Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3)pLys | Lactiplantibacillus plantarum |
General Stability | Organism |
---|---|
5-10% activity in the synthetic direction above pH 11.0 | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
N-acetylneuraminic acid | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
19.9 | - |
pyruvate | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
160 | - |
N-acetyl-D-mannosamine | pH 7.0, 37°C | Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37317 | - |
4 * 37317, HPLC/ESI/ion trap | Lactiplantibacillus plantarum |
132000 | - |
gel filtration | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
100-kDa ultrafiltration step, 65°C heat shock step and Ni2+ chelating affinity chromatography | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.65 | - |
pH 7.0, 37°C | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminic acid | - |
Lactiplantibacillus plantarum | pyruvate + N-acetyl-D-mannosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37317, HPLC/ESI/ion trap | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
N-Acetylneuraminate lyase | - |
Lactiplantibacillus plantarum |
Sialic acid aldolase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
synthesis of N-acetylneuraminic acid | Lactiplantibacillus plantarum |
70 | - |
cleavage activity | Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
80% cleavage activity after 48 hours | Lactiplantibacillus plantarum |
70 | - |
less than 10% after 8 hours | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.3 | for both directions | Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | for both directions | Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | about 80% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 8.0-10.0 and 37°C, 60% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 11.0 and 37°C | Lactiplantibacillus plantarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-acetyl-D-mannosamine | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
0.11 | - |
pyruvate | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
5.6 | - |
N-acetylneuraminic acid | pH 7.0, 37°C | Lactiplantibacillus plantarum |