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Literature summary for 4.1.3.3 extracted from

  • Sun, W.; Ji, W.; Li, N.; Tong, P.; Cheng, J.; He, Y.; Chen, Y.; Chen, X.; Wu, J.; Ouyang, P.; Xie, J.; Ying, H.
    Construction and expression of a polycistronic plasmid encoding N-acetylglucosamine 2-epimerase and N-acetylneuraminic acid lyase simultaneously for production of N-acetylneuraminic acid (2013), Biores. Technol., 130, 23-29.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene nal, co-expression in Escherichia coli strain Rosetta (DE3) pLysS with N-acetylglucosamine 2-epimerase from Synechocystis sp. PCC 6803 (snAGE) or Anabaena sp. CH1 (anAGE) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information synthesis of N-acetylneuraminic acid from N-acetylglucosamine and pyruvate by constructing and co-expressing a polycistronic plasmid encoding an N-acetylglucosamine 2-epimerase gene from Synechocystis sp. PCC 6803 (snAGE) or Anabaena sp. CH1 (anAGE) and the Escherichia coli N-acetylneuraminic acid lyase gene. Construction of four polycistronic plasmids in which the positions of AGE gene differ with respect to Nal gene, plasmid pET-28a-Nal-anAGE with anAGE gene located next to Nal gene produces the highest amount of N-acetylneuraminic acid, overview. anAGE from Anabaena sp. CH1 shows much higher activity and productivity than snAGE from Synechocystis sp. PCC 6803 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Synonyms

Synonyms Comment Organism
N-Acetylneuraminic acid lyase
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Escherichia coli
NAL
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli