Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | about 120% activity at 5 mM | Mycoplasma sp. | |
TritonX-100 | about 120% activity at 5 mM | Mycoplasma sp. |
Application | Comment | Organism |
---|---|---|
industry | the enzyme has potential in the industrial production of N-acetylneuraminic acid | Mycoplasma sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycoplasma sp. |
Protein Variants | Comment | Organism |
---|---|---|
W189A | the kcat/Km value of the mutant is about 3fold higher than those of the wild type enzyme | Mycoplasma sp. |
W189F | the kcat/Km value of the mutant is about 10fold higher than those of the wild type enzyme | Mycoplasma sp. |
W189Y | the kcat/Km value of the mutant is about 7fold higher than those of the wild type enzyme | Mycoplasma sp. |
General Stability | Organism |
---|---|
enzyme cleavage activity is measured as 90% of its original activity at 5 MPa and 80% of the activity at 10 MPa | Mycoplasma sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethylammonium bromide | 40% inhibition at 5 mM | Mycoplasma sp. | |
Fe3+ | complete inhibition at 5 mM | Mycoplasma sp. | |
SDS | 20% inhibition at 5 mM | Mycoplasma sp. | |
Zn2+ | 30% inhibition at 5 mM | Mycoplasma sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
N-acetylneuraminate | at pH 7.0 and 37°C | Mycoplasma sp. | |
5.9 | - |
pyruvate | at pH 7.0 and 37°C | Mycoplasma sp. | |
6.9 | - |
N-acetyl-D-mannosamine | at pH 7.0 and 37°C | Mycoplasma sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 115% activity at 5 mM | Mycoplasma sp. | |
K+ | about 115% activity at 5 mM | Mycoplasma sp. | |
additional information | not influenced by Na+ and Mg2+ | Mycoplasma sp. | |
Ni2+ | about 110% activity at 5 mM | Mycoplasma sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminate | Mycoplasma sp. | - |
N-acetyl-D-mannosamine + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma sp. | A0A2P1G7E2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + pyruvate | - |
Mycoplasma sp. | N-acetylneuraminate | - |
r | |
N-acetylneuraminate | - |
Mycoplasma sp. | N-acetyl-D-mannosamine + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Mycoplasma sp. |
Synonyms | Comment | Organism |
---|---|---|
N-Acetylneuraminate lyase | - |
Mycoplasma sp. |
NAL | - |
Mycoplasma sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
for N-acetylneuraminate synthesis | Mycoplasma sp. |
70 | - |
for N-acetylneuraminate cleavage | Mycoplasma sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 70 | the enzyme remains stable after incubation for 150 min at 37°C. When incubated at 70°C, the half-life for enzymatic activity is 30 min and that for a complete loss of activity is 1 h | Mycoplasma sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | for N-acetylneuraminate synthesis | Mycoplasma sp. |
7 | - |
for N-acetylneuraminate cleavage | Mycoplasma sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme maintains around 95% residual cleavage activity after 120 min incubation at pH 6.0, and 85% residual cleavage activity after 120 min incubation at pH 8.0 | Mycoplasma sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
pyruvate | at pH 7.0 and 37°C | Mycoplasma sp. | |
1.86 | - |
N-acetyl-D-mannosamine | at pH 7.0 and 37°C | Mycoplasma sp. | |
3.66 | - |
N-acetylneuraminate | at pH 7.0 and 37°C | Mycoplasma sp. |