Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be a useful and cheap biocatalyst to produce N-acetylneuraminic acid (Neu5Ac) | Staphylococcus carnosus |
Cloned (Comment) | Organism |
---|---|
gene ScNAL, sequence comparison, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) pLys | Staphylococcus carnosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 4 | pyruvate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
2 | - |
N-acetylneuraminate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
149 | - |
N-acetyl-D-mannosamine | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + pyruvate | Staphylococcus carnosus | reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine | N-acetylneuraminate | - |
r | |
N-acetyl-D-mannosamine + pyruvate | Staphylococcus carnosus TM300 | reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine | N-acetylneuraminate | - |
r | |
N-acetylneuraminate | Staphylococcus carnosus | - |
N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | Staphylococcus carnosus TM300 | - |
N-acetyl-D-mannosamine + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus carnosus | B9DIJ2 | - |
- |
Staphylococcus carnosus TM300 | B9DIJ2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 7.5fold from Escherichia coli strain Rosetta (DE3) pLys by ultrafiltration, nickel affinity chromatography, and gel filtration, to homogeneity | Staphylococcus carnosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.7 | - |
purified recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + pyruvate | reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine | Staphylococcus carnosus | N-acetylneuraminate | - |
r | |
N-acetyl-D-mannosamine + pyruvate | reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine | Staphylococcus carnosus TM300 | N-acetylneuraminate | - |
r | |
N-acetylneuraminate | - |
Staphylococcus carnosus | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | - |
Staphylococcus carnosus TM300 | N-acetyl-D-mannosamine + pyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAL | - |
Staphylococcus carnosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
synthesis reaction | Staphylococcus carnosus |
60 | - |
hydrolysis reaction | Staphylococcus carnosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | synthesis and hydrolysis reactions, profiles, overview | Staphylococcus carnosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified recombinant His-tagged enzyme, pH 7.0, completely stable over 140-160 min | Staphylococcus carnosus |
60 | - |
purified recombinant His-tagged enzyme, pH 7.0, 120 min, 60% activity remaining | Staphylococcus carnosus |
70 | - |
purified recombinant His-tagged enzyme, pH 7.0, inactivation within 60 min | Staphylococcus carnosus |
80 | - |
purified recombinant His-tagged enzyme, pH 7.0, inactivation within 10 min | Staphylococcus carnosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
pyruvate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
4 | - |
N-acetylneuraminate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
4.5 | - |
N-acetyl-D-mannosamine | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Staphylococcus carnosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-acetyl-D-mannosamine | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
0.21 | - |
pyruvate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus | |
2 | - |
N-acetylneuraminate | recombinant His-taggged enzyme, pH 7.0, 37°C | Staphylococcus carnosus |