BRENDA - Enzyme Database show
show all sequences of 4.1.3.3

First functional and mutational analysis of group 3 N-acetylneuraminate lyases from Lactobacillus antri and Lactobacillus sakei 23K

Garcia-Garcia, M.I.; Gil-Ortiz, F.; Garcia-Carmona, F.; Sanchez-Ferrer, A.; PLoS ONE 9, e96976 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta 2 cells
Lactobacillus antri
expressed in Escherichia coli Rosetta 2 cells
Lactobacillus sakei
Engineering
Amino acid exchange
Commentary
Organism
G210S/Y213G
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
G211S
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
P193Y
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.32
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.5
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
1.1
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.1
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.2
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
2
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
4.3
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
12.5
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
272
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
333
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
915
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
3200
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
gel filtration
Lactobacillus antri
140000
-
gel filtration
Lactobacillus sakei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetylneuraminate
Lactobacillus sakei
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus antri
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus sakei 23K
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus antri DSMZ 16041
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus antri
C8P490
-
-
Lactobacillus antri DSMZ 16041
C8P490
-
-
Lactobacillus sakei
-
-
-
Lactobacillus sakei 23K
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-chelating affinity column chromatography
Lactobacillus antri
Ni2+-chelating affinity column chromatography
Lactobacillus sakei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + pyruvate
1.2% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
33% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
33% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
1.2% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
D-arabinose + pyruvate
1.0% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
3-deoxy-D-manno-2-octulosonic acid
-
-
-
r
D-arabinose + pyruvate
1.5% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
3-deoxy-D-manno-2-octulosonic acid
-
-
-
r
D-mannose + pyruvate
10% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
D-mannose + pyruvate
95% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
D-mannose + pyruvate
95% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
D-mannose + pyruvate
10% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
D-xylose + pyruvate
0.6% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
D-xylose + pyruvate
2.4% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
D-xylose + pyruvate
2.4% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
D-xylose + pyruvate
0.6% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
L-gulose + pyruvate
0.7% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
L-gulose + pyruvate
3.6% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
L-gulose + pyruvate
3.6% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
L-gulose + pyruvate
0.7% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus sakei
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus antri
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus sakei 23K
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus antri DSMZ 16041
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 35000, SDS-PAGE; 4 * 35700, electrospray ionization mass spectrometry
Lactobacillus antri
homotetramer
4 * 33500, electrospray ionization mass spectrometry; 4 * 35000, SDS-PAGE
Lactobacillus sakei
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
in synthetic reaction
Lactobacillus sakei
50
-
in synthetic reaction
Lactobacillus sakei
60
-
in synthetic and hydrolytic reactions
Lactobacillus antri
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
melting temperature in 100 mM phosphate buffer pH 7.0
Lactobacillus sakei
65
-
melting temperature in 100 mM phosphate buffer pH 7.0
Lactobacillus antri
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.032
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
0.13
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.47
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.9
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.99
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
1.9
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
2.4
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
3
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
3.9
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
4.6
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
5.4
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
5.6
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
15.3
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Lactobacillus antri
6
7
-
Lactobacillus sakei
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta 2 cells
Lactobacillus antri
expressed in Escherichia coli Rosetta 2 cells
Lactobacillus sakei
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G210S/Y213G
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
G211S
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
P193Y
the mutant shows reduced activity compared to the wild type enzyme
Lactobacillus antri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.32
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.5
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
1.1
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.1
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.2
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
2
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
4.3
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
12.5
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
272
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
333
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
915
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
3200
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
gel filtration
Lactobacillus antri
140000
-
gel filtration
Lactobacillus sakei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N-acetylneuraminate
Lactobacillus sakei
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus antri
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus sakei 23K
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
N-acetylneuraminate
Lactobacillus antri DSMZ 16041
100% activity
N-acetyl-D-mannosamine + pyruvate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-chelating affinity column chromatography
Lactobacillus antri
Ni2+-chelating affinity column chromatography
Lactobacillus sakei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + pyruvate
1.2% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
33% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
33% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
2-deoxy-D-glucose + pyruvate
1.2% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
D-arabinose + pyruvate
1.0% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
3-deoxy-D-manno-2-octulosonic acid
-
-
-
r
D-arabinose + pyruvate
1.5% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
3-deoxy-D-manno-2-octulosonic acid
-
-
-
r
D-mannose + pyruvate
10% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
D-mannose + pyruvate
95% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
D-mannose + pyruvate
95% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
D-mannose + pyruvate
10% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
D-xylose + pyruvate
0.6% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
D-xylose + pyruvate
2.4% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
D-xylose + pyruvate
2.4% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
D-xylose + pyruvate
0.6% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
L-gulose + pyruvate
0.7% activity compared to N-acetylneuraminate
749150
Lactobacillus antri
?
