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Literature summary for 4.1.3.3 extracted from

  • Sanchez-Carron, G.; Garcia-Garcia, M.I.; Lopez-Rodriguez, A.B.; Jimenez-Garcia, S.; Sola-Carvajal, A.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    Molecular characterization of a novel N-acetylneuraminate lyase from Lactobacillus plantarum WCFS1 (2011), Appl. Environ. Microbiol., 77, 2471-2478.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3)pLys Lactiplantibacillus plantarum

General Stability

General Stability Organism
5-10% activity in the synthetic direction above pH 11.0 Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
N-acetylneuraminic acid pH 7.0, 37°C Lactiplantibacillus plantarum
19.9
-
pyruvate pH 7.0, 37°C Lactiplantibacillus plantarum
160
-
N-acetyl-D-mannosamine pH 7.0, 37°C Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37317
-
4 * 37317, HPLC/ESI/ion trap Lactiplantibacillus plantarum
132000
-
gel filtration Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
100-kDa ultrafiltration step, 65°C heat shock step and Ni2+ chelating affinity chromatography Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.65
-
pH 7.0, 37°C Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetylneuraminic acid
-
Lactiplantibacillus plantarum pyruvate + N-acetyl-D-mannosamine
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 37317, HPLC/ESI/ion trap Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
N-Acetylneuraminate lyase
-
Lactiplantibacillus plantarum
Sialic acid aldolase
-
Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
synthesis of N-acetylneuraminic acid Lactiplantibacillus plantarum
70
-
cleavage activity Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
80% cleavage activity after 48 hours Lactiplantibacillus plantarum
70
-
less than 10% after 8 hours Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.3 for both directions Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
5 9 for both directions Lactiplantibacillus plantarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11 about 80% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 8.0-10.0 and 37°C, 60% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 11.0 and 37°C Lactiplantibacillus plantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
N-acetyl-D-mannosamine pH 7.0, 37°C Lactiplantibacillus plantarum
0.11
-
pyruvate pH 7.0, 37°C Lactiplantibacillus plantarum
5.6
-
N-acetylneuraminic acid pH 7.0, 37°C Lactiplantibacillus plantarum