Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
D187E | increased Km | Clostridium perfringens |
D187N | decreased Vmax, increased Km | Clostridium perfringens |
E188D | cleaves 5-N-acetyl-9-O-acetylneuraminic acid as good as N-acetylneuraminic acid | Clostridium perfringens |
E188Q | cleaves 5-N-acetyl-9-O-acetylneuraminic acid as good as N-acetylneuraminic acid | Clostridium perfringens |
K161R | decreased Vmax | Clostridium perfringens |
Y133C | significant decrease in enzyme activity | Clostridium perfringens |
Y133F | significant decrease in enzyme activity | Clostridium perfringens |
Y133H | significant decrease in enzyme activity | Clostridium perfringens |
Y133W | significant decrease in enzyme activity | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-acetylneuraminic acid | pH 7.2, Y133F mutant | Clostridium perfringens | |
1 | - |
N-acetylneuraminic acid | pH 7.2, Y133H mutant | Clostridium perfringens | |
2 | - |
N-acetylneuraminic acid | pH 7.2, 50 mM phosphate buffer, wild-type | Clostridium perfringens | |
2.4 | - |
N-acetylneuraminic acid | pH 7.2, Y133C mutant | Clostridium perfringens | |
2.9 | - |
N-acetylneuraminic acid | pH 7.2, Y133W mutant | Clostridium perfringens | |
3.2 | - |
N-acetylneuraminic acid | pH 7.2, 250 mM phosphate buffer, wild-type | Clostridium perfringens | |
3.5 | - |
N-acetylneuraminic acid | pH 7.2, K161R mutant | Clostridium perfringens | |
17 | - |
N-acetylneuraminic acid | pH 7.2, E188Q mutant | Clostridium perfringens | |
29 | - |
N-acetylneuraminic acid | pH 7.2, D187E mutant | Clostridium perfringens | |
44 | - |
N-acetylneuraminic acid | pH 7.2, E188D mutant | Clostridium perfringens | |
1100 | - |
N-acetylneuraminic acid | pH 7.2, D187N mutant | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34600 | - |
SDS-PAGE | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Clostridium perfringens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
aceneuramate = N-acetyl-D-mannosamine + pyruvate | mechanism | Clostridium perfringens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.36 | - |
- |
Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-N-acetyl-9-O-acetylneuraminic acid | 54% the activity with N-acetylneuraminic acid | Clostridium perfringens | ? | - |
? | |
N-Acetylneuraminic acid | - |
Clostridium perfringens | N-Acetyl-D-mannosamine + pyruvate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65.7 | - |
- |
Clostridium perfringens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
increased activity after 10 min | Clostridium perfringens |
70 | - |
no loss of activity after 10 min | Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Clostridium perfringens |