-
-
-
r
L-gulose + pyruvate
3.6% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei
?
-
-
-
r
L-gulose + pyruvate
3.6% activity compared to N-acetylneuraminate
749150
Lactobacillus sakei 23K
?
-
-
-
r
L-gulose + pyruvate
0.7% activity compared to N-acetylneuraminate
749150
Lactobacillus antri DSMZ 16041
?
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus sakei
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus antri
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus sakei 23K
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
100% activity
749150
Lactobacillus antri DSMZ 16041
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 35000, SDS-PAGE; 4 * 35700, electrospray ionization mass spectrometry
Lactobacillus antri
homotetramer
4 * 33500, electrospray ionization mass spectrometry; 4 * 35000, SDS-PAGE
Lactobacillus sakei
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
in synthetic reaction
Lactobacillus sakei
50
-
in synthetic reaction
Lactobacillus sakei
60
-
in synthetic and hydrolytic reactions
Lactobacillus antri
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
melting temperature in 100 mM phosphate buffer pH 7.0
Lactobacillus sakei
65
-
melting temperature in 100 mM phosphate buffer pH 7.0
Lactobacillus antri
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.032
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
0.13
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.47
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.9
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.99
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
1.9
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
2.4
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
3
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
3.9
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
4.6
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
5.4
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
5.6
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
15.3
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Lactobacillus antri
6
7
-
Lactobacillus sakei
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.002
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.004
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.016
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
0.016
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.026
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
0.23
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.31
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.48
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.71
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
1.7
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.98
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
2.8
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
5.1
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.002
-
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.004
-
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.016
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
0.016
-
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.026
-
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
Lactobacillus antri
0.23
-
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
0.31
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
0.48
-
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
0.71
-
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
Lactobacillus antri
1.7
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
1.98
-
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
Lactobacillus antri
2.8
-
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus sakei
5.1
-
pyruvate
wild type enzyme, at pH 7.0 and 37°C
Lactobacillus antri
Other publictions for EC 4.1.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748534
Wang
Molecular characterization of ...
Mycoplasma sp.
Mar. Drugs
16
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2018
2
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1
2
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2
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1
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4
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4
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1
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2
1
2
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1
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2
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In vivo multienzyme complex c ...
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2
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Structure and inhibition of N ...
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2016
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2
1
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1
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1
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1
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2
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2
1
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1
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749312
Ji
Characterization of a novel N ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Sci. Rep.
5
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2015
2
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1
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3
6
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5
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2
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3
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1
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-
-
-
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4
1
3
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1
6
2
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2
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1
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-
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3
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6
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5
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2
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1
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-
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4
1
3
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1
6
2
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726551
Daniels
Reaction mechanism of N-acetyl ...
Haemophilus influenzae
ACS Chem. Biol.
9
1025-1032
2014
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1
13
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2
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1
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3
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1
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2
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1
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2
1
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-
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-
1
13
-
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-
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2
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1
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-
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2
-
1
-
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2
1
-
-
-
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2
2
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2
2
749150
Garcia-Garcia
First functional and mutation ...
Lactobacillus antri, Lactobacillus antri DSMZ 16041, Lactobacillus sakei 23K, Lactobacillus sakei
PLoS ONE
9
e96976
2014
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2
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3
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13
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4
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2
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22
2
3
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2
13
2
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2
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3
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13
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2
4
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2
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-
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22
2
3
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2
13
2
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13
13
726631
North
Cloning, expression, purificat ...
Staphylococcus aureus, Staphylococcus aureus MRSA252
Acta Crystallogr. Sect. F
69
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2013
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4
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1
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1
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1
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2
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1
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1
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4
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1
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727035
Huynh
Structural basis for substrate ...
Pasteurella multocida
Biochemistry
52
8570-8579
2013
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1
1
1
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1
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3
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1
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-
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-
1
1
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1
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1
1
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1
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1
-
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-
1
1
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727186
Sun
Construction and expression of ...
Escherichia coli
Biores. Technol.
