Cloned (Comment) | Organism |
---|---|
gene nanA, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 0.002-0.0025 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 0.0017-0.0022 ml of reservoir solution containing 25% w/v PEG 3350, 200 mM ammonium sulfate, 100 mM Bis-Tris pH 5.5, equilibration against 1 ml of reservoir solution, 8-20°C, method optimization, X-ray diffraction structure determination and analysis at 1.7 A resolution, molecular replacement | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + pyruvate | Staphylococcus aureus | the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate | N-acetylneuraminate | - |
r | |
N-acetyl-D-mannosamine + pyruvate | Staphylococcus aureus MRSA252 | the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate | N-acetylneuraminate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q6GK01 | subspecies aureus, methicillin-resistant, gene nanA | - |
Staphylococcus aureus MRSA252 | Q6GK01 | subspecies aureus, methicillin-resistant, gene nanA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, and gel filtration to over 95% purity | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
purified recombinant enzyme, pH 8.0, temperature not specified in the publication | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + pyruvate | the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate | Staphylococcus aureus | N-acetylneuraminate | - |
r | |
N-acetyl-D-mannosamine + pyruvate | the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate | Staphylococcus aureus MRSA252 | N-acetylneuraminate | - |
r | |
N-acetylneuraminate | - |
Staphylococcus aureus | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | - |
Staphylococcus aureus MRSA252 | N-acetyl-D-mannosamine + pyruvate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Staphylococcus aureus |