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Literature summary for 4.1.3.3 extracted from

  • Nees, S.; Schauer, R.; Mayer, F.
    Purification and characterization of N-acetylneuraminate lyase from Clostridium perfringens (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 839-853.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 70% residual activity at 1 mM Clostridium perfringens
Fe2+ 30% residual activity at 1 mM Clostridium perfringens
heavy metal ions
-
Clostridium perfringens
Hg2+ 0% residual activity at 1 mM Clostridium perfringens
pyruvate elimination Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information with substrate N-acetylneuraminic acid increasing Km with increasing pH Clostridium perfringens
2.8
-
N-acetylneuraminic acid
-
Clostridium perfringens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Clostridium perfringens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information
-
Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Clostridium perfringens
97000
-
gel filtration Clostridium perfringens
100000
-
ultracentrifugation Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium perfringens

Reaction

Reaction Comment Organism Reaction ID
aceneuramate = N-acetyl-D-mannosamine + pyruvate mechanism Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-mannosamine + pyruvate
-
Clostridium perfringens N-Acetylneuraminic acid
-
r
N-Acetylneuraminic acid
-
Clostridium perfringens N-Acetyl-D-mannosamine + pyruvate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
enzyme assay at Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.7
-
N-acetylneuraminic acid
-
Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
elimination Clostridium perfringens