130
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2013
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1
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1
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6
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1
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1
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1
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1
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-
-
-
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1
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-
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1
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-
-
-
-
-
-
-
-
727317
Timms
Structural insights into the r ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
ChemBioChem
14
474-481
2013
-
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1
1
10
-
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5
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-
1
2
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4
-
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1
-
1
-
2
-
2
1
1
-
-
5
2
2
-
-
-
-
-
-
-
1
-
1
10
-
-
-
-
5
-
-
1
2
-
-
-
1
1
-
2
-
2
1
1
-
-
5
2
2
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-
-
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-
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-
727332
Zhou
Molecular cloning and characte ...
Staphylococcus hominis
Chin. J. Biotechnol.
29
480-489
2013
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1
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-
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3
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1
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1
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-
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1
1
1
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2
-
2
-
1
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-
-
-
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1
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
2
-
2
-
1
-
-
-
-
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3
3
728608
Xiao
Progesterone receptor-mediated ...
Mus musculus, Mus musculus B6/129S5-NplGt(IRESBetageo)332Lex/Mmucd
PLoS ONE
8
e65607
2013
-
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1
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4
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-
-
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3
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-
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1
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-
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-
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3
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-
-
-
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2
1
1
2
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727656
Garcia Garcia
Characterization of a novel N- ...
Staphylococcus carnosus, Staphylococcus carnosus TM300
J. Agric. Food Chem.
60
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2012
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1
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3
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5
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1
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1
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4
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2
1
4
3
1
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1
1
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-
-
-
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3
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-
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4
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1
-
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1
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4
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2
1
4
3
1
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3
3
713843
Sanchez-Carron
Molecular characterization of ...
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Appl. Environ. Microbiol.
77
2471-2478
2011
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1
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1
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3
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3
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1
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1
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1
1
2
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2
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1
1
1
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1
-
-
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1
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-
-
3
-
-
2
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-
-
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1
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1
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1
1
2
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2
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1
1
1
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3
3
713852
Gao
Chemoenzymatic synthesis of N- ...
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Appl. Environ. Microbiol.
77
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2011
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1
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1
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1
1
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1
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1
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714508
Garcia-Garcia
New stabilized FastPrep-CLEAs ...
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Biores. Technol.
102
6186-6191
2011
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1
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4
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2
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1
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2
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1
1
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4
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1
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2
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1
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2
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4
701855
Hu
Coupled bioconversion for prep ...
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Appl. Microbiol. Biotechnol.
85
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2010
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2
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1
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7
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5
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3
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1
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1
2
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-
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1
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1
-
-
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2
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5
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3
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1
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701881
Zhang
An efficient method for N-acet ...
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Appl. Microbiol. Biotechnol.
86
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2010
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1
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1
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1
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1
1
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1
1
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1
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1
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Campeotto
Structural insights into subst ...
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18
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1
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19
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1
1
17
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693709
Chu
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1
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701496
Campeotto
Structure of an Escherichia co ...
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1
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1
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11
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1
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18
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11
-
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1
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1
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2
1
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1
11
-
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1
1
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690576
Li
Pasteurella multocida sialic a ...
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79
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2
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6
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6
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Huang
Disaccharides as sialic acid a ...
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Paccalet
Engineering of a sialic acid s ...
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Production of 2-keto-3-deoxy-D ...
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6
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1
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Clostridium perfringens
Hoppe-Seyler's Z. Physiol. Chem.
357
839-853
1976
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33320
Barnett
The reaction of N-acetylneuram ...
Clostridium perfringens
Biochem. J.
143
487-490
1974
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33316
DeVries
N-acetylneuraminic acid aldola ...
Clostridium perfringens
Arch. Biochem. Biophys.
151
234-242
1972
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33326
Arden
Distribution of neuraminidase ...
Corynebacterium belfanti, Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, Corynebacterium ulcerans
J. Bacteriol.
112
1206-1212
1972
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33317
Barnett
Studies on N-acetylneuraminic ...
Clostridium perfringens
Biochem. J.
125
275-285
1971
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33328
Schauer
Studies on the substrate speci ...
Clostridium perfringens
Hoppe-Seyler's Z. Physiol. Chem.
352
1073-1080
1971
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33319
Sirbasku
Purification and properties of ...
Bos taurus
Biochim. Biophys. Acta
198
479-482
1970
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33330
Comb
The sialic acids. I. Structure ...
Escherichia coli, Rattus norvegicus
J. Biol. Chem.
235
2529-2537
1960
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