neuraminic acid

-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
4.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, wild-type enzyme
36.2
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
127
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
369.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
0.013
N-acetylneuraminate

purified recombinant mutant K165C, pH 7.4, 30ưC
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30ưC
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30ưC
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30ưC
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30ưC
0.27
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37ưC
0.32
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37ưC
1.1
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37ưC
1.2
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37ưC
1.8
N-acetylneuraminate
at pH 7.0 and 37ưC
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37ưC
2
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
2.2
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30ưC
2.5
N-acetylneuraminate
-
2.51
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
3 - 3.5
N-acetylneuraminate
at pH 7.5 and 37ưC
3.2
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
3.2
N-acetylneuraminate
at 30ưC, pH not specified in the publication
3.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
3.42
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
3.7
N-acetylneuraminate
-
mutant Y137F enzyme, pH 7.4, 30ưC
3.93
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4
N-acetylneuraminate
-
recombinant enzyme, pH and temperature not specified in the publication
4.1
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4.3
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4.7
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4.9
N-acetylneuraminate
-
5
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
6.1
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
87.7
N-acetylneuraminate
at pH 8.5 and 37ưC
0.4
N-acetylneuraminic acid

-
pH 7.2, Y133F mutant
1
N-acetylneuraminic acid
-
pH 7.2, Y133H mutant
1
N-acetylneuraminic acid
-
pH 7.4, 75ưC
1.4
N-acetylneuraminic acid
-
pH 7.4, 37ưC
1.5 - 3.7
N-acetylneuraminic acid
-
-
1.7
N-acetylneuraminic acid
-
pH 7.4, 37ưC
1.75
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
pH 7.0, 37ưC
1.9 - 3.9
N-acetylneuraminic acid
-
-
2
N-acetylneuraminic acid
-
pH 7.2, 50 mM phosphate buffer, wild-type
2.4
N-acetylneuraminic acid
-
-
2.4
N-acetylneuraminic acid
-
pH 7.2, Y133C mutant
2.8
N-acetylneuraminic acid
-
-
2.9
N-acetylneuraminic acid
-
pH 7.2, Y133W mutant
3.2
N-acetylneuraminic acid
-
pH 7.2, 250 mM phosphate buffer, wild-type
3.3
N-acetylneuraminic acid
-
-
3.3
N-acetylneuraminic acid
-
-
3.5
N-acetylneuraminic acid
-
pH 7.2, K161R mutant
3.5
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25ưC
3.6
N-acetylneuraminic acid
-
-
3.7
N-acetylneuraminic acid
-
-
3.9
N-acetylneuraminic acid
-
-
4.4
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25ưC
4.6
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25ưC
4.8
N-acetylneuraminic acid
-
-
5
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25ưC
5.9
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25ưC
6
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25ưC
6
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25ưC
6.2
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25ưC
17
N-acetylneuraminic acid
-
pH 7.2, E188Q mutant
29
N-acetylneuraminic acid
-
pH 7.2, D187E mutant
34
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25ưC
38
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
44
N-acetylneuraminic acid
-
pH 7.2, E188D mutant
1100
N-acetylneuraminic acid
-
pH 7.2, D187N mutant
2.2
N-glycolylneuraminic acid

-
-
3.3
N-glycolylneuraminic acid
-
-
4.3
N-glycolylneuraminic acid
-
-
4.6
N-glycolylneuraminic acid
-
-
0.5
pyruvate

mutant enzyme P193Y, at pH 7.0 and 37ưC
1 - 4
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37ưC
1.1
pyruvate
wild type enzyme, at pH 7.0 and 37ưC
4.3
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
5.9
pyruvate
at pH 7.0 and 37ưC
9
pyruvate
-
cross-linked enzyme aggregates, pH 7.5, 37ưC
12.5
pyruvate
-
wild type enzyme, at pH 7.0 and 37ưC
14.7
pyruvate
at pH 7.5 and 37ưC
19.9
pyruvate
-
pH 7.0, 37ưC
19.9
pyruvate
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
35.1
pyruvate
-
recombinant enzyme, pH and temperature not specified in the publication
72.4
pyruvate
at pH 8.5 and 37ưC
4.4
sialic acid

