Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 cells | Latilactobacillus sakei |
expressed in Escherichia coli Rosetta 2 cells | Limosilactobacillus antri |
Protein Variants | Comment | Organism |
---|---|---|
G210S/Y213G | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
G211S | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
P193Y | the mutant shows reduced activity compared to the wild type enzyme | Limosilactobacillus antri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.32 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
0.5 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.1 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.1 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.2 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
2 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
4.3 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
12.5 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
272 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
333 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
915 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
3200 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Latilactobacillus sakei |
140000 | - |
gel filtration | Limosilactobacillus antri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminate | Latilactobacillus sakei | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | Limosilactobacillus antri | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | Latilactobacillus sakei 23K | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | 100% activity | N-acetyl-D-mannosamine + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Latilactobacillus sakei | - |
- |
- |
Latilactobacillus sakei 23K | - |
- |
- |
Limosilactobacillus antri | C8P490 | - |
- |
Limosilactobacillus antri DSMZ 16041 | C8P490 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating affinity column chromatography | Latilactobacillus sakei |
Ni2+-chelating affinity column chromatography | Limosilactobacillus antri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + pyruvate | 1.2% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
2-deoxy-D-glucose + pyruvate | 33% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
2-deoxy-D-glucose + pyruvate | 33% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
2-deoxy-D-glucose + pyruvate | 1.2% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
D-arabinose + pyruvate | 1.0% activity compared to N-acetylneuraminate | Latilactobacillus sakei | 3-deoxy-D-manno-2-octulosonic acid | - |
r | |
D-arabinose + pyruvate | 1.5% activity compared to N-acetylneuraminate | Limosilactobacillus antri | 3-deoxy-D-manno-2-octulosonic acid | - |
r | |
D-mannose + pyruvate | 10% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
D-mannose + pyruvate | 95% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
D-mannose + pyruvate | 95% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
D-mannose + pyruvate | 10% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
D-xylose + pyruvate | 0.6% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
D-xylose + pyruvate | 2.4% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
D-xylose + pyruvate | 2.4% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
D-xylose + pyruvate | 0.6% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
L-gulose + pyruvate | 0.7% activity compared to N-acetylneuraminate | Limosilactobacillus antri | ? | - |
r | |
L-gulose + pyruvate | 3.6% activity compared to N-acetylneuraminate | Latilactobacillus sakei | ? | - |
r | |
L-gulose + pyruvate | 3.6% activity compared to N-acetylneuraminate | Latilactobacillus sakei 23K | ? | - |
r | |
L-gulose + pyruvate | 0.7% activity compared to N-acetylneuraminate | Limosilactobacillus antri DSMZ 16041 | ? | - |
r | |
N-acetylneuraminate | 100% activity | Latilactobacillus sakei | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | 100% activity | Limosilactobacillus antri | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | 100% activity | Latilactobacillus sakei 23K | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | 100% activity | Limosilactobacillus antri DSMZ 16041 | N-acetyl-D-mannosamine + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Latilactobacillus sakei |
homotetramer | 4 * 35000, SDS-PAGE | Limosilactobacillus antri |
homotetramer | 4 * 33500, electrospray ionization mass spectrometry | Latilactobacillus sakei |
homotetramer | 4 * 35700, electrospray ionization mass spectrometry | Limosilactobacillus antri |
Synonyms | Comment | Organism |
---|---|---|
NAL | - |
Latilactobacillus sakei |
NAL | - |
Limosilactobacillus antri |
Neu5Ac aldolase | - |
Latilactobacillus sakei |
Neu5Ac aldolase | - |
Limosilactobacillus antri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
in synthetic reaction | Latilactobacillus sakei |
50 | - |
in synthetic reaction | Latilactobacillus sakei |
60 | - |
in synthetic and hydrolytic reactions | Limosilactobacillus antri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
melting temperature in 100 mM phosphate buffer pH 7.0 | Latilactobacillus sakei |
65 | - |
melting temperature in 100 mM phosphate buffer pH 7.0 | Limosilactobacillus antri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.13 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.47 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.9 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
0.99 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.9 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
2.4 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
3 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
3.9 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
4.6 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
5.4 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
5.6 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
15.3 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Latilactobacillus sakei |
6 | 7 | - |
Limosilactobacillus antri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
N-acetyl-D-mannosamine | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.004 | - |
N-acetyl-D-mannosamine | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.016 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
0.016 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.026 | - |
N-acetylneuraminate | mutant enzyme G211S, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.23 | - |
N-acetylneuraminate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.31 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
0.48 | - |
N-acetylneuraminate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
0.71 | - |
pyruvate | mutant enzyme G210S/Y213G, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.7 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri | |
1.98 | - |
pyruvate | mutant enzyme P193Y, at pH 7.0 and 37°C | Limosilactobacillus antri | |
2.8 | - |
N-acetylneuraminate | wild type enzyme, at pH 7.0 and 37°C | Latilactobacillus sakei | |
5.1 | - |
pyruvate | wild type enzyme, at pH 7.0 and 37°C | Limosilactobacillus antri |