Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.3 extracted from

  • Garcia-Garcia, M.I.; Gil-Ortiz, F.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    First functional and mutational analysis of group 3 N-acetylneuraminate lyases from Lactobacillus antri and Lactobacillus sakei 23K (2014), PLoS ONE, 9, e96976 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 cells Latilactobacillus sakei
expressed in Escherichia coli Rosetta 2 cells Limosilactobacillus antri

Protein Variants

Protein Variants Comment Organism
G210S/Y213G the mutant shows reduced activity compared to the wild type enzyme Limosilactobacillus antri
G211S the mutant shows reduced activity compared to the wild type enzyme Limosilactobacillus antri
P193Y the mutant shows reduced activity compared to the wild type enzyme Limosilactobacillus antri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
0.32
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
0.5
-
pyruvate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
1.1
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
1.1
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
1.2
-
N-acetylneuraminate mutant enzyme G211S, at pH 7.0 and 37°C Limosilactobacillus antri
2
-
N-acetylneuraminate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
4.3
-
pyruvate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
12.5
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
272
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
333
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
915
-
N-acetyl-D-mannosamine mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
3200
-
N-acetyl-D-mannosamine mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
gel filtration Latilactobacillus sakei
140000
-
gel filtration Limosilactobacillus antri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetylneuraminate Latilactobacillus sakei 100% activity N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate Limosilactobacillus antri 100% activity N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate Latilactobacillus sakei 23K 100% activity N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate Limosilactobacillus antri DSMZ 16041 100% activity N-acetyl-D-mannosamine + pyruvate
-
r

Organism

Organism UniProt Comment Textmining
Latilactobacillus sakei
-
-
-
Latilactobacillus sakei 23K
-
-
-
Limosilactobacillus antri C8P490
-
-
Limosilactobacillus antri DSMZ 16041 C8P490
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating affinity column chromatography Latilactobacillus sakei
Ni2+-chelating affinity column chromatography Limosilactobacillus antri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + pyruvate 1.2% activity compared to N-acetylneuraminate Limosilactobacillus antri ?
-
r
2-deoxy-D-glucose + pyruvate 33% activity compared to N-acetylneuraminate Latilactobacillus sakei ?
-
r
2-deoxy-D-glucose + pyruvate 33% activity compared to N-acetylneuraminate Latilactobacillus sakei 23K ?
-
r
2-deoxy-D-glucose + pyruvate 1.2% activity compared to N-acetylneuraminate Limosilactobacillus antri DSMZ 16041 ?
-
r
D-arabinose + pyruvate 1.0% activity compared to N-acetylneuraminate Latilactobacillus sakei 3-deoxy-D-manno-2-octulosonic acid
-
r
D-arabinose + pyruvate 1.5% activity compared to N-acetylneuraminate Limosilactobacillus antri 3-deoxy-D-manno-2-octulosonic acid
-
r
D-mannose + pyruvate 10% activity compared to N-acetylneuraminate Limosilactobacillus antri ?
-
r
D-mannose + pyruvate 95% activity compared to N-acetylneuraminate Latilactobacillus sakei ?
-
r
D-mannose + pyruvate 95% activity compared to N-acetylneuraminate Latilactobacillus sakei 23K ?
-
r
D-mannose + pyruvate 10% activity compared to N-acetylneuraminate Limosilactobacillus antri DSMZ 16041 ?
-
r
D-xylose + pyruvate 0.6% activity compared to N-acetylneuraminate Limosilactobacillus antri ?
-
r
D-xylose + pyruvate 2.4% activity compared to N-acetylneuraminate Latilactobacillus sakei ?
-
r
D-xylose + pyruvate 2.4% activity compared to N-acetylneuraminate Latilactobacillus sakei 23K ?
-
r
D-xylose + pyruvate 0.6% activity compared to N-acetylneuraminate Limosilactobacillus antri DSMZ 16041 ?
-
r
L-gulose + pyruvate 0.7% activity compared to N-acetylneuraminate Limosilactobacillus antri ?
-
r
L-gulose + pyruvate 3.6% activity compared to N-acetylneuraminate Latilactobacillus sakei ?
-
r
L-gulose + pyruvate 3.6% activity compared to N-acetylneuraminate Latilactobacillus sakei 23K ?
-
r
L-gulose + pyruvate 0.7% activity compared to N-acetylneuraminate Limosilactobacillus antri DSMZ 16041 ?
-
r
N-acetylneuraminate 100% activity Latilactobacillus sakei N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate 100% activity Limosilactobacillus antri N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate 100% activity Latilactobacillus sakei 23K N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate 100% activity Limosilactobacillus antri DSMZ 16041 N-acetyl-D-mannosamine + pyruvate
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Latilactobacillus sakei
homotetramer 4 * 35000, SDS-PAGE Limosilactobacillus antri
homotetramer 4 * 33500, electrospray ionization mass spectrometry Latilactobacillus sakei
homotetramer 4 * 35700, electrospray ionization mass spectrometry Limosilactobacillus antri

Synonyms

Synonyms Comment Organism
NAL
-
Latilactobacillus sakei
NAL
-
Limosilactobacillus antri
Neu5Ac aldolase
-
Latilactobacillus sakei
Neu5Ac aldolase
-
Limosilactobacillus antri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
in synthetic reaction Latilactobacillus sakei
50
-
in synthetic reaction Latilactobacillus sakei
60
-
in synthetic and hydrolytic reactions Limosilactobacillus antri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
melting temperature in 100 mM phosphate buffer pH 7.0 Latilactobacillus sakei
65
-
melting temperature in 100 mM phosphate buffer pH 7.0 Limosilactobacillus antri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.032
-
N-acetylneuraminate mutant enzyme G211S, at pH 7.0 and 37°C Limosilactobacillus antri
0.13
-
N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
0.47
-
N-acetylneuraminate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
0.9
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
0.99
-
pyruvate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
1.9
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
2.4
-
N-acetyl-D-mannosamine mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
3
-
pyruvate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
3.9
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
4.6
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
5.4
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
5.6
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
15.3
-
N-acetyl-D-mannosamine mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Latilactobacillus sakei
6 7
-
Limosilactobacillus antri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
N-acetyl-D-mannosamine mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
0.004
-
N-acetyl-D-mannosamine mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
0.016
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
0.016
-
N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
0.026
-
N-acetylneuraminate mutant enzyme G211S, at pH 7.0 and 37°C Limosilactobacillus antri
0.23
-
N-acetylneuraminate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
0.31
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
0.48
-
N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
0.71
-
pyruvate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C Limosilactobacillus antri
1.7
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri
1.98
-
pyruvate mutant enzyme P193Y, at pH 7.0 and 37°C Limosilactobacillus antri
2.8
-
N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C Latilactobacillus sakei
5.1
-
pyruvate wild type enzyme, at pH 7.0 and 37°C Limosilactobacillus antri