-
wild-type enzyme
38
sialic acid
-
mutant enzyme E192N
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.057 - 10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.44 - 2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.1 - 2.83
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
1.2 - 8.9
D-3-deoxy-manno-2-octulosonic acid
1.1 - 10.4
L-3-deoxy-manno-2-octulosonic acid
2.4 - 40.67
N-acetyl-D-mannosamine
1.5 - 10.5
N-acetyl-D-neuraminic acid
0.0002 - 0.02
N-acetyl-L-neuraminic acid
0.013 - 44.2
N-acetylneuraminate
0.42 - 16.7
N-acetylneuraminic acid
0.057 - 0.65
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide

-
mutant enzyme E192N
1.23
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
wild-type enzyme
10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
mutant enzyme E192N
0.44
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid

-
pH 7.5, 25ưC, mutant enzyme S208G
0.91
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme D191P
1.22
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, wild-type enzyme
1.58
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme E192V
1.85
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme E192S
2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25ưC, mutant enzyme E192N
0.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid

-
E192D mutant protein, pH 7.5, 25ưC
0.43
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25ưC
0.63
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25ưC
0.82
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25ưC
1.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25ưC
1.22
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25ưC
1.47
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25ưC
1.58
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25ưC
2.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
2.83
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25ưC
1.2
D-3-deoxy-manno-2-octulosonic acid

-
pH 7.5, 25ưC, wild-type enzyme
1.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L
2.8
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
3.2
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
5.1
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
8.9
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.1
L-3-deoxy-manno-2-octulosonic acid

-
pH 7.5, 25ưC, wild-type enzyme
1.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L
1.9
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
2.5
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
4.2
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.4
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
2.4
N-acetyl-D-mannosamine

mutant enzyme P193Y, at pH 7.0 and 37ưC
4.5
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37ưC
4.6
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37ưC
5.4
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37ưC
5.67
N-acetyl-D-mannosamine
at pH 7.5 and 37ưC
9
N-acetyl-D-mannosamine
-
11
N-acetyl-D-mannosamine
-
15.3
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
40.67
N-acetyl-D-mannosamine
at pH 8.5 and 37ưC
1.5
N-acetyl-D-neuraminic acid

-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
1.97
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192V
2.2
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
2.77
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192N
4.15
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme S208G
4.25
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E192S
4.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, wild-type enzyme
9.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
10.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, Y98H/F115L
10.5
N-acetyl-D-neuraminic acid
-
pH 7.5, 25ưC, wild-type enzyme
0.0002
N-acetyl-L-neuraminic acid

-
pH 7.5, 25ưC, wild-type enzyme
0.0045
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I
0.0068
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/V251I/V265I
0.02
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
0.02
N-acetyl-L-neuraminic acid
-
pH 7.5, 25ưC, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
0.013
N-acetylneuraminate

-
mutant Y137F enzyme, pH 7.4, 30ưC
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30ưC
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30ưC
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30ưC
0.032
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37ưC
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30ưC
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30ưC
0.13
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37ưC
0.29
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
0.47
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
0.664
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
0.9
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37ưC
1.9
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37ưC
3.15
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
4
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37ưC
4.17
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30ưC
4.69
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
5.49
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
6.75
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
7.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
7.72
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
7.94
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
8.9
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
9.3
N-acetylneuraminate
at pH 7.5 and 37ưC
11.87
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30ưC
22.1
N-acetylneuraminate
at 30ưC, pH not specified in the publication
44.2
N-acetylneuraminate
at pH 8.5 and 37ưC
0.42
N-acetylneuraminic acid

