Any feedback?
Literature summary for 4.1.3.3 extracted from
- Purification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli (1991), Biochem. J., 276, 541-546.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 7% residual activity at 1 mM | Escherichia coli |  |
| diethyldicarbonate | 28% residual activity at 1 mM | Escherichia coli | |
| dihydroxyacetone | 2% residual activity at 10 mM | Escherichia coli | |
| DL-glyceraldehyde | 0% residual activity at 10 mM | Escherichia coli | |
| Fe2+ | 78% residual activity at 1 mM | Escherichia coli | |
| N-bromosuccinimide | 0% residual activity at 0.1 mM, 36% residual activity at 0.01 mM | Escherichia coli | |
| NaBH4 | - |
Escherichia coli | |
| p-chloromercuribenzoate | 0% residual activity at 0.01 mM, 44% residual activity at 0.001 mM | Escherichia coli | |
| pyruvate | 30% residual activity at 10 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
N-acetylneuraminic acid | - |
Escherichia coli | |
| 3.3 | - |
N-glycolylneuraminic acid | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
3 * 35000, SDS-PAGE | Escherichia coli |
| 105000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to illumination in presence of Rose Bengal | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.5 | - |
N-acetylneuraminic acid | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-Glycolylneuraminic acid | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 35000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
pyruvate protects against heat inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | elimination | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | at 75°C stable for more than 15 min | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