-
E192P mutant protein, pH 7.5, 25ưC
0.83
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25ưC
1.17
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25ưC
2
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25ưC
2
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25ưC
2
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25ưC
2.67
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25ưC
2.83
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
4.33
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25ưC
5
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25ưC
6.8
N-acetylneuraminic acid
pH 7.2, 25ưC, L142R/Y190D/E192A mutant
7.7
N-acetylneuraminic acid
pH 7.2, 25ưC, wild-type
12.1
N-acetylneuraminic acid
pH 7.2, 25ưC, L142R mutant
16.7
N-acetylneuraminic acid
-
-
0.99
pyruvate

mutant enzyme P193Y, at pH 7.0 and 37ưC
2.9
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37ưC
3
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
3.9
pyruvate
-
wild type enzyme, at pH 7.0 and 37ưC
5.6
pyruvate
wild type enzyme, at pH 7.0 and 37ưC
6.1
pyruvate
at pH 7.5 and 37ưC
42.58
pyruvate
at pH 8.5 and 37ưC
2.833
sialic acid

-
mutant enzyme E192N
3 - 6
sialic acid
-
mutant enzyme E192N
4.33
sialic acid
-
wild-type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.08 - 5.67
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
0.002 - 1.86
N-acetyl-D-mannosamine
0.0033 - 23.75
N-acetylneuraminate
0.06 - 5.6
N-acetylneuraminic acid
0.08
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid

-
E192D mutant protein, pH 7.5, 25ưC
0.12
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25ưC
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25ưC
1.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25ưC
4.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25ưC
4.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25ưC
4.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25ưC
5.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25ưC
5.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25ưC
5.67
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
0.002
N-acetyl-D-mannosamine

mutant enzyme P193Y, at pH 7.0 and 37ưC
0.004
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
0.0074
N-acetyl-D-mannosamine
-
cross-linked enzyme aggregates, pH 7.5, 37ưC
0.016
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37ưC
0.016
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37ưC
0.03
N-acetyl-D-mannosamine
-
pH 7.0, 37ưC
0.03
N-acetyl-D-mannosamine
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
0.03
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37ưC
0.07
N-acetyl-D-mannosamine
at pH 8.5 and 37ưC
0.08
N-acetyl-D-mannosamine
-
recombinant enzyme, pH and temperature not specified in the publication
0.11
N-acetyl-D-mannosamine
at pH 7.5 and 37ưC
1.86
N-acetyl-D-mannosamine
at pH 7.0 and 37ưC
0.0033
N-acetylneuraminate

-
mutant Y137F enzyme, pH 7.4, 30ưC
0.026
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37ưC
0.23
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
0.28
N-acetylneuraminate
at pH 7.5 and 37ưC
0.48
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37ưC
1.7
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37ưC
1.9
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30ưC
1.9
N-acetylneuraminate
-
recombinant enzyme, pH and temperature not specified in the publication
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37ưC
2.8
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37ưC
3.66
N-acetylneuraminate
at pH 7.0 and 37ưC
23.75
N-acetylneuraminate
at pH 8.5 and 37ưC
0.06
N-acetylneuraminic acid

-
E192V mutant protein, pH 7.5, 25ưC
0.07
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25ưC
0.07
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25ưC
0.13
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25ưC
0.23
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25ưC
0.32
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25ưC
0.43
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25ưC
0.45
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25ưC
0.98
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25ưC
1.42
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25ưC
5.6
N-acetylneuraminic acid
-
pH 7.0, 37ưC
0.08
pyruvate

-
recombinant enzyme, pH and temperature not specified in the publication
0.09
pyruvate
-
cross-linked enzyme aggregates, pH 7.5, 37ưC
0.11
pyruvate
-
pH 7.0, 37ưC
0.11
pyruvate
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
0.11
pyruvate
at pH 8.5 and 37ưC
0.21
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37ưC
0.31
pyruvate
-
wild type enzyme, at pH 7.0 and 37ưC
0.41
pyruvate
at pH 7.5 and 37ưC
0.71
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37ưC
1.12
pyruvate
at pH 7.0 and 37ưC
1.98
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37ưC
5.1
pyruvate
wild type enzyme, at pH 7.0 and 37ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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